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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/11/2017 |
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Data da última atualização: |
06/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, M. J. S.; OLIVEIRA, E. J. de; SOUZA, A. da S.; PEREIRA, J. S.; DIAMANTINO, M. S. A. S.; OLIVEIRA, S. A. S. de. |
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Afiliação: |
M. J. S. CARVALHO; EDER JORGE DE OLIVEIRA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; J. S. PEREIRA; M. S .A. S. DIAMANTINO; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF. |
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Título: |
Cleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 16, n.2, 2017. |
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Série: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease. MenosThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic ro... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
cassava. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02559naa a2200217 a 4500
001 2078909
005 2017-11-06
008 2017 bl --- 0-- u #d
100 1 $aCARVALHO, M. J. S.
245 $aCleaning cassava genotypes infected with cassava frogskin disease via in vitro shoot tip culture.$h[electronic resource]
260 $c2017
490 $a1676-5680
520 $aThis study aimed to develop a methodology for eliminating cassava frogskin disease (CFSD) from in vitro shoot tip culture by associating thermotherapy and tetracycline. Cuttings from different accessions (BGM0232, BGM0315, BGM0464, BGM584, BGM0841, and BGM1342), infected with CFSD according to visual inspection of the disease symptoms, were used for cleaning. To verify the absence of other diseases, the plants were indexed for Cassava common mosaic virus - CsCMV (by ELISA) and Cassava vein mosaic virus - CsVMV (by polymerase chain reaction, PCR), proving that the accessions were free of these viruses, except for BGM0315 and BGM0464, which were infected with CsVMV. Subsequently, the cuttings were submitted to different tetracycline concentrations for 3 min, and then subjected to thermotherapy under different temperatures (35°, 38°, 40°, 45°, and 55°C). Shoots of 2 cm were harvested, and their surfaces were sterilized in a laminar flow chamber. Subsequently, the shoot tips of different sizes were removed (0.2, 0.4, 0.5, and 1.0 mm) for inoculation in a culture medium with tetracycline at the same concentrations in which the cuttings were dipped. After 60 days of cultivation, the explants were transferred to a multiplication medium without antibiotics. Thirty days after the transfer, the viability of the regenerated plants was evaluated, which were then acclimatized for 70 days in a greenhouse and transferred to the field. After 7 months, a visual analysis of the symptomatic roots and a PCR analysis were held to prove the elimination of CFSD and CsVMV from the accessions infected with these viruses (BGM0315 and BGM0464), respectively. Most of the treatments resulted in 100% cleaning of CFSD-infected plants. From accessions that were also infected with CsVMV, only 2% of the plants remained infected, also demonstrating the cleaning efficiency of this protocol for this disease.
650 $acassava
650 $aMandioca
700 1 $aOLIVEIRA, E. J. de
700 1 $aSOUZA, A. da S.
700 1 $aPEREIRA, J. S.
700 1 $aDIAMANTINO, M. S. A. S.
700 1 $aOLIVEIRA, S. A. S. de
773 $tGenetics and Molecular Research$gv. 16, n.2, 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 58 | |
| 21. |  | CRISPIM, S. M. A.; SANTOS, S. A.; SORIANO, B. M. A.; DOMINGOS BRANCO, O. Descrição de duas fitofisionomias e sua propensão aos incêndios no Pantanal, MS, Brasil. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 46., Maringá, 2009. Maringá: UEM, 2009. 3 p. Título em inglês: Description of two fitofisionomias and propensity to fire the Pantanal, MS, Brazil.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pantanal. |
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| 22. | | CRISPIM, S. M. A.; ABREU, U. G. P. de; SORIANO, B. M. A.; DOMINGOS BRANCO, O. Determinação de nutrientes, fibra e lignina em Brachiaria decumbers, Pantanal, Corumbá, Brasil = Determination of nutrients, fiber and lignin in Brachiaria decumbers, Pantanal, Corumbá, Brazil. In: REUNION ASOCIACION LATINOAMERICANA DE PRODUCCION ANIMAL, 20.; REUNION ASOCIACION PERUANA DE PRODUCCION ANIMAL, 30.; CONGRESO INTERNACIONAL DE GANADERIA, 5. Cusco, 2007. Anais...Cusco: APPA, 2007. 4 p. Del 21 al 25 de octubre.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pantanal. |
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| 25. | | CRISPIM, S. M. A.; SANTOS, S. A.; BARIONI JUNIOR, W.; BRANCO, O. D. Variacao sazonal na producao de materia seca e composicao botanica em area de pastagem nativa, sob pastejo, Pantanal - MS. In: REUNIAO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 41., 2004, Campo Grande-MS. A producao anual e a seguranca alimentar - anais. Campo Grande, MS: SBZ, 2004. CD ROM 5 p.| Biblioteca(s): Embrapa Pantanal. |
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| 32. | | CRISPIM, S. M. A.; SORIANO, B. M. A.; BRANCO, O. D. Regeneracao de especies herbaceas nativas pos-queima em area de reserva, sub-regiao da Nhecolandia, Pantanal, MS. In: REUNIAO ANIAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 40., Santa Maria, 2003. Resumos... Santa Maria: SBZ, 2003. p.1-6.| Biblioteca(s): Embrapa Pantanal. |
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| 35. | | CARDOSO, E. L.; CRISPIM, S. M. A.; RODRIGUES, C. A. G.; BARIONI JUNIOR, W.; BRANCO, O. D. Avaliacao da composicao quimica do "caronal" com e sem queima no Pantanal, MS. In: SIMPOSIO SOBRE RECURSOS NATURAIS E SOCIO-ECONOMICOS DO PANTANAL, 3., 2000, Corumba. Os desafios do novo milenio - resumos. Corumba: Embrapa Pantanal, 2000. p.175.| Biblioteca(s): Embrapa Pantanal. |
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| 36. | | SALLES, C. A. O.; CORÁ, T. C. L.; SALIS, S. M. de; URBANETZ, C.; DOMINGOS BRANCO, O. Fenologia da bocaiúva, safra 2015-2016, em Antônio Maria Coelho, Corumbá, MS. In: EVENTO DE INICIAÇÃO CIENTÍFICA DO PANTANAL, 4.; SEMANA DA BIOLOGIA, 11., 2016, Corumbá. Resumos... Corumbá: Embrapa Pantanal, 2016. p. 21. (Embrapa Pantanal. Documentos, 141).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 37. | | PADILHA, D. R. C.; SALIS, S. M.; ASSIS, E. L. M. de; LEHN, C. R.; BRANCO, O. D. Florística e fitossociologia de um cerradão no Pantanal da Nhecolândia, MS. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PANTANAL E UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL, 2.; 2008, Corumbá. Resumos...Corumbá: Embrapa Pantanal, 2008. (Embrapa Pantanal. Documentos, 95). 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 38. | | CRISPIM, S. M. A.; SANTOS, S. A.; CARDOSO, E. L.; BRANCO, O. D.; SORIANO, B. M. A. Efeito da queima e herbivoria em áreas de campo cerrado na Sub-região de Poconé, MT. In: SIMPÓSIO SOBRE RECURSOS NATURAIS E SÓCIO-ECONÔMICOS DO PANTANAL, 4., 2004, Corumbá, MS. Sustentabilidade regional: anais. Corumbá: Embrapa Pantanal: UCDB: UFMS: SEBRAE-MS, 2004. (CD-ROM).| Biblioteca(s): Embrapa Pantanal. |
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| 40. | | CRISPIM, S. M. A.; RODRIGUES, C. A. G.; BARIONI JÚNIOR, W.; SORIANO, B. M. A.; BRANCO, O. D. Disponibilidade de matéria seca nas diferentes fitofisionomias do Pantanal, sub-região da Nhecolândia, MS. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 42., Goiânia, 2005. Anais eletrônicos: A produção animal e o foco no agronegócio. Goiânia: SBZ, 2005. 6 p. 1 CD-ROM| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Territorial. |
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| Registros recuperados : 58 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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