Data/hora: 06/06/2024 03:33:03

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	05/10/1995	
Data da última atualização: 	05/10/1995	
Autoria: 	CASTRIC, P. A.; CONN, E. E.	
Afiliação: 	Department of Biochemistry and Biophysics, University of California, Davis, California.	
Título: 	Formation of b-cyanoalanine by o-acetylserine sulfhydrylase.	
Ano de publicação: 	1971	
Fonte/Imprenta: 	Journal of Bacteriology, v.108, n.1, p.132-136, 1971.	
Idioma: 	Inglês	
Conteúdo: 	Cell-free extracts of bacillus megaterium form B-cyanolanine (B-CNA)-14C from Na14CN and L-cysteine, O-acetyl-serine or, to a lesser extent, L-serine. However, the presence of cyanide in the growth medium does not increase the capacity of cell extracts to catalyze the formation of B-CNA from cysteine and cyanide. The formation of B-CNA is readily detected in extracts of cells grown in synthetic media with sulfate or L-djenkolic acid as souces: such cells also exhibit an increased ability to from cysteine when compared with cells grown on cysteine as the sulfur source. B-CNA formation could not be detected in extracts of cells grown on cysteine as the sulfur source. A 40-fold purification on the O-acetylsering sulfhydrylase resulted in the co-purification of the B-CNA- forming activity.          	
Palavras-Chave: 	HCN; Purificacao de enzinas.	
Thesagro: 	Purificação.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (16343-1) - ADC - SP - -- - S197 -  - S297 - DIS