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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
27/07/2007 |
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Autoria: |
RODRIGUES, L. R.; TERRA, T. F.; CATELLI, L. L.; ARIAS, C. A. A.; BERED, F.; BODANESE-ZANETTINI, M. H. |
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Título: |
Origin of embryo-like structures insoybean anther culture investigated using SSR marker. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. |
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Páginas: |
p. 251-252. |
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Série: |
(Embrapa Soja. Documentos, 228). |
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Idioma: |
Inglês |
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Notas: |
Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. |
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Conteúdo: |
The diploid origin of early calluses in soybean anther culture was recorded by some authors. Since the somatic tissues of mature soybean are known to be highly recalcitrant, it was assumed that, it was unlikely that embryos were derived from anther diploid tissues. However, in the course of our studies, embryo-like structures (ELS) originated from connective calluses were observed. That ELS were similar to those obtained from anthers, indicating the embryogenic ability of connective. The Satt418 microsatellite locus was examined in order to investigate the origin of ELS obtained from soybean anther culture. Four heterozygous plants derived from the cross BRQ96-3065 x BRSMG-Liderança were used as anther donors. A total of 7000 anthers were placed on the induction medium under culture conditions recommended to trigger androgenic response. After 60 days of culture, upper portion of 216 ELS were carefully cut and transferred to a proliferation medium, in order to obtain sufficient tissue for DNA extraction. Callogenic masses originated from 114 ELS were screened for the Satt418 microsatellite locus. ELS originated from plants number 1 and 2 were exclusively heterozygous. In plants 3 and 4, heterozygous and homozygous ELS were identified, suggesting occurrence of somatic embryogenesis and androgenesis in the same system. This unexpected morphogenic response seems to be genotype-dependent and confirm that anther culture is a limited system to trigger androgenesis in soybean, since ELS can be originated from diploid tissue (Supported by CNPq, FAPERGS and CAPES). MenosThe diploid origin of early calluses in soybean anther culture was recorded by some authors. Since the somatic tissues of mature soybean are known to be highly recalcitrant, it was assumed that, it was unlikely that embryos were derived from anther diploid tissues. However, in the course of our studies, embryo-like structures (ELS) originated from connective calluses were observed. That ELS were similar to those obtained from anthers, indicating the embryogenic ability of connective. The Satt418 microsatellite locus was examined in order to investigate the origin of ELS obtained from soybean anther culture. Four heterozygous plants derived from the cross BRQ96-3065 x BRSMG-Liderança were used as anther donors. A total of 7000 anthers were placed on the induction medium under culture conditions recommended to trigger androgenic response. After 60 days of culture, upper portion of 216 ELS were carefully cut and transferred to a proliferation medium, in order to obtain sufficient tissue for DNA extraction. Callogenic masses originated from 114 ELS were screened for the Satt418 microsatellite locus. ELS originated from plants number 1 and 2 were exclusively heterozygous. In plants 3 and 4, heterozygous and homozygous ELS were identified, suggesting occurrence of somatic embryogenesis and androgenesis in the same system. This unexpected morphogenic response seems to be genotype-dependent and confirm that anther culture is a limited system to trigger androgenesis in soybean, since... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02567naa a2200217 a 4500
001 1466825
005 2007-07-27
008 2004 bl uuuu u00u1 u #d
100 1 $aRODRIGUES, L. R.
245 $aOrigin of embryo-like structures insoybean anther culture investigated using SSR marker.
260 $c2004
300 $ap. 251-252.
490 $a(Embrapa Soja. Documentos, 228).
500 $aEditado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.
520 $aThe diploid origin of early calluses in soybean anther culture was recorded by some authors. Since the somatic tissues of mature soybean are known to be highly recalcitrant, it was assumed that, it was unlikely that embryos were derived from anther diploid tissues. However, in the course of our studies, embryo-like structures (ELS) originated from connective calluses were observed. That ELS were similar to those obtained from anthers, indicating the embryogenic ability of connective. The Satt418 microsatellite locus was examined in order to investigate the origin of ELS obtained from soybean anther culture. Four heterozygous plants derived from the cross BRQ96-3065 x BRSMG-Liderança were used as anther donors. A total of 7000 anthers were placed on the induction medium under culture conditions recommended to trigger androgenic response. After 60 days of culture, upper portion of 216 ELS were carefully cut and transferred to a proliferation medium, in order to obtain sufficient tissue for DNA extraction. Callogenic masses originated from 114 ELS were screened for the Satt418 microsatellite locus. ELS originated from plants number 1 and 2 were exclusively heterozygous. In plants 3 and 4, heterozygous and homozygous ELS were identified, suggesting occurrence of somatic embryogenesis and androgenesis in the same system. This unexpected morphogenic response seems to be genotype-dependent and confirm that anther culture is a limited system to trigger androgenesis in soybean, since ELS can be originated from diploid tissue (Supported by CNPq, FAPERGS and CAPES).
700 1 $aTERRA, T. F.
700 1 $aCATELLI, L. L.
700 1 $aARIAS, C. A. A.
700 1 $aBERED, F.
700 1 $aBODANESE-ZANETTINI, M. H.
773 $tIn: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.
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Embrapa Soja (CNPSO) |
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| 1. |  | SURITA, L. M. A.; FEIJO, G. L. D.; BONIN, M. N.; FERRAZ, A. L. J. Avaliação de atributos de qualidade de carne de novilhas cruzadas destinadas ao mercado de cortes nobres: dados preliminares. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 11., 2015, Campo Grande, MS. Anais... Campo Grande: Embrapa Gado de Corte, 2015. 114 p. (Embrapa Gado de Corte. Documentos, 213).| Tipo: Resumo em Anais de Congresso |
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