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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
15/04/2004 |
Data da última atualização: |
15/04/2004 |
Autoria: |
CABRAL, G. B; CARVALHO, L. J. C. B; SCHAAL, B. A. |
Título: |
The formation of the storage root in cassava: an anatomical and A 2-D protein analysis. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17 p.61, nov., 1998. Suplemento. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Store root (SR) formation in cassava is an important trait related to starch formation and quality. It is the result of secondary growth and starch deposition in parenchyma cells. No distinct developmental stage for this organ can be established based on root morphology ony because of indeterminate SR growth nature and the continuous cambium activities which give raises to the root diameter. In order to understand cassava store root development our research is focusing on the pattern of tissue differentiation during the raising of diameter. Store root morphology (root diameter), anatomy and deferential gene expression are being studied. Changing in root diameter was, first used to differentiate stages of SR formation. Anatomical observation with different tissue stains, revealed the same tissue pattern in five SR diameter, except the fibrous root. Cambium activity and radial dislocation were observed as SR were being formed. Proportion of different specialized cell layers such as store parenchyma, cabium and cortex varied with the raise of SR diameter. An SDS-PAGE analysis of protein extracted from SR of five different diameter indicated a simple protein pattern and a differential pattern between fibrou's root and store root. Later SR stage also showed particular pattern differing from the early ones. High resolution 2-D gel electrophoresis analysis separated several classes of proteins related to SR diameter. These results are discussed in relation to the store root anatomical changes during the secondary growth. MenosAbstract: Store root (SR) formation in cassava is an important trait related to starch formation and quality. It is the result of secondary growth and starch deposition in parenchyma cells. No distinct developmental stage for this organ can be established based on root morphology ony because of indeterminate SR growth nature and the continuous cambium activities which give raises to the root diameter. In order to understand cassava store root development our research is focusing on the pattern of tissue differentiation during the raising of diameter. Store root morphology (root diameter), anatomy and deferential gene expression are being studied. Changing in root diameter was, first used to differentiate stages of SR formation. Anatomical observation with different tissue stains, revealed the same tissue pattern in five SR diameter, except the fibrous root. Cambium activity and radial dislocation were observed as SR were being formed. Proportion of different specialized cell layers such as store parenchyma, cabium and cortex varied with the raise of SR diameter. An SDS-PAGE analysis of protein extracted from SR of five different diameter indicated a simple protein pattern and a differential pattern between fibrou's root and store root. Later SR stage also showed particular pattern differing from the early ones. High resolution 2-D gel electrophoresis analysis separated several classes of proteins related to SR diameter. These results are discussed in relation to the store r... Mostrar Tudo |
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LEADER 02010naa a2200145 a 4500 001 1651911 005 2004-04-15 008 1998 bl uuuu u00u1 u #d 100 1 $aCABRAL, G. B 245 $aThe formation of the storage root in cassava$ban anatomical and A 2-D protein analysis. 260 $c1998 520 $aAbstract: Store root (SR) formation in cassava is an important trait related to starch formation and quality. It is the result of secondary growth and starch deposition in parenchyma cells. No distinct developmental stage for this organ can be established based on root morphology ony because of indeterminate SR growth nature and the continuous cambium activities which give raises to the root diameter. In order to understand cassava store root development our research is focusing on the pattern of tissue differentiation during the raising of diameter. Store root morphology (root diameter), anatomy and deferential gene expression are being studied. Changing in root diameter was, first used to differentiate stages of SR formation. Anatomical observation with different tissue stains, revealed the same tissue pattern in five SR diameter, except the fibrous root. Cambium activity and radial dislocation were observed as SR were being formed. Proportion of different specialized cell layers such as store parenchyma, cabium and cortex varied with the raise of SR diameter. An SDS-PAGE analysis of protein extracted from SR of five different diameter indicated a simple protein pattern and a differential pattern between fibrou's root and store root. Later SR stage also showed particular pattern differing from the early ones. High resolution 2-D gel electrophoresis analysis separated several classes of proteins related to SR diameter. These results are discussed in relation to the store root anatomical changes during the secondary growth. 700 1 $aCARVALHO, L. J. C. B 700 1 $aSCHAAL, B. A. 773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17 p.61, nov., 1998. Suplemento.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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3. | | CARVALHO, L. J. C. B. Book reviews: Guarino, L.; Rao, V.R.; Reid, R., eds. Collecting plant genetic diversity: technical guidelines. Plant Science Bulletin, Saint Louis, v.42, n.2, p.42, 1996. ResumoBiblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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5. | | CARVALHO, L. J. C. B. The storage roots of cassava. In: Collaborative crop researc : an invited symposium 27-29 Nov.2000, San Diego, CA. Brasília: Embrapa genetic Resources and biotechnology, 2000. ( Embrapa Genetic Resources and Biotechnology. Documentos, 52) 1 CD-ROM.Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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11. | | BUSO, G. S. C.; CARVALHO, L. J. C. B.; NASSAR, N. M. A. Analysis of Genetic relationships amongs Manihot interspecific hybrids and their parental species, using RAPD assay. In: THE CASSAVA biotechnology network: proceedings of the second international scientific meeting, Bogor, Indonesia, 22-26 August 1994. Cali: CIAT, 1995, V.1, p.101-105. (CIAT. Working Document, n.150). p.101-105 (CIAT. Working Document, n.150).Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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13. | | DJABOU, A. S. M.; CARVALHO, L. J. C. B.; LI, Q. S.; NIEMENAK, N.; CHEN, S. Cassava postharvest physiological deterioration: a complex phenomenon involving calcium signaling, reactive oxygen species and programmed cell death. Acta Physiologiae Plantarum, v. 39, n. 4, article 91, 2017.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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19. | | VALLE, A. M. A.; CARVALHO, L. J. C. B.; CASCARDO, J. C. M.; SOUZA, C. B. Diversidade bioquímica e genética de síntese de carotenóides em raiz de reserva de mandioca (Manihot esculenta Crantz). In: CONGRESSO BRASILEIRO DE GENÉTICA, 52., 2006, Foz do Iguaçu, PR. Resumos... Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2006. p. 947.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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