Data/hora: 20/06/2024 22:20:50

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 01/02/2016
Data da última atualização: | 01/02/2024
Autoria: | CASTRO, R. de J. C.; SOUZA SOBRINHO, F. de; GAMA, M. A. S. da; SATO, R. T.; LIMA, L. L.; OLIVEIRA, M. A. L. de.
Afiliação: | Renata de Jesus Coelho Castro, UFJF; FAUSTO DE SOUZA SOBRINHO, CNPGL; MARCO ANTONIO SUNDFELD DA GAMA, CNPGL; Renata Takabayashi Sato, UFJF; Larissa Lavorato Lima, UFJF; Marcone Augusto Leal de Oliveira, UFJF.
Título: | A Rapid Method for Determination of the Main Conjugated Linoleic Acid Precursors (C18:2 n-6 and C18:3 n-3) in Forage by Capillary Zone Electrophoresis with Ultraviolet Detection Using Gas Chromatography with Flame Ionization Detection as a Comparative Method.
Ano de publicação: | 2015
Fonte/Imprenta: | Journal of AOAC International, v. 98, n. 6, p. 1591-1597, 2015.
Idioma: | Inglês
Conteúdo: | Abstract: A rapid method has been proposed for determination of the main conjugated linoleic acid precursors such as linoleic (C18:2 n-6) and linolenic (C18:3 n-3) acids in forages by capillary zone electrophoresis (CZE) with direct UV detection at 200 nm. Among the fatty acids found in forages, C18:2 n-6 and C18:3 n-3 have received particular attention due to their roles as precursors for the synthesis of conjugated linoleic acid, a class of health-enhancing compounds that is predominantly found in dairy products. The electrolyte background consisted of 12.0 mmol/L tetraborate buffer (pH 9.2) added to 12.0 mmol/L Brij 35(®), 17% acetonitrile, and 33% methanol. Under the optimized conditions, the baseline separation of C18:2 n-6 and C18:3 n-3 was achieved within 4 min. The CZE-UV method was compared to GC with a flame ionization detector, which is the American Oil Chemists' Society (AOCS 996.06) official method for fatty acid analysis. The methods did not show any evidence of significant differences within 95% confidence interval (P > 0.05). The CZE-UV method was successfully applied to the analysis of 80 genotypes of Brachiaria ruzizienses clones submitted to a genetic improvement program in agricultural research.
Palavras-Chave: | CLA; CZE-UV method; Fatty acids in forages.
Thesaurus Nal: | conjugated linoleic acid.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (22305-1) - UPC - AP - DD -  -  -  - DIS