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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/11/2004 |
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Data da última atualização: |
06/05/2022 |
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Autoria: |
CASTRO, C. S. P. de; SOUZADE, J. R.; BLOCH JUNIOR, C. |
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Título: |
The binding of zinc (II) to a double-stranded oligodeoxyribonucleotide; a voltammetric study. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Biophysical Chemistry, v.112, n. 1, p. 59-67, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
Binding of zinc to a 19 mer double-stranded oligodeoxyribonucleotide was investigated by anodic stripping voltammetry and cyclic voltammetry in order to understand the roles of zinc in DNA cleavage catalyzed by mung bean nuclease. These methods rely on the direct monitoring of zinc oxidation current in the absence and in the presence of the oligo. Zinc titration curves with the ds-oligodeoxyribonucleotide were obtained in concentrations ranging from 3.62×10-9 to 3.62×10-8 M and 4.06×10-10 to 5.25×10-9 M. The acquired data were used to determine the dissociation constant, stoichiometry and zinc binding sites of the complex and to understand the specific changes of ds-oligodeoxyribonucleotide secondary structure by zinc binding. The oxidation-reduction process of zinc was also investigated by cyclic voltammetry through I (oxidation current) versus v1/2 (square root of scan rate) curves in the absence and in the presence of the double-stranded oligodeoxyribonucleotide. |
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Palavras-Chave: |
Metodos eletroquímicos; Voltametria. |
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Thesagro: |
DNA; Zinco. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177880/1/ID-24577.pdf
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Marc: |
LEADER 01555naa a2200193 a 4500
001 1185340
005 2022-05-06
008 2004 bl uuuu u00u1 u #d
100 1 $aCASTRO, C. S. P. de
245 $aThe binding of zinc (II) to a double-stranded oligodeoxyribonucleotide; a voltammetric study.$h[electronic resource]
260 $c2004
520 $aBinding of zinc to a 19 mer double-stranded oligodeoxyribonucleotide was investigated by anodic stripping voltammetry and cyclic voltammetry in order to understand the roles of zinc in DNA cleavage catalyzed by mung bean nuclease. These methods rely on the direct monitoring of zinc oxidation current in the absence and in the presence of the oligo. Zinc titration curves with the ds-oligodeoxyribonucleotide were obtained in concentrations ranging from 3.62×10-9 to 3.62×10-8 M and 4.06×10-10 to 5.25×10-9 M. The acquired data were used to determine the dissociation constant, stoichiometry and zinc binding sites of the complex and to understand the specific changes of ds-oligodeoxyribonucleotide secondary structure by zinc binding. The oxidation-reduction process of zinc was also investigated by cyclic voltammetry through I (oxidation current) versus v1/2 (square root of scan rate) curves in the absence and in the presence of the double-stranded oligodeoxyribonucleotide.
650 $aDNA
650 $aZinco
653 $aMetodos eletroquímicos
653 $aVoltametria
700 1 $aSOUZADE, J. R.
700 1 $aBLOCH JUNIOR, C.
773 $tBiophysical Chemistry$gv.112, n. 1, p. 59-67, 2004.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
19/08/2005 |
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Data da última atualização: |
07/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RANGEL, P. H. N.; BRONDANI, C.; RANGEL, P. N.; BRONDANI, R. P. V.; ZIMMERMANN, F. J. P. |
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Afiliação: |
PAULO HIDEO NAKANO RANGEL, CNPAF; CLAUDIO BRONDANI, CNPAF; PRISCILA NASCIMENTO RANGEL, bolsista CNPAF; ROSANA PEREIRA VIANELLO BRONDANI, CNPAF; FRANCISCO JOSÉ P. ZIMMERMANN. |
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Título: |
Development of rice lines with gene introgression from the wild Oryza glumaepatula by the AB-QTL methodology. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 5, n. 1, p. 10-19, Mar. 2005. |
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Idioma: |
Inglês |
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Conteúdo: |
Wild rice of the species Oryza glumaepatula is found Brazil and has been used to broaden the genetic basis of irrigated rice populations in Embrapa breeding programmes. The objective of this study was to demonstrate and discuss approaches used in the development of Oryza sativa lines containing genes transferred from Oryza glumaepatula, resulting in introgression lines with a broader genetic basis and high yield. First of all, genes were transferred from the wild species to cultivated rice by the AB-QTL methodology. Eighteen families were selected using QTL analysis and agronomical performance data. After the heterosis test, the families CNAi 9020 and CNAi 9024 were selected and submitted to microsatellite marker-assisted selection. Thirty-five lines were then selected with high plant vigour, high tiller and panicle number per plant, high grain yield of the main crop, and a strong regrowth capacity which makes the use of ratoons a feasible alternative. |
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Palavras-Chave: |
Irrigated rice; Lines; Linhagens; Marcadores microssatélites; Microsatellite markers; Produtividade de grãos. |
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Thesagro: |
Arroz Irrigado; Marcador Molecular; Oryza Sativa. |
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Thesaurus NAL: |
grain yield. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/24018/1/bd6b8337-3d8b-6f6c.pdf
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Marc: |
LEADER 01831naa a2200289 a 4500
001 1213573
005 2022-04-07
008 2005 bl uuuu u00u1 u #d
100 1 $aRANGEL, P. H. N.
245 $aDevelopment of rice lines with gene introgression from the wild Oryza glumaepatula by the AB-QTL methodology.
260 $c2005
520 $aWild rice of the species Oryza glumaepatula is found Brazil and has been used to broaden the genetic basis of irrigated rice populations in Embrapa breeding programmes. The objective of this study was to demonstrate and discuss approaches used in the development of Oryza sativa lines containing genes transferred from Oryza glumaepatula, resulting in introgression lines with a broader genetic basis and high yield. First of all, genes were transferred from the wild species to cultivated rice by the AB-QTL methodology. Eighteen families were selected using QTL analysis and agronomical performance data. After the heterosis test, the families CNAi 9020 and CNAi 9024 were selected and submitted to microsatellite marker-assisted selection. Thirty-five lines were then selected with high plant vigour, high tiller and panicle number per plant, high grain yield of the main crop, and a strong regrowth capacity which makes the use of ratoons a feasible alternative.
650 $agrain yield
650 $aArroz Irrigado
650 $aMarcador Molecular
650 $aOryza Sativa
653 $aIrrigated rice
653 $aLines
653 $aLinhagens
653 $aMarcadores microssatélites
653 $aMicrosatellite markers
653 $aProdutividade de grãos
700 1 $aBRONDANI, C.
700 1 $aRANGEL, P. N.
700 1 $aBRONDANI, R. P. V.
700 1 $aZIMMERMANN, F. J. P.
773 $tCrop Breeding and Applied Biotechnology$gv. 5, n. 1, p. 10-19, Mar. 2005.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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