Data/hora: 21/09/2024 18:08:50

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	11/08/2021	
Data da última atualização: 	15/09/2021	
Autoria: 	ASSUNÇÃO, C. M.; MENDES, V. R. A.; BRANDAO, F. Z.; BATISTA, R. I. T. P.; SOUZA, E. D.; CARVALHO, B. C. de; QUINTAO, C. C. R.; RAPOSO, N. R. B.; CAMARGO, L. S. de A.	
Afiliação: 	CAROLINA MARINHO ASSUNÇÃO, Universidade Federal de Juiz de Fora; VIVIAN RACHEL ARAUJO MENDES, Universidade Federal de Viçosa; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense; ELIZA DINIZ SOUZA; BRUNO CAMPOS DE CARVALHO, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; NADIA REZENDE BARBOSA RAPOSO, Universidade Federal de Juiz de Fora; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.	
Título: 	Effects of resveratrol in bull semen extender on post-thaw sperm quality and capacity for fertilization and embryo development.	
Ano de publicação: 	2021	
Fonte/Imprenta: 	Animal Reproduction Science, v. 226, 106697, 2021.	
DOI: 	https://doi.org/10.1016/j.anireprosci.2021.106697	
Idioma: 	Inglês	
Conteúdo: 	Resveratrol, a potent antioxidant, can be an alternative semen extender constituent to protect spermatozoa against reactive oxygen species (ROS); however, effects on sperm quality postthawing and sperm function is not well understood. This study, therefore, was conducted to investigate effects of resveratrol supplementation to semen extender on sperm quality postthawing. Bull semen was cryopreserved using extenders not supplemented or supplemented with 0.05, 0.1, or 1 mM resveratrol. Supplementation of extender with resveratrol at 0.05 mM resulted in greater (P < 0.05) sperm progressive motility, average path velocity, straight linear velocity, linearity and straightness when compared with no or 1 mM supplementations. Furthermore, effects of 0.05 mM resveratrol supplementations on plasma membrane and acrosome integrity and sperm fertilization capacity using in vitro procedures were investigated. Supplementation of semen extender with resveratrol resulted in a greater (P < 0.05) proportion of frozen-thawed spermatozoa with an intact acrosome and plasma membrane. Results from in vitro fertilization studies indicated there were no differences (P> 0.05) when there was no supplementation or supplementation with 0.05 mM resveratrol on embryo development to the cleavage and blastocyst stages. In conclusion, addition of resveratrol to bull semen extender resulted in greater sperm quality post-thawing in a dose-dependent manner, with values for variables related to sperm quality being greater when there was resveratrol supplementation at the 0.05 mM concentration. Proportion of embryo developing to the cleavage and blastocyst stages after in vitro fertilization was not affected by resveratrol supplementation to semen extenders.	
Palavras-Chave: 	Desenvolvimento fetal; Embryo development; Fertilização in vitro.	
Thesagro: 	Bovino; Criopreservação; Esperma; Reprodução Animal.	
Thesaurus Nal: 	Antioxidants; Cryopreservation; In vitro fertilization.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25186-1) - UPC - AP - DD -  -  -  - DIS