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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
05/04/2017 |
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Data da última atualização: |
10/07/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CLEMENTE, Z.; CASTRO, V. L. S. S. de; MARTINEZ, D. S. |
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Afiliação: |
Z. CLEMENTE; VERA LUCIA SCHERHOLZ S DE CASTRO, CNPMA; D. S. MARTINEZ. |
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Título: |
Influence of surface characteristics on graphene oxide toxicity for zebrafish embryo. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: SETAC EUROPE ANNUAL MEETING, 26., 2016, Nantes. Proceedings... Brussels: SETAC, 2016. Ref. TU044. |
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Páginas: |
p. 203. |
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Idioma: |
Inglês |
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Conteúdo: |
Despite nanotoxicological studies have been intensified in recent years, gaps remain in the methods used to assess the nanotechnology risks. These gaps are due to the complex nanomaterials behavior in the environment, especially in the presence of organic matter and depending on the nanomaterial characteristics. Studies indicate that humic acid present in the aquatic environment can increase the stability of nanomaterial dispersions and may change its toxicity to aquatic organisms. The adsorption of oxidative debris (carboxylated carbonaceous fragments - CCFs) has also been discussed as an important factor influencing GO properties and behavior. The surface characteristics of GO can influence its biotechnological application as well as its toxicological effects. The aim of this study was to evaluate the influence of the presence of oxidative debris and humic acid in the toxicity of graphene oxide (GO) utilizing Fish Embryo Toxicity Test (FET test). GO (Sigma Aldrich) was refluxed with NaOH (0.01M, 1h, 90oC) and HCl (0.1M, 1h, 90oC) to produce GO without debris (GOwd). GO And GOwd were characterized through spectrophotometry, dynamic light scattering and atomic force microscopy. Zebrafish embryos (Danio rerio) were exposed during 96 h to 100 mg.L-1 GO or GOwd, in presence or absence of humic acid (HA, 20 mg.L-1). A control group exposed to reconstituted water was also performed. At the end of the exposure period, the larvae were measured and frozen at -20oC for subsequent evaluation of acetylcholinesterase activity (AchE). We also performed an in vitro test to evaluate direct effect of the nanomaterial in zebrafish AchE activity. Homogenates of zebrafish larvae were incubated with GO and GOwd at 30 mg/L (20 min, 30oC) before AchE assay was performed. Both GO agglomerated and precipitated quickly in reconstituted water. The presence of HA in the medium stabilized the GO suspensions similarly to that occurred with GO in ultrapure water. There was no difference between groups related to the occurrence of embryo malformation or mortality. Larvae exposed to GO were shorter and showed lower AchE activity than control and group exposed to GOwd. The in vitro test showed that the nanomaterial did not inhibit AchE activity. The nanomaterial showed low toxicity to embryo, but the reduction in total length and AchE activity in the organisms can be due to indirect effects in zebrafish development. More experiments will be performed to understand those effects. MenosDespite nanotoxicological studies have been intensified in recent years, gaps remain in the methods used to assess the nanotechnology risks. These gaps are due to the complex nanomaterials behavior in the environment, especially in the presence of organic matter and depending on the nanomaterial characteristics. Studies indicate that humic acid present in the aquatic environment can increase the stability of nanomaterial dispersions and may change its toxicity to aquatic organisms. The adsorption of oxidative debris (carboxylated carbonaceous fragments - CCFs) has also been discussed as an important factor influencing GO properties and behavior. The surface characteristics of GO can influence its biotechnological application as well as its toxicological effects. The aim of this study was to evaluate the influence of the presence of oxidative debris and humic acid in the toxicity of graphene oxide (GO) utilizing Fish Embryo Toxicity Test (FET test). GO (Sigma Aldrich) was refluxed with NaOH (0.01M, 1h, 90oC) and HCl (0.1M, 1h, 90oC) to produce GO without debris (GOwd). GO And GOwd were characterized through spectrophotometry, dynamic light scattering and atomic force microscopy. Zebrafish embryos (Danio rerio) were exposed during 96 h to 100 mg.L-1 GO or GOwd, in presence or absence of humic acid (HA, 20 mg.L-1). A control group exposed to reconstituted water was also performed. At the end of the exposure period, the larvae were measured and frozen at -20oC for subsequent eva... Mostrar Tudo |
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Palavras-Chave: |
Graphne; Zebrafish. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161626/1/clemente-influence.pdf
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Marc: |
LEADER 03052nam a2200169 a 4500
001 2068135
005 2017-07-10
008 2016 bl uuuu u00u1 u #d
100 1 $aCLEMENTE, Z.
245 $aInfluence of surface characteristics on graphene oxide toxicity for zebrafish embryo.$h[electronic resource]
260 $aIn: SETAC EUROPE ANNUAL MEETING, 26., 2016, Nantes. Proceedings... Brussels: SETAC, 2016. Ref. TU044.$c2016
300 $ap. 203.
520 $aDespite nanotoxicological studies have been intensified in recent years, gaps remain in the methods used to assess the nanotechnology risks. These gaps are due to the complex nanomaterials behavior in the environment, especially in the presence of organic matter and depending on the nanomaterial characteristics. Studies indicate that humic acid present in the aquatic environment can increase the stability of nanomaterial dispersions and may change its toxicity to aquatic organisms. The adsorption of oxidative debris (carboxylated carbonaceous fragments - CCFs) has also been discussed as an important factor influencing GO properties and behavior. The surface characteristics of GO can influence its biotechnological application as well as its toxicological effects. The aim of this study was to evaluate the influence of the presence of oxidative debris and humic acid in the toxicity of graphene oxide (GO) utilizing Fish Embryo Toxicity Test (FET test). GO (Sigma Aldrich) was refluxed with NaOH (0.01M, 1h, 90oC) and HCl (0.1M, 1h, 90oC) to produce GO without debris (GOwd). GO And GOwd were characterized through spectrophotometry, dynamic light scattering and atomic force microscopy. Zebrafish embryos (Danio rerio) were exposed during 96 h to 100 mg.L-1 GO or GOwd, in presence or absence of humic acid (HA, 20 mg.L-1). A control group exposed to reconstituted water was also performed. At the end of the exposure period, the larvae were measured and frozen at -20oC for subsequent evaluation of acetylcholinesterase activity (AchE). We also performed an in vitro test to evaluate direct effect of the nanomaterial in zebrafish AchE activity. Homogenates of zebrafish larvae were incubated with GO and GOwd at 30 mg/L (20 min, 30oC) before AchE assay was performed. Both GO agglomerated and precipitated quickly in reconstituted water. The presence of HA in the medium stabilized the GO suspensions similarly to that occurred with GO in ultrapure water. There was no difference between groups related to the occurrence of embryo malformation or mortality. Larvae exposed to GO were shorter and showed lower AchE activity than control and group exposed to GOwd. The in vitro test showed that the nanomaterial did not inhibit AchE activity. The nanomaterial showed low toxicity to embryo, but the reduction in total length and AchE activity in the organisms can be due to indirect effects in zebrafish development. More experiments will be performed to understand those effects.
653 $aGraphne
653 $aZebrafish
700 1 $aCASTRO, V. L. S. S. de
700 1 $aMARTINEZ, D. S.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 212 | |
| 106. |  | CLEMENTE, Z.; MARTINEZ, D. S. T.; CASTRO, V. L. S. S. de. Perspectivas do uso do teste com embriões de zebrafish no âmbito da nanotoxicologia. Revista da Sociedade Brasileira de Ciências em Animais de Laboratório, São Paulo, v. 4, n. 1, p. 67, 2016. Edição dos anais do 14º Congresso da Sociedade Brasileira de Ciências em Animais de Laboratório e do 3º Encontro Latino Americano de Ciências em Animais de Laboratório, 2016, Porto Alegre. Pôster.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 108. | | DANTZGER, M.; DANTZGER, D. D.; JONSSON, C. M.; CASTRO, V. L. S. S. de; AOYAMA, H. The potential use of crustacean phosphatases as a biosensor to monitor metals in environmental samples. INTERNATIONAL CONFERENCE ON FOOD AND AGRICULTURE APPLICATIONS OF NANOTECHNOLOGIES - NanoAgri, 2010, São Pedro, SP. [Anais...] São Pedro: Aptor Software, 2010. Editors: Caue Ribeiro, Odílio Benedito Garrido de Assis, Luiz Henrique Capparelli Mattoso, Sérgio Mascarenhas.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 114. | | PRESTES, E. B.; PAULA, V. J. de; SANTOS, F.; JONSSON, C. M.; CASTRO, V. L. S. S. de. Avaliação do efeito dos fungicidas Epoxiconazol, Piraclostrobina e sua mistura no crescimento da microalga Pseudokirchneriella subcapitata. In: CONGRESSO BRASILEIRO DE ECOTOXICOLOGIA, 11., 2010, Bombinhas. Anais... Bombinhas: Sociedade Brasileira de Ecotoxicologia, 2010. 1 CD- ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 115. | | OLIVEIRA, L. P. de; LIMA, C. G. de; SIQUEIRA, M. C.; CASTRO, V. L. S. S. de. Ajuste de modelo heteroscedástico em estudo toxicológico usando o SAS. Revista da Estatística UFOP, Ouro Preto, v. 3, n. 3, 2014. Edição dos trabalhos apresentados como pôsteres na 59ª Reunião Anual da Regional Brasileira da Sociedade Internacional de Biometria, Ouro Preto, 2014. p. 49-53.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 118. | | MEDEIROS, A. M. Z.; SILVA, G. H. da; CASTRO, V. L. S. S. de; MARTINEZ, D. S. T. Efeitos diferentes na co-exposição de óxido de grafeno e ácido húmico em zebrafish: influência do córion. In: SIMPÓSIO CIENTÍFICO DOS PÓS-GRADUANDOS DO CENA, 10., 2017, Piracicaba. Novos tempos na pesquisa: transformação, liderança e inovação: resumos... Piracicaba, SP: CENA/USP, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 212 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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