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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
21/03/2005 |
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Data da última atualização: |
06/05/2022 |
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Autoria: |
CASTRO, C. S. P. de; SOUZADE, J. R.; BLOCH JUNIOR, C. |
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Título: |
Mechanism of DNA cleavage catalyzed by mung bean nuclease. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Inorganica Chimica Acta, v. 357, n. 9, p. 2579-2592, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The interaction of zinc with different forms of DNA ( phage DNA, ss-oligo, ds-oligo) and Mung Bean Nuclease was studied by voltammetric techniques in order to investigate the mechanism of DNA cleavage catalyzed by a zinc metalloenzyme. Stoichiometry, dissociation constant, zinc binding sites and functions were determined for these systems. Two zinc ions were found to be involved in stabilization of a 19 mer ds-oligodeoxyribonucleotide, which was synthesized by the phosphoramidite method and used as a DNA model in the studies. Three zinc ions (Zn1, Zn2, and Zn3), which have different roles in ds-oligo cleavage, were identified in the active site of Mung Bean Nuclease. A concerted SN2 mechanism, which assigns a catalytic function to Zn2 and structural functions to Zn1 and Zn3, was proposed. The hydrolysis of phosphodiester bonds proceeds with inversion of configuration at the phosphorus center, forming a pentacoordinate transition state, which is stabilized by an arginine. Zn2 supplies the nucleophile, which is oriented by an aspartic acid, and activates the ds-oligo by its coordination to the phosphate free oxygen of the phosphodiester bond. Zn1 and Zn3 ions, besides stabilizing the tertiary structure of Mung Bean Nuclease, bind to the leaving group, blocking the cleavage reverse reaction. |
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Palavras-Chave: |
Clivagem; Zinco Nuclease mung bean. |
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Thesagro: |
DNA; Zinco. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01850naa a2200193 a 4500
001 1185564
005 2022-05-06
008 2004 bl uuuu u00u1 u #d
100 1 $aCASTRO, C. S. P. de
245 $aMechanism of DNA cleavage catalyzed by mung bean nuclease.$h[electronic resource]
260 $c2004
520 $aThe interaction of zinc with different forms of DNA ( phage DNA, ss-oligo, ds-oligo) and Mung Bean Nuclease was studied by voltammetric techniques in order to investigate the mechanism of DNA cleavage catalyzed by a zinc metalloenzyme. Stoichiometry, dissociation constant, zinc binding sites and functions were determined for these systems. Two zinc ions were found to be involved in stabilization of a 19 mer ds-oligodeoxyribonucleotide, which was synthesized by the phosphoramidite method and used as a DNA model in the studies. Three zinc ions (Zn1, Zn2, and Zn3), which have different roles in ds-oligo cleavage, were identified in the active site of Mung Bean Nuclease. A concerted SN2 mechanism, which assigns a catalytic function to Zn2 and structural functions to Zn1 and Zn3, was proposed. The hydrolysis of phosphodiester bonds proceeds with inversion of configuration at the phosphorus center, forming a pentacoordinate transition state, which is stabilized by an arginine. Zn2 supplies the nucleophile, which is oriented by an aspartic acid, and activates the ds-oligo by its coordination to the phosphate free oxygen of the phosphodiester bond. Zn1 and Zn3 ions, besides stabilizing the tertiary structure of Mung Bean Nuclease, bind to the leaving group, blocking the cleavage reverse reaction.
650 $aDNA
650 $aZinco
653 $aClivagem
653 $aZinco Nuclease mung bean
700 1 $aSOUZADE, J. R.
700 1 $aBLOCH JUNIOR, C.
773 $tInorganica Chimica Acta$gv. 357, n. 9, p. 2579-2592, 2004.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 11. |  | CASTRO, C. S. P. de; ARRUDA, A. F.; SCHWARTZ, E. N. Alcalóides de anfíbios: identificação de PTX, HTX, TRC e DHQ na pele de Dendrobates galactonotus por RP-HPLC e ESI-MS/MS. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE QUÍMICA, 28., 2005, Poços de Caldas, MG. Livro de resumos. São Paulo: Sociedade Brasileira de Química, 2005. Não paginado.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | TEIXEIRA, A. S.; CASTRO, A. C. R. de; CASTRO, C. S. P. de; LINHARES, H. P. Implementação de requisitos corporativos de qualidade no Banco Ativo de Germoplasma do Cajueiro da Embrapa. Revista RG News, v. 4, n. 3, p. 433, 2018 Resumos apresentado no CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 5., 2018, Fortaleza. Anais... Fortaleza: Sociedade Brasileira de Recursos Genéticos, 2018.| Tipo: Resumo em Anais de Congresso |
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| 18. | | OLIVEIRA FILHO, W. P. de; SOUZA, J. R. de; CASTRO, C. S. P. de. Estudo da interação entre a BSA e íons cobre (II) por voltametria de redissolução anódica. In: ENCONTRO NACIONAL DE QUÍMICA ANALÍTICA, 13.; CONGRESSO IBERO-AMERICANO DE QUÍMICA ANALÍTICA, 1., 2005, Niterói, RJ. [Anais...] Niterói: Universidade Federal Fluminense, Instituto de Química, 2005. Não paginado.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 143 | |
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