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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/12/2008 |
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Data da última atualização: |
24/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PURCINO, A. A. C.; LIMA, T. R.; PINTO, A. C.; CARNEIRO, N. P.; SOUZA, I. R. P. de; MARRIEL, I. E.; PARENTONI, S. N.; DURAES, F.; CARVALHO, L. |
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Afiliação: |
ANTONIO ALVARO CORSETTI PURCINO, CNPMS; THALES LIMA ROCHA, CENARGEN; NEWTON PORTILHO CARNEIRO, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; SIDNEY NETTO PARENTONI, CNPMS; Frederico Durães, Embrapa Milho e Sorgo; I. Carvalho, bolsista Embrapa Recursos Genéticos e Biotecnologia. |
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Título: |
Glutamine synthetase response to nitrate in maize genotypes of contrasting nitrogen use efficiency. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Maydica, Bergamo, v. 53, p. 101- 109, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
Although nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Altogether, these results suggest that breeding maize inbred !ines for better NUE, indirectly selected plants with an increased GSl content in rclation to GS2, without a significant effect on total GS biosynthetie activity. NUE in the singlc cross hybrids werc less influenccd by the GSl:GS2 ratio, but one possiblc explanation for this discrepancy may have becn ascribcd to the less imense sc!ection pressure for increased NUE undergone by the hybrids. MenosAlthough nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Alt... Mostrar Tudo |
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Palavras-Chave: |
Glutamine synthetase; Nitrogcn use effi-cicncy; Nitrogen use efficiency; Zea mays L. |
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Thesagro: |
Milho; Zea Mays. |
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Categoria do assunto: |
--
S Ciências Biológicas |
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Marc: |
LEADER 02778naa a2200289 a 4500
001 1491623
005 2018-05-24
008 2008 bl uuuu u00u1 u #d
100 1 $aPURCINO, A. A. C.
245 $aGlutamine synthetase response to nitrate in maize genotypes of contrasting nitrogen use efficiency.$h[electronic resource]
260 $c2008
520 $aAlthough nitrogen use efficiency (NUE) is a complex trait. recent evidences have indicated that glutamine synthetase (GS, EC 6.3.1.2) is one of its major components. ln maize. GS is encoded by a multigene family and its gene products can be resolved into cytosolic (GSl) and chloroplastic (GS2) isoforms. Thercfore, the objective of this study was to determine possible systematie patterns of GS activity or isoenzyme expression response to nitrate nutrition among five maize (Zea mays 1.) SS inbred lines and five single cross hybrids, previously selected for contrasting NUE. Leaf GS biosynthetie activity significantly responded to nitrate nutrition at the 4-leaf seedling and at the 2-day after silking stages. but breeding for increased NUE did not lead to the development of genotypes with increased GS activity. After native polyacrylamide gcl electrophoreses (native-PAGE) and staining for transferase activity, GS produced distinct "up-per" and "lower" activity bands. ln leaves, these bands did not show a clear nitrate effect pattem, but in roots, the "upper" bands seemed to be more active in high nitrate plants, independently of the NUE of the genotypes tested. Protein gel analysis (SDS-PAGE), however. indicat-ed that breeding for increased NUE, under both low and high nitrate nutrition, led to the devclopment of inbred lines with more GSl than GS2 Cincreased GSl:GS2 ratio). Except for CMS 28-17.2 under low nitrate, this trend was not observed among the low NUE inbred lines. Altogether, these results suggest that breeding maize inbred !ines for better NUE, indirectly selected plants with an increased GSl content in rclation to GS2, without a significant effect on total GS biosynthetie activity. NUE in the singlc cross hybrids werc less influenccd by the GSl:GS2 ratio, but one possiblc explanation for this discrepancy may have becn ascribcd to the less imense sc!ection pressure for increased NUE undergone by the hybrids.
650 $aMilho
650 $aZea Mays
653 $aGlutamine synthetase
653 $aNitrogcn use effi-cicncy
653 $aNitrogen use efficiency
653 $aZea mays L
700 1 $aLIMA, T. R.
700 1 $aPINTO, A. C.
700 1 $aCARNEIRO, N. P.
700 1 $aSOUZA, I. R. P. de
700 1 $aMARRIEL, I. E.
700 1 $aPARENTONI, S. N.
700 1 $aDURAES, F.
700 1 $aCARVALHO, L.
773 $tMaydica, Bergamo$gv. 53, p. 101- 109, 2008.
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Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
02/03/2022 |
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Data da última atualização: |
10/10/2022 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
FREITAS-SILVA, O.; COELHO, C. C. DE S.; TROMBETE, F. M.; CONCEIÇÃO, R. R. P. DA; RIBEIRO-SANTOS, R. |
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Afiliação: |
OTNIEL FREITAS SILVA, CTAA; CAROLINE CORRÊA DE SOUZA COELHO, UNIRIO; FELIPE MACHADO TROMBETE, UFSJ; RENATA REGINA PEREIRA DA CONCEIÇÃO, UFMG; REGIANE RIBEIRO-SANTOS, IFPE. |
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Título: |
Chemical Degradation of Afatoxins. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: Khalid Rehman HAKEEM, K. R.; OLIVEIRA, C. A. F. DE; ISMAIL, A. (ed.). Aflatoxins in Food: A Recent Perspective. Springer, cap. 11, p. 233-258, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
The removal of toxigenic fungi species from the genus Aspergillus and their highly toxic aflatoxins from contaminated foodstuffs is a difficult task, in view of their high resistance to heat treatments and solubility only in intermediate polar solvents. In this regard, chemical methods have been proposed for decontamination of aflatoxins (AFs) in foodstuffs, including ozonation, application of organic acids, hydrogen peroxide, and plant extracts, among other compounds. However, the production and use of chemical compounds are subjected to strong legislative pressure. This chapter presents the state of the art on the control of mycotoxins regarding the application of chemicals for the reduction of fungi and degradation of AFs, as well as corresponding regulatory and food safety issues. One of the most relevant chemical methods is the ozonated water which, in addition to being effective in AF detoxification, also eliminates pathogenic agents and microorganisms, contributing to food safety. However, the choice of the chemical product, concentration, time, and way of application generally has a large impact on the sensory attributes and nutritional values of the treated foods. In addition, residues from chemical used for aflatoxin decontamination can cause direct damage to human and animal health or induce negative effects through interaction with other nutrients. Further studies are necessary to better understand the mechanisms of detoxification by chemical compounds aiming at their application on an industrial scale, MenosThe removal of toxigenic fungi species from the genus Aspergillus and their highly toxic aflatoxins from contaminated foodstuffs is a difficult task, in view of their high resistance to heat treatments and solubility only in intermediate polar solvents. In this regard, chemical methods have been proposed for decontamination of aflatoxins (AFs) in foodstuffs, including ozonation, application of organic acids, hydrogen peroxide, and plant extracts, among other compounds. However, the production and use of chemical compounds are subjected to strong legislative pressure. This chapter presents the state of the art on the control of mycotoxins regarding the application of chemicals for the reduction of fungi and degradation of AFs, as well as corresponding regulatory and food safety issues. One of the most relevant chemical methods is the ozonated water which, in addition to being effective in AF detoxification, also eliminates pathogenic agents and microorganisms, contributing to food safety. However, the choice of the chemical product, concentration, time, and way of application generally has a large impact on the sensory attributes and nutritional values of the treated foods. In addition, residues from chemical used for aflatoxin decontamination can cause direct damage to human and animal health or induce negative effects through interaction with other nutrients. Further studies are necessary to better understand the mechanisms of detoxification by chemical compounds aiming at ... Mostrar Tudo |
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Palavras-Chave: |
Ammonization; Chemical detoxification; Organic acids. |
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Thesaurus NAL: |
Aflatoxins; Food technology; Ozone; Plant extracts. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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Marc: |
LEADER 02332naa a2200253 a 4500
001 2140471
005 2022-10-10
008 2021 bl uuuu u00u1 u #d
100 1 $aFREITAS-SILVA, O.
245 $aChemical Degradation of Afatoxins.$h[electronic resource]
260 $c2021
520 $aThe removal of toxigenic fungi species from the genus Aspergillus and their highly toxic aflatoxins from contaminated foodstuffs is a difficult task, in view of their high resistance to heat treatments and solubility only in intermediate polar solvents. In this regard, chemical methods have been proposed for decontamination of aflatoxins (AFs) in foodstuffs, including ozonation, application of organic acids, hydrogen peroxide, and plant extracts, among other compounds. However, the production and use of chemical compounds are subjected to strong legislative pressure. This chapter presents the state of the art on the control of mycotoxins regarding the application of chemicals for the reduction of fungi and degradation of AFs, as well as corresponding regulatory and food safety issues. One of the most relevant chemical methods is the ozonated water which, in addition to being effective in AF detoxification, also eliminates pathogenic agents and microorganisms, contributing to food safety. However, the choice of the chemical product, concentration, time, and way of application generally has a large impact on the sensory attributes and nutritional values of the treated foods. In addition, residues from chemical used for aflatoxin decontamination can cause direct damage to human and animal health or induce negative effects through interaction with other nutrients. Further studies are necessary to better understand the mechanisms of detoxification by chemical compounds aiming at their application on an industrial scale,
650 $aAflatoxins
650 $aFood technology
650 $aOzone
650 $aPlant extracts
653 $aAmmonization
653 $aChemical detoxification
653 $aOrganic acids
700 1 $aCOELHO, C. C. DE S.
700 1 $aTROMBETE, F. M.
700 1 $aCONCEIÇÃO, R. R. P. DA
700 1 $aRIBEIRO-SANTOS, R.
773 $tIn: Khalid Rehman HAKEEM, K. R.; OLIVEIRA, C. A. F. DE; ISMAIL, A. (ed.). Aflatoxins in Food: A Recent Perspective. Springer, cap. 11, p. 233-258, 2021.
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