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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
30/03/2016 |
Data da última atualização: |
30/03/2016 |
Autoria: |
CARVALHO, Y. K. P. de; ARGÔLO-NETO, N. M.; AMBRÓSIO, C. E.; OLIVEIRA, L. de J. de; ROCHA, A. R. da; SILVA, J. B. da; CARVALHO, M. A. M. de; ALVES, F. R. |
Afiliação: |
YULLA K. P. DE CARVALHO, UFPI; NAPOLEÃO M. ARGÔLO-NETO, UFPI; CARLOS E. AMBRÓSIO, USP; LILIAN DE JESUS DE OLIVEIRA, USP; ANDRESSA R. DA ROCHA, UFPI; JANETE B. DA SILVA, UFPI; MARIA ACELINA M. DE CARVALHO, UFPI; FLÁVIO R. ALVES, UFPI. |
Título: |
Isolation, expansion and differentiation of cellular progenitors obbtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831). |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 590-598, jun. 2015 |
Idioma: |
Inglês |
Conteúdo: |
The study aimed to isolate, expand, differentiate and characterize progenitor cells existent in the dental pulp of agouti. The material was washed with PBS solution and dissociated mechanically with the aid of a scalpel blade on plates containing culture medium D-MEM/F-12, and incubated at 5% CO2-37°C. The growth curve, CFU assay, osteogenic/ adipogenic differentiation and characterization were obtained from the isolation. The cells began to be released from the explant tissue around the 7th day of culture. By day 22 of culture, cells reached 80% confluence. At the UFC test, 81 colonies were counted with 12 days of cultivation. The growth curves before and after freezing showed a regular growth with intense proliferation and clonogenic potential. The cell differentiation showed formation of osteoblasts and fat in culture, starting at 15 days of culture in a specific medium. Flow cytometry (FACs) was as follows: CD34 (positive), CD14 (negative), CD45 (negative), CD73 (positive), CD79 (negative), CD90 (positive), CD105 (positive), demonstrating high specificity and commitment of isolated cells with mesenchymal stem cells strains. These results suggest the existence of a cell population of stem cells with mesenchymal features from the isolated tissue in the explants of agouti dental pulp, a potential model for study of stem cell strains obtained from the pulp tissue. |
Palavras-Chave: |
Biologia celular; Cultivo celular; Dasyprocta prymnolopha; Diferenciação celular; MSC da polpa dentária; MSC from dental pulp. |
Thesaurus Nal: |
Cell biology; Cell culture; Cell differentiation. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141856/1/Isolation-expansion.pdf
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Marc: |
LEADER 02395naa a2200313 a 4500 001 2042235 005 2016-03-30 008 2015 bl uuuu u00u1 u #d 100 1 $aCARVALHO, Y. K. P. de 245 $aIsolation, expansion and differentiation of cellular progenitors obbtained from dental pulp of agouti (Dasyprocta prymnolopha Wagler, 1831). 260 $c2015 520 $aThe study aimed to isolate, expand, differentiate and characterize progenitor cells existent in the dental pulp of agouti. The material was washed with PBS solution and dissociated mechanically with the aid of a scalpel blade on plates containing culture medium D-MEM/F-12, and incubated at 5% CO2-37°C. The growth curve, CFU assay, osteogenic/ adipogenic differentiation and characterization were obtained from the isolation. The cells began to be released from the explant tissue around the 7th day of culture. By day 22 of culture, cells reached 80% confluence. At the UFC test, 81 colonies were counted with 12 days of cultivation. The growth curves before and after freezing showed a regular growth with intense proliferation and clonogenic potential. The cell differentiation showed formation of osteoblasts and fat in culture, starting at 15 days of culture in a specific medium. Flow cytometry (FACs) was as follows: CD34 (positive), CD14 (negative), CD45 (negative), CD73 (positive), CD79 (negative), CD90 (positive), CD105 (positive), demonstrating high specificity and commitment of isolated cells with mesenchymal stem cells strains. These results suggest the existence of a cell population of stem cells with mesenchymal features from the isolated tissue in the explants of agouti dental pulp, a potential model for study of stem cell strains obtained from the pulp tissue. 650 $aCell biology 650 $aCell culture 650 $aCell differentiation 653 $aBiologia celular 653 $aCultivo celular 653 $aDasyprocta prymnolopha 653 $aDiferenciação celular 653 $aMSC da polpa dentária 653 $aMSC from dental pulp 700 1 $aARGÔLO-NETO, N. M. 700 1 $aAMBRÓSIO, C. E. 700 1 $aOLIVEIRA, L. de J. de 700 1 $aROCHA, A. R. da 700 1 $aSILVA, J. B. da 700 1 $aCARVALHO, M. A. M. de 700 1 $aALVES, F. R. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 6, p. 590-598, jun. 2015
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Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
09/09/2005 |
Data da última atualização: |
18/05/2022 |
Autoria: |
PRAÇA, L. B.; DUMAS, V. F.; RAMOS, F. R.; MARTINS, E. S.; MONNERAT, R. G. |
Título: |
Caracterização entomopatogênica, bioquímica e molecular de estirpes de Bacillus thuringiensis tóxicas ao bicudo do algodoeiro (Anthonomus grandis Boheman, 1843). |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ALGODÃO, 5., 2005, Salvador. Algodão: uma fibra natural: resumos. Salvador: ABAPA, 2005. |
Páginas: |
Não paginado. |
Idioma: |
Português |
Palavras-Chave: |
Estirpes. |
Thesagro: |
Anthonomus Grandis; Bacillus Thuringiensis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178451/1/ID-25562.pdf
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Marc: |
LEADER 00743nam a2200193 a 4500 001 1186109 005 2022-05-18 008 2005 bl uuuu u00u1 u #d 100 1 $aPRAÇA, L. B. 245 $aCaracterização entomopatogênica, bioquímica e molecular de estirpes de Bacillus thuringiensis tóxicas ao bicudo do algodoeiro (Anthonomus grandis Boheman, 1843).$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE ALGODÃO, 5., 2005, Salvador. Algodão: uma fibra natural: resumos. Salvador: ABAPA$c2005 300 $aNão paginado. 650 $aAnthonomus Grandis 650 $aBacillus Thuringiensis 653 $aEstirpes 700 1 $aDUMAS, V. F. 700 1 $aRAMOS, F. R. 700 1 $aMARTINS, E. S. 700 1 $aMONNERAT, R. G.
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