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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
17/06/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D. |
Afiliação: |
Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. |
Título: |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008. |
DOI: |
10.1590/S1982-56762008000100010 |
Idioma: |
Inglês |
Conteúdo: |
The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. MenosThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. T... Mostrar Tudo |
Palavras-Chave: |
Doença bacteriana do milho; doenças foliares do milho; Maize bacterial disease; Maize leaf disease. |
Thesagro: |
Milho; Zea Mays. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32277/1/Localization-pantoea.pdf
|
Marc: |
LEADER 02604naa a2200277 a 4500 001 1491172 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 024 7 $a10.1590/S1982-56762008000100010$2DOI 100 1 $aBOMFETI, C. A. 245 $aLocalization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.$h[electronic resource] 260 $c2008 520 $aThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. 650 $aMilho 650 $aZea Mays 653 $aDoença bacteriana do milho 653 $adoenças foliares do milho 653 $aMaize bacterial disease 653 $aMaize leaf disease 700 1 $aSOUZA-PACCOLA, E. A. 700 1 $aMASSOLA JUNIOR, N. S. 700 1 $aMARRIEL, I. E. 700 1 $aMEIRELLES, W. F. 700 1 $aCASELA, C. R. 700 1 $aPACCOLA-MEIRELLES, L. D. 773 $tTropical Plant Pathology, Brasília$gv. 33, n. 1, p. 63-66, Jan./Feb. 2008.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
29/10/1998 |
Data da última atualização: |
21/07/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MAGALHAES, J. V. de; ALVES, V. M. C.; NOVAIS, R. F. de; MOSQUIM, P. R.; MAGALHAES, J. R.; BAHIA FILHO, A. F. C.; HUBER, D. M. |
Afiliação: |
JURANDIR VIEIRA DE MAGALHAES, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS. |
Título: |
Nitrate uptake by corn under increasing periods of phosphorus starvation. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Journal of Plant Nutrition, New York, v.21, n.8, p. 1753-1763, 1998. |
Idioma: |
Inglês |
Conteúdo: |
Single cross seedlings of the male progenitor of the double cross hybrid, BR 201, were grown for seven days in a complete nutrient solution prior in evaluating the kinetics of nitrate uptake under varying periods of phosphorus deficiency. Nitrate uptake decreased 60% after a short period of phosphorus starvation (2 days) and ceased when phosphorus was withheld longer than six days. Nitrate uptake resumed after phosphorus (P) was re-supplied, but the time required and extent of recovery depended on the length of phosphorus starvation. |
Palavras-Chave: |
Maize; Nitrate. |
Thesagro: |
Fósforo; Milho; Nitrato; Zea Mays. |
Thesaurus NAL: |
phosphorus; starvation. |
Categoria do assunto: |
W Química e Física |
Marc: |
LEADER 01325naa a2200289 a 4500 001 1480738 005 2018-07-21 008 1998 bl uuuu u00u1 u #d 100 1 $aMAGALHAES, J. V. de 245 $aNitrate uptake by corn under increasing periods of phosphorus starvation.$h[electronic resource] 260 $c1998 520 $aSingle cross seedlings of the male progenitor of the double cross hybrid, BR 201, were grown for seven days in a complete nutrient solution prior in evaluating the kinetics of nitrate uptake under varying periods of phosphorus deficiency. Nitrate uptake decreased 60% after a short period of phosphorus starvation (2 days) and ceased when phosphorus was withheld longer than six days. Nitrate uptake resumed after phosphorus (P) was re-supplied, but the time required and extent of recovery depended on the length of phosphorus starvation. 650 $aphosphorus 650 $astarvation 650 $aFósforo 650 $aMilho 650 $aNitrato 650 $aZea Mays 653 $aMaize 653 $aNitrate 700 1 $aALVES, V. M. C. 700 1 $aNOVAIS, R. F. de 700 1 $aMOSQUIM, P. R. 700 1 $aMAGALHAES, J. R. 700 1 $aBAHIA FILHO, A. F. C. 700 1 $aHUBER, D. M. 773 $tJournal of Plant Nutrition, New York$gv.21, n.8, p. 1753-1763, 1998.
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