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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/11/1999 |
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Data da última atualização: |
04/07/2016 |
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Autoria: |
CASTRO, R. S.; LEITE, R. C.; RESENDE, M.; MARTINS, A.; GOUVEIA, A. M. G. |
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Título: |
Isolamento e identificação pela imunofluorescência direta e reação em cadeia de polimerase do vírus da artrite-encefalite caprina. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, v. 51, n. 3, p. 235-240, 1999. |
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DOI: |
http://dx.doi.org/10.1590/S0102-09351999000300006 |
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Idioma: |
Português |
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Conteúdo: |
Amostras do vírus da artrite-encefalite caprina (CAEV) de animais dos estados de Minas Gerais e Pernambuco foram isoladas a partir de explantes de membrana sinovial (MS) (amostras BrMg 1-01, BrMg 2-01, BrMg 2-02, BrMg 2-03 e BrPe 1-01) ou de co-cultivo de leucócitos com MS (BrMg 1-02). As amostras foram identificadas pelo efeito citopático-ECP (formação de sincícios), imunofluorescência direta (IFD) e reação em cadeia de polimerase (PCR) aperfeiçoada para amplificação de parte do gene gag da amostra CAEV Cork. O estudo do ECP revelou a presença de amostras pouco (BrMg 1-01 e BrMg 1-02) ou fortemente indutoras de ECP (BrMg 2-01, BrMg 2-02, BrMg 2-03 e BrPe 1-01). A IFD mostrou resultado positivo em células de todas as monocamadas infectadas pelas amostras isoladas e em células de referência (CAEV Cork e visna/maedi K 1514). A PCR do DNA de células infectadas resultou na amplificação específica de um fragmento de DNA de 286pb da amostras testadas, exceto do visna/maedi K 1514.
Caprine arthritis encephalitis virus isolation and identification using fluorescent antibody and polimerase chain reaction.
This paper describes the isolation of caprine arthritis-encephalitis virus (CAEV) in Minas Gerais and Pernambuco States, Brazil, using goat synovial membrane (GSM) explants (isolates: BrMg 1-01, BrMg 2-01, BrMg 2-02, BrMg 2-03 and BrPe 1-01) or co-culture of blood mononuclear cells with GSM (BrMg 1-02). The isolates were identified by cytopathic effect (CPE), direct immunofluorescence (DIF) and an improved polimerase chain reaction (PRC) specific to CAEV (strain Cork) genome. It was found isolates of low (BrMg 1-01 and BrMg 1-02) and of high lytic (BrMg 2-01, BrMg 2-02, BrMg 2-03 and BrPe 1-01) phenotypes. The DIF revealed positive results in cells infected by all isolates tested and the reference strains of CAEV (Cork) and visna-maedi (K1514). The used PCR amplified a 286 pb fragment of DNA from cells infected with all isolates and CAEV Cork but not with visna-maedi K1514. MenosAmostras do vírus da artrite-encefalite caprina (CAEV) de animais dos estados de Minas Gerais e Pernambuco foram isoladas a partir de explantes de membrana sinovial (MS) (amostras BrMg 1-01, BrMg 2-01, BrMg 2-02, BrMg 2-03 e BrPe 1-01) ou de co-cultivo de leucócitos com MS (BrMg 1-02). As amostras foram identificadas pelo efeito citopático-ECP (formação de sincícios), imunofluorescência direta (IFD) e reação em cadeia de polimerase (PCR) aperfeiçoada para amplificação de parte do gene gag da amostra CAEV Cork. O estudo do ECP revelou a presença de amostras pouco (BrMg 1-01 e BrMg 1-02) ou fortemente indutoras de ECP (BrMg 2-01, BrMg 2-02, BrMg 2-03 e BrPe 1-01). A IFD mostrou resultado positivo em células de todas as monocamadas infectadas pelas amostras isoladas e em células de referência (CAEV Cork e visna/maedi K 1514). A PCR do DNA de células infectadas resultou na amplificação específica de um fragmento de DNA de 286pb da amostras testadas, exceto do visna/maedi K 1514.
Caprine arthritis encephalitis virus isolation and identification using fluorescent antibody and polimerase chain reaction.
This paper describes the isolation of caprine arthritis-encephalitis virus (CAEV) in Minas Gerais and Pernambuco States, Brazil, using goat synovial membrane (GSM) explants (isolates: BrMg 1-01, BrMg 2-01, BrMg 2-02, BrMg 2-03 and BrPe 1-01) or co-culture of blood mononuclear cells with GSM (BrMg 1-02). The isolates were identified by cytopathic effect (CPE), direct immunofluoresc... Mostrar Tudo |
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Palavras-Chave: |
Artrite encefalite. |
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Thesagro: |
Caprino. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02717naa a2200205 a 4500
001 1519065
005 2016-07-04
008 1999 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/S0102-09351999000300006$2DOI
100 1 $aCASTRO, R. S.
245 $aIsolamento e identificação pela imunofluorescência direta e reação em cadeia de polimerase do vírus da artrite-encefalite caprina.
260 $c1999
520 $aAmostras do vírus da artrite-encefalite caprina (CAEV) de animais dos estados de Minas Gerais e Pernambuco foram isoladas a partir de explantes de membrana sinovial (MS) (amostras BrMg 1-01, BrMg 2-01, BrMg 2-02, BrMg 2-03 e BrPe 1-01) ou de co-cultivo de leucócitos com MS (BrMg 1-02). As amostras foram identificadas pelo efeito citopático-ECP (formação de sincícios), imunofluorescência direta (IFD) e reação em cadeia de polimerase (PCR) aperfeiçoada para amplificação de parte do gene gag da amostra CAEV Cork. O estudo do ECP revelou a presença de amostras pouco (BrMg 1-01 e BrMg 1-02) ou fortemente indutoras de ECP (BrMg 2-01, BrMg 2-02, BrMg 2-03 e BrPe 1-01). A IFD mostrou resultado positivo em células de todas as monocamadas infectadas pelas amostras isoladas e em células de referência (CAEV Cork e visna/maedi K 1514). A PCR do DNA de células infectadas resultou na amplificação específica de um fragmento de DNA de 286pb da amostras testadas, exceto do visna/maedi K 1514. Caprine arthritis encephalitis virus isolation and identification using fluorescent antibody and polimerase chain reaction. This paper describes the isolation of caprine arthritis-encephalitis virus (CAEV) in Minas Gerais and Pernambuco States, Brazil, using goat synovial membrane (GSM) explants (isolates: BrMg 1-01, BrMg 2-01, BrMg 2-02, BrMg 2-03 and BrPe 1-01) or co-culture of blood mononuclear cells with GSM (BrMg 1-02). The isolates were identified by cytopathic effect (CPE), direct immunofluorescence (DIF) and an improved polimerase chain reaction (PRC) specific to CAEV (strain Cork) genome. It was found isolates of low (BrMg 1-01 and BrMg 1-02) and of high lytic (BrMg 2-01, BrMg 2-02, BrMg 2-03 and BrPe 1-01) phenotypes. The DIF revealed positive results in cells infected by all isolates tested and the reference strains of CAEV (Cork) and visna-maedi (K1514). The used PCR amplified a 286 pb fragment of DNA from cells infected with all isolates and CAEV Cork but not with visna-maedi K1514.
650 $aCaprino
653 $aArtrite encefalite
700 1 $aLEITE, R. C.
700 1 $aRESENDE, M.
700 1 $aMARTINS, A.
700 1 $aGOUVEIA, A. M. G.
773 $tArquivo Brasileiro de Medicina Veterinaria e Zootecnia$gv. 51, n. 3, p. 235-240, 1999.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Arroz e Feijão; Embrapa Clima Temperado. |
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Data corrente: |
13/03/2020 |
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Data da última atualização: |
07/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
STEINMETZ, S.; CUADRA, S. V.; ALMEIDA, I. R. de; STRECK, N. A.; ZANON, A. J.; RIBAS, G. G.; SILVA, M. R. da; BENEDETTI, R. P.; CERA, J. C.; SILVA, S. C. da; HEINEMANN, A. B. |
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Afiliação: |
SILVIO STEINMETZ, CPACT; SANTIAGO VIANNA CUADRA, CPACT; IVAN RODRIGUES DE ALMEIDA, CPACT; NEREU AUGUSTO STRECK; ALENCAR JÚNIOR ZANON; GIOVANA GHISLENE RIBAS; MICHEL ROCHA DA SILVA; RÔMULO PULCINELLI BENEDETTI; JOSSANA CEOLIN CERA; SILVANDO CARLOS DA SILVA, CNPAF; ALEXANDRE BRYAN HEINEMANN, CNPAF. |
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Título: |
Irrigated rice sowing periods based on simulated grain yield. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Agrometeoros, Passo Fundo, v. 27, n. 2, p. 377-386, dez. 2019. |
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ISSN: |
2526-7043 |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT. This study aimed to establish the sowing periods with lower climatic risk for irrigated rice cultivation in the State of Rio Grande do Sul, Brazil, using the grain yield simulated by the SimulArroz which is an eco-physiological model based in processes that also simulates the development and the growth of the crop. The simulated grain yields were normalized for each of the four groups of cultivars, referenced as Relative Yield (YRel), considering the values of Reference Yield (YRef). The YRef considered was equal to the 80 percentile for the cultivars of groups 1 and 2 (Very short and Short cycle, respectively), and to the 90 percentile, for the cultivars of groups 3 and 4 (Medium and Long cycle, respectively). A successful year was when the YRel value was equal to or greater than 60% of YRef. These years were used to classify the climatic risk levels of 20% (80% success), 30% (70% success) and 40% (60% success). The preferential sowing periods for the four cultivar groups, at three levels of climatic risk, were established for all counties of the State of Rio Grande do Sul. The results indicated that the beginning of sowing occurs, depending on the group of cultivars, on September 1st or September 11th. On the other hand, the final sowing date varies with the group of cultivars and the level of risk involved, being December 20th the last sowing date for group 1 (Very short cycle). It is concluded that the SimulArroz model is appropriate to define the sowing periods of irrigated rice in Rio Grande do Sul considering grain yield, cycle of the cultivars and level of climatic risk. MenosABSTRACT. This study aimed to establish the sowing periods with lower climatic risk for irrigated rice cultivation in the State of Rio Grande do Sul, Brazil, using the grain yield simulated by the SimulArroz which is an eco-physiological model based in processes that also simulates the development and the growth of the crop. The simulated grain yields were normalized for each of the four groups of cultivars, referenced as Relative Yield (YRel), considering the values of Reference Yield (YRef). The YRef considered was equal to the 80 percentile for the cultivars of groups 1 and 2 (Very short and Short cycle, respectively), and to the 90 percentile, for the cultivars of groups 3 and 4 (Medium and Long cycle, respectively). A successful year was when the YRel value was equal to or greater than 60% of YRef. These years were used to classify the climatic risk levels of 20% (80% success), 30% (70% success) and 40% (60% success). The preferential sowing periods for the four cultivar groups, at three levels of climatic risk, were established for all counties of the State of Rio Grande do Sul. The results indicated that the beginning of sowing occurs, depending on the group of cultivars, on September 1st or September 11th. On the other hand, the final sowing date varies with the group of cultivars and the level of risk involved, being December 20th the last sowing date for group 1 (Very short cycle). It is concluded that the SimulArroz model is appropriate to define the sowing period... Mostrar Tudo |
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Thesagro: |
Arroz; Arroz Irrigado; Clima; Oryza Sativa; Risco Climático. |
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Thesaurus NAL: |
Agricultural zoning; Climate; Crop models; Rice. |
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Categoria do assunto: |
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X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211846/1/STEINMETZ2019-26440-127189-1-PB.pdf
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Marc: |
LEADER 02567naa a2200361 a 4500
001 2121230
005 2020-04-07
008 2019 bl uuuu u00u1 u #d
022 $a2526-7043
100 1 $aSTEINMETZ, S.
245 $aIrrigated rice sowing periods based on simulated grain yield.$h[electronic resource]
260 $c2019
520 $aABSTRACT. This study aimed to establish the sowing periods with lower climatic risk for irrigated rice cultivation in the State of Rio Grande do Sul, Brazil, using the grain yield simulated by the SimulArroz which is an eco-physiological model based in processes that also simulates the development and the growth of the crop. The simulated grain yields were normalized for each of the four groups of cultivars, referenced as Relative Yield (YRel), considering the values of Reference Yield (YRef). The YRef considered was equal to the 80 percentile for the cultivars of groups 1 and 2 (Very short and Short cycle, respectively), and to the 90 percentile, for the cultivars of groups 3 and 4 (Medium and Long cycle, respectively). A successful year was when the YRel value was equal to or greater than 60% of YRef. These years were used to classify the climatic risk levels of 20% (80% success), 30% (70% success) and 40% (60% success). The preferential sowing periods for the four cultivar groups, at three levels of climatic risk, were established for all counties of the State of Rio Grande do Sul. The results indicated that the beginning of sowing occurs, depending on the group of cultivars, on September 1st or September 11th. On the other hand, the final sowing date varies with the group of cultivars and the level of risk involved, being December 20th the last sowing date for group 1 (Very short cycle). It is concluded that the SimulArroz model is appropriate to define the sowing periods of irrigated rice in Rio Grande do Sul considering grain yield, cycle of the cultivars and level of climatic risk.
650 $aAgricultural zoning
650 $aClimate
650 $aCrop models
650 $aRice
650 $aArroz
650 $aArroz Irrigado
650 $aClima
650 $aOryza Sativa
650 $aRisco Climático
700 1 $aCUADRA, S. V.
700 1 $aALMEIDA, I. R. de
700 1 $aSTRECK, N. A.
700 1 $aZANON, A. J.
700 1 $aRIBAS, G. G.
700 1 $aSILVA, M. R. da
700 1 $aBENEDETTI, R. P.
700 1 $aCERA, J. C.
700 1 $aSILVA, S. C. da
700 1 $aHEINEMANN, A. B.
773 $tAgrometeoros, Passo Fundo$gv. 27, n. 2, p. 377-386, dez. 2019.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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