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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
28/09/2020 |
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Data da última atualização: |
07/12/2020 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
CARNEIRO, M. T.; PEREZ, D. V.; FEITOSA, R. C.; WASSERMAN, J. C. |
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Afiliação: |
MARCOS TAVARES CARNEIRO, UFF/FIOCRUZ; DANIEL VIDAL PEREZ, CNPS; RENATO CASTIGLIA FEITOSA, FIOCRUZ; JULIO CESAR WASSERMAN, UFF. |
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Título: |
Separation of Escherichia coli from natural samples for identification of sources and microcosm inoculation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal Of Microbiology, v. 51, p. 2015-2020, 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00374-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Obtaining uncultured Escherichia coli from natural waters is an important step in the study of microbes in the environment, which are critical for bacterial decay and microbial source tracking. The quality of the samples used can influence the assays, because high contaminant concentrations, differing cell ages, and physiologic states can impair results. The proposed separation is based on a three-step filtration method applied to replicates of seven samples from a sewage plant affluent, collected in different periods. Aliquots of the leachate were inoculated into microcosms, aiming to observe the cultivability of the cells. The assay resulted in colimetry values ranging between 104 and 105 cells. In the leachate, averages of 1.05% of total coliforms and 1.10% of Escherichia coli were recovered from original samples. Although enduring unfavorable temperatures, salinities, and nutritional conditions, the inoculated microcosm populations grew approximately 310 times after 24 h. The final leachate contained cultivable cells in appropriate physiological states and quantities for inoculum in microcosm sets. The bacteria obtained from the leachate were also appropriate for surveys of microbial source tracking, because, in the developed procedure, organisms were separated from contaminants, while cell concentrations were sufficient for inocula. |
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Thesagro: |
Águas Residuais; Bactéria; Escherichia Coli; Rastreamento. |
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Thesaurus Nal: |
Strains; Wastewater. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218815/1/Separation-of-Escherichia-coli-from-natural-samples-2020.pdf
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Marc: |
LEADER 02106naa a2200241 a 4500
001 2125143
005 2020-12-07
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00374-2$2DOI
100 1 $aCARNEIRO, M. T.
245 $aSeparation of Escherichia coli from natural samples for identification of sources and microcosm inoculation.$h[electronic resource]
260 $c2020
520 $aObtaining uncultured Escherichia coli from natural waters is an important step in the study of microbes in the environment, which are critical for bacterial decay and microbial source tracking. The quality of the samples used can influence the assays, because high contaminant concentrations, differing cell ages, and physiologic states can impair results. The proposed separation is based on a three-step filtration method applied to replicates of seven samples from a sewage plant affluent, collected in different periods. Aliquots of the leachate were inoculated into microcosms, aiming to observe the cultivability of the cells. The assay resulted in colimetry values ranging between 104 and 105 cells. In the leachate, averages of 1.05% of total coliforms and 1.10% of Escherichia coli were recovered from original samples. Although enduring unfavorable temperatures, salinities, and nutritional conditions, the inoculated microcosm populations grew approximately 310 times after 24 h. The final leachate contained cultivable cells in appropriate physiological states and quantities for inoculum in microcosm sets. The bacteria obtained from the leachate were also appropriate for surveys of microbial source tracking, because, in the developed procedure, organisms were separated from contaminants, while cell concentrations were sufficient for inocula.
650 $aStrains
650 $aWastewater
650 $aÁguas Residuais
650 $aBactéria
650 $aEscherichia Coli
650 $aRastreamento
700 1 $aPEREZ, D. V.
700 1 $aFEITOSA, R. C.
700 1 $aWASSERMAN, J. C.
773 $tBrazilian Journal Of Microbiology$gv. 51, p. 2015-2020, 2020.
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Registro original: |
Embrapa Solos (CNPS) |
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Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
14/11/1997 |
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Data da última atualização: |
03/04/2023 |
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Autoria: |
FAZOLIN, M.; ARGOLO, V. M.; ESTRELA, J. L. V. |
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Afiliação: |
MURILO FAZOLIN, CPAF-Acre; VALDIRENE MAIA ARGOLO, BOLSISTA UFAC; JOELMA LIMA VIDAL ESTRELA, Bolsista CNPq/RHAE. |
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Título: |
Proposta para maximizar a utilização dos recursos disponíveis na criação do besouro africano (Onthophagus gazella, Fab.). |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Rio Branco, AC: Embrapa CPAF-AC, 1997. |
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Páginas: |
16 p. |
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Série: |
(Embrapa CPAF-AC. Boletim de pesquisa, 16). |
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ISSN: |
0101-5516 |
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Idioma: |
Português |
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Conteúdo: |
Este estudo tem a finalidade de averiguar as alterações em cruzamentos de O. gazella em laboratório e maximizar a utilização dos recursos físicos e de mão-de-obra na produção massal desse inseto. |
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Palavras-Chave: |
Agentes de control biológico; Animal pests; Besouro africano; Brown dung beetle; Control biológico; Horn fly; Mejoramiento genético de insectos; Plagas de animales; Plagas de insectos; Plagas y parásitos de animales; Variación genética. |
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Thesagro: |
Besouro; Bovino; Controle biológico; Criação massal; Haematobia irritans; Inseto para controle biológico; Melhoramento genético animal; Mosca dos chifres; Praga de animal; Variação genética. |
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Thesaurus NAL: |
Animal parasites and pests; Biological control; Biological control agents; Genetic variation; Insect breeding; Insect pests; Onthophagus gazella. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/CPAF-AC/1259/1/bp16.pdf
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Marc: |
LEADER 01771nam a2200505 a 4500
001 1492547
005 2023-04-03
008 1997 bl uuuu u0uu1 u #d
022 $a0101-5516
100 1 $aFAZOLIN, M.
245 $aProposta para maximizar a utilização dos recursos disponíveis na criação do besouro africano (Onthophagus gazella, Fab.).$h[electronic resource]
260 $aRio Branco, AC: Embrapa CPAF-AC$c1997
300 $a16 p.
490 $a(Embrapa CPAF-AC. Boletim de pesquisa, 16).
520 $aEste estudo tem a finalidade de averiguar as alterações em cruzamentos de O. gazella em laboratório e maximizar a utilização dos recursos físicos e de mão-de-obra na produção massal desse inseto.
650 $aAnimal parasites and pests
650 $aBiological control
650 $aBiological control agents
650 $aGenetic variation
650 $aInsect breeding
650 $aInsect pests
650 $aOnthophagus gazella
650 $aBesouro
650 $aBovino
650 $aControle biológico
650 $aCriação massal
650 $aHaematobia irritans
650 $aInseto para controle biológico
650 $aMelhoramento genético animal
650 $aMosca dos chifres
650 $aPraga de animal
650 $aVariação genética
653 $aAgentes de control biológico
653 $aAnimal pests
653 $aBesouro africano
653 $aBrown dung beetle
653 $aControl biológico
653 $aHorn fly
653 $aMejoramiento genético de insectos
653 $aPlagas de animales
653 $aPlagas de insectos
653 $aPlagas y parásitos de animales
653 $aVariación genética
700 1 $aARGOLO, V. M.
700 1 $aESTRELA, J. L. V.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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