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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Corte. |
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Data corrente: |
04/02/2009 |
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Data da última atualização: |
22/07/2011 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, S. R. de O.; MOTA, R. A.; ROSINHA, G. M. S.; FORNER, O.; PINHEIRO JÚNIOR, J. W.; PEREIRA, R. R. B.; CASTRO, R. S. de; ELISEI, C.; SOARES, C. O.; ARAÚJO, F. R.; MADUREIRA, R. C. |
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Afiliação: |
Sílvio Romero de O. Abreu, CESMAC; Rinaldo A. Mota, UFRPB; Grácia Maria Soares Rosinha, CNPGC; Odinéia Forner, bolsista CNPGC; José W. Pinheiro Júnior, UFRPB; Renata Ribeiro Bastos Pereira, bolsista CNPGC; Roberto S. de Castro, UFRPR; Carina Elisei, Pesquisadora DCR do CNPq/FUNDECT no CNPGC; Cleber Oliveira Soares, CNPGC; Flábio Ribeiro Araújo, CNPGC; Renata Cunha Madureira, CNPGC. |
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Título: |
Comparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Seropédica, v. 28, n. 10, p. 481-487, out. 2008. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região.
[Genotypic comparison between Corynebacterium pseudotuberculosis samples obtained from sheep and goats with caseous lymphadenitis, raised in the semi-arid region of Pernambuco].
Abstract: The objective was to genotypically compare 35 samples of Corynebacterium pseudotuberculosis obtained from abscesses of sheep and goats diagnosed with caseous lymphadenitis originated from 5 different municipalities in the semi-arid region of Pernambuco, Brazil. The RFLP-PCR technique with Hpy-Ch4 and Msp I and Pst I Msp I restriction enzimes was used to fingerprint the genes rpoB and pld, respectively. The results demonstrate that there was no difference on the fragments banding pattern among samples, independently of the host species or geographic area studied, defining a homogeneous profile of C. pseudotuberculosis responsible for superficial abscesses for the region. MenosResumo: Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região.
[Genotypic comparison between Corynebacterium pseudotuberculosis samples obtained from sheep and goats with caseous lymphadenitis, raised in the semi-arid region of Pernambuco].
Abstract: The objective was to genotypically compare 35 samples of Corynebacterium pseudotuberculosis obtained from abscesses of sheep and goats diagnosed with caseous lymphadenitis originated from 5 different municipalities in the semi-arid region of Pernambuco, Brazil. The RFLP-PCR technique with Hpy-Ch4 and Msp I and Pst I Msp I restriction enzimes was used to fingerprint the genes rpoB and pld, respectively. The results demonstrate that there was no difference on the fragments banding pattern among samples, independently of the host species or geographic area studied, defining a homogeneous profile of C. pseudotuberculos... Mostrar Tudo |
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Palavras-Chave: |
Pernambuco; RFLP-PCR. |
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Thesagro: |
Caprino; Corynebacterium pseudotuberculosis; Epidemiologia; Linfadenite caseosa; Ovino; Pseudotuberculose; Sanidade animal. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/38390/1/v28n10a07.pdf
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Marc: |
LEADER 02603naa a2200349 a 4500
001 1326874
005 2011-07-22
008 2008 bl uuuu u00u1 u #d
100 1 $aABREU, S. R. de O.
245 $aComparação genotípica de isolados de Corynebacterium pseudotuberculosis de caprinos e ovinos do sertão de Pernambuco.
260 $c2008
520 $aResumo: Objetivou-se com este estudo comparar genotipicamente 35 isolados de Corynebacterium pseudotuberculosis recuperados de conteúdo de abscessos de caprinos e ovinos com linfadenite caseosa, procedentes de cinco municípios localizados no Sertão de Pernambuco, Brasil. Utilizou-se a técnica de fingerprint RFLP-PCR com as enzimas de restrição Hpy-Ch4 e Msp1 aplicada ao gene rpoB e as enzimas Pst I e Msp I para o gene pld. Não houve diferença nos padrões de fragmentos de bandas entre os isolados, independente da espécie hospedeira ou da área geográfica estudada, definindo-se um padrão genotípico homogêneo de C. pseudotuberculosis responsável por abscessos superficiais na região. [Genotypic comparison between Corynebacterium pseudotuberculosis samples obtained from sheep and goats with caseous lymphadenitis, raised in the semi-arid region of Pernambuco]. Abstract: The objective was to genotypically compare 35 samples of Corynebacterium pseudotuberculosis obtained from abscesses of sheep and goats diagnosed with caseous lymphadenitis originated from 5 different municipalities in the semi-arid region of Pernambuco, Brazil. The RFLP-PCR technique with Hpy-Ch4 and Msp I and Pst I Msp I restriction enzimes was used to fingerprint the genes rpoB and pld, respectively. The results demonstrate that there was no difference on the fragments banding pattern among samples, independently of the host species or geographic area studied, defining a homogeneous profile of C. pseudotuberculosis responsible for superficial abscesses for the region.
650 $aCaprino
650 $aCorynebacterium pseudotuberculosis
650 $aEpidemiologia
650 $aLinfadenite caseosa
650 $aOvino
650 $aPseudotuberculose
650 $aSanidade animal
653 $aPernambuco
653 $aRFLP-PCR
700 1 $aMOTA, R. A.
700 1 $aROSINHA, G. M. S.
700 1 $aFORNER, O.
700 1 $aPINHEIRO JÚNIOR, J. W.
700 1 $aPEREIRA, R. R. B.
700 1 $aCASTRO, R. S. de
700 1 $aELISEI, C.
700 1 $aSOARES, C. O.
700 1 $aARAÚJO, F. R.
700 1 $aMADUREIRA, R. C.
773 $tPesquisa Veterinária Brasileira, Seropédica$gv. 28, n. 10, p. 481-487, out. 2008.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
28/09/2015 |
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Data da última atualização: |
19/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BHERING, L. L.; JUNQUEIRA, V. S.; CRUZ, C. D.; LAVIOLA, B. G. |
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Afiliação: |
L.L. Bhering, Universidade Federal de Viçosa; V.S. Junqueira, Universidade Federal de Viçosa; C.D. Cruz, Universidade Federal de Viçosa; BRUNO GALVEAS LAVIOLA, CNPAE. |
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Título: |
Comparison of methods used to identify superior individuals in genomic selection in plant breeding. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research v. 14, n. 3, p. 10888-10896, 2015 |
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DOI: |
10.4238/2015 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate different methods used in genomic selection, and to verify those that select a higher proportion of individuals with superior genotypes. Thus, F2 populations of different sizes were simulated (100, 200, 500, and 1000 individuals) with 10 replications each. These consisted of 10 linkage groups (LG) of 100 cM each, containing 100 equally spaced markers per linkage group, of which 200 controlled the characteristics, defined as the 20 initials of each LG. Genetic and phenotypic values were simulated assuming binomial distribution of effects for each LG, and the absence of dominance. For phenotypic values, heritabilities of 20, 50, and 80% were considered. To compare methodologies, the analysis processing time, coefficient of coincidence (selection of 5, 10, and 20% of superior individuals), and Spearman correlation between true genetic values, and the genomic values predicted by each methodology were determined. Considering the processing time, the three methodologies were statistically different, rrBLUP was the fastest, and Bayesian LASSO was the slowest. Spearman correlation revealed that the rrBLUP and GBLUP methodologies were equivalent, and Bayesian LASSO provided the lowest correlation values. Similar results were obtained in coincidence variables among the individuals selected, in which Bayesian LASSO differed statistically and presented a lower value than the other methodologies. Therefore, for the scenarios evaluated, rrBLUP is the best methodology for the selection of genetically superior individuals. MenosThe aim of this study was to evaluate different methods used in genomic selection, and to verify those that select a higher proportion of individuals with superior genotypes. Thus, F2 populations of different sizes were simulated (100, 200, 500, and 1000 individuals) with 10 replications each. These consisted of 10 linkage groups (LG) of 100 cM each, containing 100 equally spaced markers per linkage group, of which 200 controlled the characteristics, defined as the 20 initials of each LG. Genetic and phenotypic values were simulated assuming binomial distribution of effects for each LG, and the absence of dominance. For phenotypic values, heritabilities of 20, 50, and 80% were considered. To compare methodologies, the analysis processing time, coefficient of coincidence (selection of 5, 10, and 20% of superior individuals), and Spearman correlation between true genetic values, and the genomic values predicted by each methodology were determined. Considering the processing time, the three methodologies were statistically different, rrBLUP was the fastest, and Bayesian LASSO was the slowest. Spearman correlation revealed that the rrBLUP and GBLUP methodologies were equivalent, and Bayesian LASSO provided the lowest correlation values. Similar results were obtained in coincidence variables among the individuals selected, in which Bayesian LASSO differed statistically and presented a lower value than the other methodologies. Therefore, for the scenarios evaluated, rrBLUP is the ... Mostrar Tudo |
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Palavras-Chave: |
Biomátrico; Biometric; Selection; SNPs. |
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Thesagro: |
Estatística; Seleção. |
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Thesaurus NAL: |
statistics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02292naa a2200253 a 4500
001 2025215
005 2017-09-19
008 2015 bl uuuu u00u1 u #d
024 7 $a10.4238/2015$2DOI
100 1 $aBHERING, L. L.
245 $aComparison of methods used to identify superior individuals in genomic selection in plant breeding.$h[electronic resource]
260 $c2015
520 $aThe aim of this study was to evaluate different methods used in genomic selection, and to verify those that select a higher proportion of individuals with superior genotypes. Thus, F2 populations of different sizes were simulated (100, 200, 500, and 1000 individuals) with 10 replications each. These consisted of 10 linkage groups (LG) of 100 cM each, containing 100 equally spaced markers per linkage group, of which 200 controlled the characteristics, defined as the 20 initials of each LG. Genetic and phenotypic values were simulated assuming binomial distribution of effects for each LG, and the absence of dominance. For phenotypic values, heritabilities of 20, 50, and 80% were considered. To compare methodologies, the analysis processing time, coefficient of coincidence (selection of 5, 10, and 20% of superior individuals), and Spearman correlation between true genetic values, and the genomic values predicted by each methodology were determined. Considering the processing time, the three methodologies were statistically different, rrBLUP was the fastest, and Bayesian LASSO was the slowest. Spearman correlation revealed that the rrBLUP and GBLUP methodologies were equivalent, and Bayesian LASSO provided the lowest correlation values. Similar results were obtained in coincidence variables among the individuals selected, in which Bayesian LASSO differed statistically and presented a lower value than the other methodologies. Therefore, for the scenarios evaluated, rrBLUP is the best methodology for the selection of genetically superior individuals.
650 $astatistics
650 $aEstatística
650 $aSeleção
653 $aBiomátrico
653 $aBiometric
653 $aSelection
653 $aSNPs
700 1 $aJUNQUEIRA, V. S.
700 1 $aCRUZ, C. D.
700 1 $aLAVIOLA, B. G.
773 $tGenetics and Molecular Research$gv. 14, n. 3, p. 10888-10896, 2015
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Embrapa Agroenergia (CNPAE) |
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