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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
18/08/1999 |
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Data da última atualização: |
20/02/2023 |
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Autoria: |
CARVALHO, C. H. S. |
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Afiliação: |
CARLOS H. S. CARVALHO, EMBRAPA-CNPMS. |
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Título: |
Agrobacterium-mediated transformation of sorghum and analysis of putative transposable element-induced mutants in sorghum. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
1999. |
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Páginas: |
99 p. |
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Idioma: |
Inglês |
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Notas: |
Tese (Doutorado) - Purdue University, West Lafayette. |
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Conteúdo: |
Plant biotechnology has become a powerful tool to complement the traditional methods of plants improvement. Several methodologies have been developed to identify and clone agronomically important genes to transfer genes from any living organism to plants. This work encompasses the study of two methodologies related to the identification and transferring of genes in sorghum. The first part reports the development of a protocol for sorghum transformation via Agrobacterium tumefaciens. It is demonstrated that Agrobacterium-mediated transformation is a feasible technique for the genetic transformation of sorghum. Sorghum transgenic plants were produced via Agrobacterium tumefaciens, and the transformation evidenced by Southern blot analysis of To and T1 plants, detection of GUS activity, and production of T1 plants resistant to hygromycin. Immature embryos of sorghum were very sensitive to Agrobacterium, and embryo death after co-cultivation was considered the limiting step to increase the transformation efficiency. Key factors were the co-cultivation medium, the use of a genotype and an explant with good tissue culture response, and the addition of Pluronic F-68 to the inoculation medium. Sorghum transformation via Agrobacterium is still not a routine technique, but it seems to have good potential once the protocol is further refined and improved. The second part of the thesis describes the analysis of several putative transposable element-induced mutants in sorghum identified in a Candystripe population, and the identification of endogenous transposable elements in sorghum derived from the cs1 family. Lastly, a likely involvement of a transposable element in the susceptibility of Colby to Periconia circinata is dicussed. MenosPlant biotechnology has become a powerful tool to complement the traditional methods of plants improvement. Several methodologies have been developed to identify and clone agronomically important genes to transfer genes from any living organism to plants. This work encompasses the study of two methodologies related to the identification and transferring of genes in sorghum. The first part reports the development of a protocol for sorghum transformation via Agrobacterium tumefaciens. It is demonstrated that Agrobacterium-mediated transformation is a feasible technique for the genetic transformation of sorghum. Sorghum transgenic plants were produced via Agrobacterium tumefaciens, and the transformation evidenced by Southern blot analysis of To and T1 plants, detection of GUS activity, and production of T1 plants resistant to hygromycin. Immature embryos of sorghum were very sensitive to Agrobacterium, and embryo death after co-cultivation was considered the limiting step to increase the transformation efficiency. Key factors were the co-cultivation medium, the use of a genotype and an explant with good tissue culture response, and the addition of Pluronic F-68 to the inoculation medium. Sorghum transformation via Agrobacterium is still not a routine technique, but it seems to have good potential once the protocol is further refined and improved. The second part of the thesis describes the analysis of several putative transposable element-induced mutants in sorghum identified ... Mostrar Tudo |
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Thesagro: |
Agrobacterium Tumefaciens; Biotecnologia; Sorghum Bicolor; Sorgo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02330nam a2200181 a 4500
001 1482512
005 2023-02-20
008 1999 bl uuuu m 00u1 u #d
100 1 $aCARVALHO, C. H. S.
245 $aAgrobacterium-mediated transformation of sorghum and analysis of putative transposable element-induced mutants in sorghum.$h[electronic resource]
260 $a1999.$c1999
300 $a99 p.
500 $aTese (Doutorado) - Purdue University, West Lafayette.
520 $aPlant biotechnology has become a powerful tool to complement the traditional methods of plants improvement. Several methodologies have been developed to identify and clone agronomically important genes to transfer genes from any living organism to plants. This work encompasses the study of two methodologies related to the identification and transferring of genes in sorghum. The first part reports the development of a protocol for sorghum transformation via Agrobacterium tumefaciens. It is demonstrated that Agrobacterium-mediated transformation is a feasible technique for the genetic transformation of sorghum. Sorghum transgenic plants were produced via Agrobacterium tumefaciens, and the transformation evidenced by Southern blot analysis of To and T1 plants, detection of GUS activity, and production of T1 plants resistant to hygromycin. Immature embryos of sorghum were very sensitive to Agrobacterium, and embryo death after co-cultivation was considered the limiting step to increase the transformation efficiency. Key factors were the co-cultivation medium, the use of a genotype and an explant with good tissue culture response, and the addition of Pluronic F-68 to the inoculation medium. Sorghum transformation via Agrobacterium is still not a routine technique, but it seems to have good potential once the protocol is further refined and improved. The second part of the thesis describes the analysis of several putative transposable element-induced mutants in sorghum identified in a Candystripe population, and the identification of endogenous transposable elements in sorghum derived from the cs1 family. Lastly, a likely involvement of a transposable element in the susceptibility of Colby to Periconia circinata is dicussed.
650 $aAgrobacterium Tumefaciens
650 $aBiotecnologia
650 $aSorghum Bicolor
650 $aSorgo
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Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 221 | |
| 13. |  | MARTINS, M. F.; VASCONCELOS, M. J. V.; CARVALHO, C. H. S.; PAIVA, E. Análise de RFLP em cultura de calos de milho (Zea mays L.) e suas plantas regeneradas. In: CONGRESSO NACIONAL DE MILHO E SORGO, 19.; REUNIÃO TÉCNICA ANUAL DE MILHO, 37.; REUNIÃO TÉCNICA ANUAL DO SORGO, 21., 1992, Porto Alegre. Resumos. Porto Alegre: SAA, 1992. p. 59.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 20. | | CARNEIRO, N. P.; CARNEIRO, A. A.; CARVALHO, C. H. S.; LOPES, M. A.; PAIVA, E. Caracterização genético-bioquímica de milho tropical transformado com o gene que codifica para a zeína. Genetics and Molecular Biology, Ribeirão Preto, v. 23, n. 3, p. 235-236, 2000. Suplemento. Edição dos resumos do 46º Congresso Nacional de Genética, Águas de Lindóia, SP, 2000.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 221 | |
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