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Registro Completo |
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
30/11/2012 |
Data da última atualização: |
05/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GOES, K. C. G. P. de; FISHER, M. L. de C.; CATTELAN, A. J.; NOGUEIRA, M. A.; CARVALHO, C. G. P. de; OLIVEIRA, A. L. M. de. |
Afiliação: |
KELLY CAMPOS GUERRA PINHEIRO DE GOES, UEL; MARIA LUISA DE CASTRO FISHER, UEL; ALEXANDRE JOSE CATTELAN, CNPSO; MARCO ANTONIO NOGUEIRA, CNPSO; CLAUDIO GUILHERME PORTELA CARVALHO, CNPSO; ANDRÉ LUIZ MARTINEZ DE OLIVEIRA, UEL. |
Título: |
Biochemical and molecular characterization of high population density bacteria isolated from sunflower. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Microbiology and Biotechnology, Seoul, v. 22, n. 4, p. 437-447, Feb. 2012. |
DOI: |
10.4014/jmb.1109.09007 |
Idioma: |
Inglês |
Conteúdo: |
Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods,successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B.cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillussp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed broad diversity among the Bacillus isolates,which clustered into 2 groups with 75% similarity and 13 subgroups with 85% similarity, suggesting that the genetic distance correlated with the source of isolation. The isolates weralso analyzed for certain growth-promoting activitiesAuxin synthesis was widely distributed among the isolates, with values ranging from 93.34 to 1653.37 µM auxin per µg of protein. The phosphate solubilization index ranged from 1.25 to 3.89, and siderophore index varied from 1.15 to 5.25. From a total of 57 isolates, 3 showed an ability to biologically fix atmospheric nitrogen, and 7 showed antagonism against the pathogen Sclerotinia sclerotiorumThe results of biochemical characterization allowed identification of potential candidates for the developmenof biofertilizers targeted to the sunflower crop. MenosNatural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the... 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Bactéria. |
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LEADER 05995naa a2200205 a 4500 001 1940892 005 2017-08-05 008 2012 bl uuuu u00u1 u #d 024 7 $a10.4014/jmb.1109.09007$2DOI 100 1 $aGOES, K. C. G. P. de 245 $aBiochemical and molecular characterization of high population density bacteria isolated from sunflower.$h[electronic resource] 260 $c2012 520 $aNatural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing:42 Bacillus (B. subtilis, B. cereus, B.thuringiensis, B.pumilus,B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods, successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B. cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillus sp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed a broad diversity among the Bacillus isolates, which clustered Natural and beneficial associations between plants and bacteria have demonstrated potential commercial application for several agricultural crops. The sunflower has acquired increasing importance in Brazilian agribusiness owing to its agronomic characteristics such as the tolerance to edaphoclimatic variations, resistance to pests and diseases, and adaptation to the implements commonly used for maize and soybean, as well as the versatility of the products and by-products obtained from its cultivation. A study of the cultivable bacteria associated with two sunflower cultivars, using classical microbiological methods,successfully obtained isolates from different plant tissues (roots, stems, florets, and rhizosphere). Out of 57 plantgrowth-promoting isolates obtained, 45 were identified at the genus level and phylogenetically positioned based on 16S rRNA gene sequencing: 42 Bacillus (B. subtilis, B.cereus, B. thuringiensis, B. pumilus, B. megaterium, and Bacillussp.) and 3 Methylobacterium komagatae. Random amplified polymorphic DNA (RAPD) analysis showed broad diversity among the Bacillus isolates,which clustered into 2 groups with 75% similarity and 13 subgroups with 85% similarity, suggesting that the genetic distance correlated with the source of isolation. The isolates weralso analyzed for certain growth-promoting activitiesAuxin synthesis was widely distributed among the isolates, with values ranging from 93.34 to 1653.37 µM auxin per µg of protein. The phosphate solubilization index ranged from 1.25 to 3.89, and siderophore index varied from 1.15 to 5.25. From a total of 57 isolates, 3 showed an ability to biologically fix atmospheric nitrogen, and 7 showed antagonism against the pathogen Sclerotinia sclerotiorumThe results of biochemical characterization allowed identification of potential candidates for the developmenof biofertilizers targeted to the sunflower crop. 650 $aBactéria 700 1 $aFISHER, M. L. de C. 700 1 $aCATTELAN, A. J. 700 1 $aNOGUEIRA, M. A. 700 1 $aCARVALHO, C. G. P. de 700 1 $aOLIVEIRA, A. L. M. de 773 $tJournal of Microbiology and Biotechnology, Seoul$gv. 22, n. 4, p. 437-447, Feb. 2012.
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Registros recuperados : 211 | |
83. | | OLIVEIRA, I. R. de; CARVALHO, HJ. W. L. de; LIRA, M. A. L.; CARVALHO, C. G. P. de; RIBEIRO, S. S.; OLIVEIRA, V. D. de. Avaliação de cultivares de girassol na zona agreste do nordeste brasileiro. In: REUNIÃO NACIONAL DE PRESQUISA DE GIRASSOL, 17., SIMPÓSIO NACIONAL SOBRE A CULTURA DE GIRASSOS, 5., 2007, Uberaba. Anais... Uberaba: Embrapa Soja, EPAMIG, 2007.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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84. | | OLIVEIRA, I. R. de; CARVALHO, H. W. L. de; LIRA, M. A.; CARVALHO, C. G. P. de; RIBEIRO, S. S.; OLIVEIRA, V. D. de. Avaliação de cultivares de girassol na Zona Agreste do Nordeste Brasileiro. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 17.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 5., 2007, Uberaba. Anais... Londrina: Embrapa Soja, 2007. p. 197-200. (Embrapa Soja. Documentos, 292). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campos Leite, Simone Ery Grosskopf.Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Soja. |
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89. | | AMABILE, R. F.; MONTEIRO, V. A.; AQUINO, F. D. V. de; CARVALHO, C. G. P. de; RIBEIRO JÚNIOR, W. Q.; FERNANDES, F. D.; SANTORO, V. de L. Avaliação de genótipos de girassol em safrinha no Cerrado do Distrito Federal. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 17.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 5., 2007, Uberaba. Anais... Londrina: Embrapa Soja, 2007. p. 109-112. (Embrapa Soja. Documentos, 292). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campos Leite, Simone Ery Grosskopf.Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Cerrados; Embrapa Soja. |
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90. | | MONTALVÃO, A. P. L.; SALA, P. I. A. L.; AMABILE, R. F.; SAYD, R. M.; CARVALHO, C. G. P. de; DIANESE, A. de C.; FAGIOLI, MARCELO. Avaliação de genótipos de girassol em ambiente de sequeiro e irrigado no Distrito Federal. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 21.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 9., 2015, Londrina. Anais... Londrina: Embrapa Soja, 2015. p. 161-164.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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91. | | GODINHO, V. de P. C.; UTUMI, M. M.; CARVALHO, C. G. P. de; CASTRO, C. de; BROGIN, R. L.; GOMES, F. F.; ALVES, J. C.; NECHEL, M. A. Avaliação de genótipos de girassol para o cerrado de Rondônia: rede nacional - final 1. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 17.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 5., 2007, Uberaba. Anais... Londrina: Embrapa Soja, 2007. p. 101-104. (Embrapa Soja. Documentos, 292). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campos Leite, Simone Ery Grosskopf.Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Soja. |
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92. | | GODINHO, V. de P. C.; UTUMI, M. M.; CARVALHO, C. G. P. de; CASTRO, C. de; BROGIN, R. L.; GOMES, F. F.; ALVES, J. C.; NECHEL, M. A. Avaliação de genótipos de girassol para o cerrado de Rondônia: rede nacional - final 1. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 17., 2007, Uberaba; SIMPÓSIO NACIONAL SOBRE CULTURA DO GIRASSOL, 5., 2007, Uberaba. Anais... Londrina: Embrapa Soja, 2007. p. 101-104. (Embrapa Soja. Documentos, 292).Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Rondônia. |
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93. | | GODINHO, V. de P. C.; UTUMI, M. M.; CARVALHO, C. G. P. de; CASTRO, C. de; BROGIN, R. L.; GOMES, F. F.; ALVES, J. C.; BAGATOLLI, K. Avaliação de genótipos de girassol para o cerrado de Rondônia: rede nacional - final 2. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 17.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 5., 2007, Uberaba. Anais... Londrina: Embrapa Soja, 2007. p. 105-108. (Embrapa Soja. Documentos, 292). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Bôas de Campos Leite, Simone Ery Grosskopf.Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Soja. |
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94. | | GODINHO, V. de P. C.; UTUMI, M. M.; CARVALHO, C. G. P. de; CASTRO, C. de; BROGIN, R. L.; GOMES, F. F.; ALVES, J. C.; BAGATOLLI, K. Avaliação de genótipos de girassol para o cerrado de Rondônia: rede nacional - final 2. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 17., 2007, Uberaba; SIMPÓSIO NACIONAL SOBRE CULTURA DO GIRASSOL, 5., 2007, Uberaba. Anais... Londrina: Embrapa Soja, 2007. p. 105-108. (Embrapa Soja. Documentos, 292).Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Rondônia. |
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95. | | LOPES, P. V. L.; MARTINS, M. C.; PANDOLFI, T. J. F.; MARQUES, C. R. G.; OLIVEIRA, A. C. B. de; CARVALHO, C. G. P. de. Avaliação de genótipos de girassol em diferentes épocas semeadura na Região Oeste da Bahia. In: CONGRESSO BRASILEIRO DE PLANTAS OLEAGINOSAS, ÓLEOS, GORDURAS E BIODIESEL, 3., 2006, Varginha, MG. Biodiesel: evolução tecnológica e qualidade: anais. Lavras: UFLA, 2006. 1 CD-ROM. pdf.: a06_184Biblioteca(s): Embrapa Soja. |
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96. | | FARIA, D. A. de; BIESDORF, E. M.; SILVA, E. B. de A.; COELHO, L. C.; CARVALHO, C. G. P. de; BORBA FILHO, A. B. Avaliação de genótipos de girassol em Mato Grosso, na safrinha de 2014. In: REUNIÃO NACIONAL DE PESQUISA DE GIRASSOL, 21.; SIMPÓSIO NACIONAL SOBRE A CULTURA DO GIRASSOL, 9., 2015, Londrina. Anais... Londrina: Embrapa Soja, 2015. p. 184-186Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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97. | | PORTO, W. S.; CARVALHO, C. G. P. de; PINTO, R. J. B.; OLIVEIRA, M. F. de; OLIVEIRA, A. C. B. de. Adaptabilidade e estabilidade de genótipos de girassol para a região subtropical do Brasil. Ciência Rural, Santa Maria, v. 39, n. 9, p. 2452-2459, dez. 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Soja. |
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98. | | CARVALHO, C. G. P. de; OZAWA, E. K. M.; AMABILE, R. F.; GODINHO, V. de P. C.; GONCALVES, S. L.; RIBEIRO, J. L.; SEIFERT, A. L. Adaptabilidade e estabilidade de genótipos de girassol resistentes a imidazolinonas em cultivos de segunda safra. Revista Brasileira de Ciências Agrárias, Recife, v. 10, n. 1, p. 1-7, 2015.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Meio-Norte; Embrapa Rondônia; Embrapa Soja. |
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99. | | CARVALHO, C. G. P. de; OZAWA, E. K. M.; AMABILE, R. F.; GODINHO, V. de P. C.; GONCALVES, S. L.; RIBEIRO, J. L.; SEIFERT, A. L. Adaptabilidade e estabilidade de genótipos de girassol resistentes a imidazolinonas em cultivos de segunda safra. Revista Brasileira de Ciências Agrárias, Recife, v. 10, n. 1, p. 1-7, 2015.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Cerrados. |
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100. | | GRUNVALD, A. K.; CARVALHO, C. G. P. de; OLIVEIRA, A. C. B. de; PIRES, J. L. F.; CARVALHO, H. W. L. de; OLIVEIRA, I. R. de. Adaptabilidade e estabilidade de híbridos de girassol convencional e alto oleico na Região Sul do Brasil. Revista de Ciências Agrárias, Belém, v. 57, n. 3, p. 217-223, jul./set. 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Soja; Embrapa Tabuleiros Costeiros. |
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