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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
01/08/2013 |
Data da última atualização: |
01/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CLEMENTE, Z.; CASTRO, V. L. S. S. de; FEITOSA, L. O.; LIMA, R.; JONSSON, C. M.; MAIA, A. de H. N.; FRACETO, L. F. |
Afiliação: |
ZAIRA CLEMENTE, IB-UNICAMP; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; L. O. FEITOSA, UFSCar; R. LIMA, UFSCar; CLAUDIO MARTIN JONSSON, CNPMA; ALINE DE HOLANDA NUNES MAIA, CNPMA; LEONARDO FERNANDES FRACETO, UNESP. |
Título: |
Fish exposure to nano-TiO2 under different experimental conditions: methodological aspects for nanoecotoxicology investigations. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Science of the Total Environment, Amsterdam, v. 463-464, p. 647-656, 2013. |
Idioma: |
Inglês |
Conteúdo: |
The ecotoxicology of nano-TiO2 has been extensively studied in recent years; however, few toxicological investigations have considered the photocatalytic properties of the substance, which can increase its toxicity to aquatic biota. The aim of this work was to evaluate the effects on fish exposed to different nano-TiO2 concentrations and illumination conditions. The interaction of these variables was investigated by observing the survival of the organisms, together with biomarkers of biochemical and genetic alterations. Fish (Piaractus mesopotamicus) were exposed for 96 h to 0, 1, 10, and 100 mg/L of nano-TiO2, under visible light, and visible light with ultraviolet (UV) light (22.47 J/cm2/h). The following biomarkers of oxidative stress were monitored in the liver: concentrations of lipid hydroperoxide and carbonylated protein, and specific activities of superoxide dismutase, catalase, and glutathione S-transferase. Other biomarkers of physiological function were also studied: the specific activities of acid phosphatase and Na,K-ATPase were analyzed in the liver and brain, respectively, and the concentration of metallothionein was measured in the gills. In addition, micronucleus and comet assays were performed with blood as genotoxic biomarkers. Nano-TiO2 caused no mortality under any of the conditions tested, but induced sublethal effects that were influenced by illumination condition. Under both illumination conditions tested, exposure to 100 mg/L showed an inhibition of acid phosphatase activity. Under visible light, there was an increase in metallothionein level in fish exposed to 1 mg/L of nano-TiO2. Under UV light, protein carbonylation was reduced in groups exposed to 1 and 10 mg/L, while nucleus alterations in erythrocytes were higher in fish exposed to 10 mg/L. As well as improving the understanding of nano-TiO2 toxicity, the findings demonstrated the importance of considering the experimental conditions in nanoecotoxicological tests. This work provides information for the development of protocols to study substances whose toxicity is affected by illumination conditions. MenosThe ecotoxicology of nano-TiO2 has been extensively studied in recent years; however, few toxicological investigations have considered the photocatalytic properties of the substance, which can increase its toxicity to aquatic biota. The aim of this work was to evaluate the effects on fish exposed to different nano-TiO2 concentrations and illumination conditions. The interaction of these variables was investigated by observing the survival of the organisms, together with biomarkers of biochemical and genetic alterations. Fish (Piaractus mesopotamicus) were exposed for 96 h to 0, 1, 10, and 100 mg/L of nano-TiO2, under visible light, and visible light with ultraviolet (UV) light (22.47 J/cm2/h). The following biomarkers of oxidative stress were monitored in the liver: concentrations of lipid hydroperoxide and carbonylated protein, and specific activities of superoxide dismutase, catalase, and glutathione S-transferase. Other biomarkers of physiological function were also studied: the specific activities of acid phosphatase and Na,K-ATPase were analyzed in the liver and brain, respectively, and the concentration of metallothionein was measured in the gills. In addition, micronucleus and comet assays were performed with blood as genotoxic biomarkers. Nano-TiO2 caused no mortality under any of the conditions tested, but induced sublethal effects that were influenced by illumination condition. Under both illumination conditions tested, exposure to 100 mg/L showed an inhibition of ... Mostrar Tudo |
Palavras-Chave: |
Nanotoxicity. |
Thesagro: |
Pacu; Peixe; Substância tóxica; Toxidez. |
Thesaurus Nal: |
Biomarkers; Ecotoxicology; Fish; Nanotechnology; Oxidative stress; Piaractus mesopotamicus; Titanium dioxide; Ultraviolet radiation. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03141naa a2200349 a 4500 001 1963123 005 2023-03-01 008 2013 bl uuuu u00u1 u #d 100 1 $aCLEMENTE, Z. 245 $aFish exposure to nano-TiO2 under different experimental conditions$bmethodological aspects for nanoecotoxicology investigations.$h[electronic resource] 260 $c2013 520 $aThe ecotoxicology of nano-TiO2 has been extensively studied in recent years; however, few toxicological investigations have considered the photocatalytic properties of the substance, which can increase its toxicity to aquatic biota. The aim of this work was to evaluate the effects on fish exposed to different nano-TiO2 concentrations and illumination conditions. The interaction of these variables was investigated by observing the survival of the organisms, together with biomarkers of biochemical and genetic alterations. Fish (Piaractus mesopotamicus) were exposed for 96 h to 0, 1, 10, and 100 mg/L of nano-TiO2, under visible light, and visible light with ultraviolet (UV) light (22.47 J/cm2/h). The following biomarkers of oxidative stress were monitored in the liver: concentrations of lipid hydroperoxide and carbonylated protein, and specific activities of superoxide dismutase, catalase, and glutathione S-transferase. Other biomarkers of physiological function were also studied: the specific activities of acid phosphatase and Na,K-ATPase were analyzed in the liver and brain, respectively, and the concentration of metallothionein was measured in the gills. In addition, micronucleus and comet assays were performed with blood as genotoxic biomarkers. Nano-TiO2 caused no mortality under any of the conditions tested, but induced sublethal effects that were influenced by illumination condition. Under both illumination conditions tested, exposure to 100 mg/L showed an inhibition of acid phosphatase activity. Under visible light, there was an increase in metallothionein level in fish exposed to 1 mg/L of nano-TiO2. Under UV light, protein carbonylation was reduced in groups exposed to 1 and 10 mg/L, while nucleus alterations in erythrocytes were higher in fish exposed to 10 mg/L. As well as improving the understanding of nano-TiO2 toxicity, the findings demonstrated the importance of considering the experimental conditions in nanoecotoxicological tests. This work provides information for the development of protocols to study substances whose toxicity is affected by illumination conditions. 650 $aBiomarkers 650 $aEcotoxicology 650 $aFish 650 $aNanotechnology 650 $aOxidative stress 650 $aPiaractus mesopotamicus 650 $aTitanium dioxide 650 $aUltraviolet radiation 650 $aPacu 650 $aPeixe 650 $aSubstância tóxica 650 $aToxidez 653 $aNanotoxicity 700 1 $aCASTRO, V. L. S. S. de 700 1 $aFEITOSA, L. O. 700 1 $aLIMA, R. 700 1 $aJONSSON, C. M. 700 1 $aMAIA, A. de H. N. 700 1 $aFRACETO, L. F. 773 $tScience of the Total Environment, Amsterdam$gv. 463-464, p. 647-656, 2013.
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Embrapa Meio Ambiente (CNPMA) |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
09/11/2006 |
Data da última atualização: |
14/05/2007 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
-- - A |
Autoria: |
TORAL, F. L. B; ALENCAR, M. M. de; FREITAS, A. R. de. |
Título: |
Arranjos para efeitos fixos e estruturas de (co)variâncias residuais para análises de medidas repetidas do peso de bovinos da raça Canchim. |
Ano de publicação: |
2006 |
Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 35, n.5, p.1951-1958, 2006. |
Idioma: |
Português |
Conteúdo: |
Este trabalho foi realizado com o objetivo de selecionar o arranjo para efeitos fixos e a estrutura de (co)variância residual que melhor representam a variabilidade dos pesos dentro do rebanho e dentro dos indivíduos, considerando-se dados de pesos de bovinos analisados como medidas repetidas. Foram utilizados dados de peso de 3.690 bovinos Canchim, obtidos ao nascimento, à desmama, aos 12 e aos 18 meses de idade. Analisaram-se diferentes arranjos para os efeitos fixos (grupos de contemporâneos e/ou efeitos principais de ano, mês ou época de nascimento e sexo do bezerro) e diferentes estruturas de (co)variâncias para os resíduos, considerando-se ou não alteração da variância residual ao longo da vida do animal e alteração da correlação entre as medidas tomadas em intervalos diferentes. Os resultados indicaram que o arranjo mais adequado dos efeitos fixos para representar a variabilidade dos pesos dos animais dentro do rebanho foi o grupo de contemporâneos formado por ano, mês e sexo do bezerro e que as melhores estruturas de (co)variâncias residuais foram a Fator Analítico de Primeira Ordem e a Não Estruturada, que consideram o aumento das variâncias com o aumento da idade do indivíduo e as correlações diferentes para cada par de medidas de peso. |
Palavras-Chave: |
Dados longitudinais; Efeitos principais; Grupos de contemporâneos; Resíduos correlacionados. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPPSE/16466/1/PROCIMMA2006.00106.pdf
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Marc: |
LEADER 01944naa a2200193 a 4500 001 1047594 005 2007-05-14 008 2006 bl uuuu u00u1 u #d 100 1 $aTORAL, F. L. B 245 $aArranjos para efeitos fixos e estruturas de (co)variâncias residuais para análises de medidas repetidas do peso de bovinos da raça Canchim. 260 $c2006 520 $aEste trabalho foi realizado com o objetivo de selecionar o arranjo para efeitos fixos e a estrutura de (co)variância residual que melhor representam a variabilidade dos pesos dentro do rebanho e dentro dos indivíduos, considerando-se dados de pesos de bovinos analisados como medidas repetidas. Foram utilizados dados de peso de 3.690 bovinos Canchim, obtidos ao nascimento, à desmama, aos 12 e aos 18 meses de idade. Analisaram-se diferentes arranjos para os efeitos fixos (grupos de contemporâneos e/ou efeitos principais de ano, mês ou época de nascimento e sexo do bezerro) e diferentes estruturas de (co)variâncias para os resíduos, considerando-se ou não alteração da variância residual ao longo da vida do animal e alteração da correlação entre as medidas tomadas em intervalos diferentes. Os resultados indicaram que o arranjo mais adequado dos efeitos fixos para representar a variabilidade dos pesos dos animais dentro do rebanho foi o grupo de contemporâneos formado por ano, mês e sexo do bezerro e que as melhores estruturas de (co)variâncias residuais foram a Fator Analítico de Primeira Ordem e a Não Estruturada, que consideram o aumento das variâncias com o aumento da idade do indivíduo e as correlações diferentes para cada par de medidas de peso. 653 $aDados longitudinais 653 $aEfeitos principais 653 $aGrupos de contemporâneos 653 $aResíduos correlacionados 700 1 $aALENCAR, M. M. de 700 1 $aFREITAS, A. R. de 773 $tRevista Brasileira de Zootecnia$gv. 35, n.5, p.1951-1958, 2006.
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