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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/04/2019 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SABINO, Y. N. V.; FOCHAT, R. C.; LIMA, J. C. F.; RIBEIRO, M. T.; ARCURI, P. B.; CARNEIRO, J. da C.; MACHADO, M. A.; REIS, D. R. de L.; MACHADO, A. B. F.; HÚNGARO, H. M.; RIBEIRO, J. B.; PAIVA, A. D. |
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Afiliação: |
Yasmin Neves Vieira Sabino, UFJF; Romário Costa Fochat, UFJF; JUNIOR CESAR FERNANDES LIMA, CNPGL; MARLICE TEIXEIRA RIBEIRO, CNPGL; PEDRO BRAGA ARCURI, CNPGL; JAILTON DA COSTA CARNEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; Alessandra Barbosa Ferreira Machado, UFTM, Uberaba; Humberto Moreira Húngaro, UFJF; JOAO BATISTA RIBEIRO, CNPGL; Aline Dias Paiva, UFTM, Uberaba. |
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Título: |
Antibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Annals of Microbiology, v. 69, p. 131-138, 2019. |
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DOI: |
10.1007/s13213-018-1407-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem. |
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Palavras-Chave: |
Antibacterial activity; Bacteriocin; LanC; Lantibiotic; Streptococcus sp. |
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Thesagro: |
Rúmen. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02399naa a2200337 a 4500
001 2108297
005 2023-01-24
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s13213-018-1407-2$2DOI
100 1 $aSABINO, Y. N. V.
245 $aAntibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid.$h[electronic resource]
260 $c2019
520 $aAbstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem.
650 $aRúmen
653 $aAntibacterial activity
653 $aBacteriocin
653 $aLanC
653 $aLantibiotic
653 $aStreptococcus sp
700 1 $aFOCHAT, R. C.
700 1 $aLIMA, J. C. F.
700 1 $aRIBEIRO, M. T.
700 1 $aARCURI, P. B.
700 1 $aCARNEIRO, J. da C.
700 1 $aMACHADO, M. A.
700 1 $aREIS, D. R. de L.
700 1 $aMACHADO, A. B. F.
700 1 $aHÚNGARO, H. M.
700 1 $aRIBEIRO, J. B.
700 1 $aPAIVA, A. D.
773 $tAnnals of Microbiology$gv. 69, p. 131-138, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
15/01/2020 |
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Data da última atualização: |
20/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MACÊDO, E. C. F. de; ZONTA, J. H.; MELO, Y. L.; MELO, A. S. de; SILVA, D. C. da; ANDRADE, W. L. de. |
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Afiliação: |
Érika C. F. de Macêdo, Universidade Estadual da Paraíba - UEPB; JOAO HENRIQUE ZONTA, CNPA; Yuri L. Melo, Universidade Estadual da Paraíba - UEPB; Alberto S. de Melo, Universidade Estadual da Paraíba - UEPB; Duval C. da Silva, Universidade Estadual da Paraíba - UEPB; Wellerson L. de Andrade, Universidade Estadual da Paraíba - UEPB. |
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Título: |
Changes in osmoregulatory metabolism of cotton genotypes during water deficit and recovery period. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Revista Brasileira de Engenharia Agrícola e Ambiental, v. 23, n. 8, p. 607-613, 2019. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Mudanças no metabolismo osmorregulador de genótipos de algodoeiro durante déficit hídrico e período de recuperação. |
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Conteúdo: |
The aim of this study was to evaluate the changes in osmoregulatory metabolism of cotton genotypes subjected to water deficit and recovery period. In a greenhouse, six cotton genotypes and two water managements were combined in a 6 x 2 factorial scheme, in a completely randomized design, evaluated after 14 and 22 days under water stress and recovery conditions, respectively. The water status, growth indicators and compatible solutes in the leaves were evaluated. The genotypes BRS Seridó, BRS Aroeira and BRS 7MH withstand better the stress condition because they had lower variations in their water potentials due to the osmotic adjustment and maintained their growth under water deficit conditions. The BRS 286 is the most sensitive genotype to the water deficit condition, since it showed greater variations in its water potential, which ultimately stopped its growth. Water replacement during the recovery period allowed growth resumption in most of the genotypes, mainly CNPA 5M and BRS 286. After the recovery period, variations in the concentrations of osmoregulators indicate the plasticity of cotton in regulating the concentrations of osmoregulators under favorable and unfavorable water conditions. |
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Palavras-Chave: |
Ajustamento osmótico; Compatible osmolytes; Osmólito compatível; Osmotic adjustment. |
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Thesagro: |
Gossypium Hirsutum; Seca. |
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Thesaurus NAL: |
Drought. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208800/1/Changes-in-osmoregulatory-metabolism.pdf
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Marc: |
LEADER 02196naa a2200277 a 4500
001 2118783
005 2020-01-20
008 2019 bl uuuu u00u1 u #d
100 1 $aMACÊDO, E. C. F. de
245 $aChanges in osmoregulatory metabolism of cotton genotypes during water deficit and recovery period.$h[electronic resource]
260 $c2019
500 $aTítulo em português: Mudanças no metabolismo osmorregulador de genótipos de algodoeiro durante déficit hídrico e período de recuperação.
520 $aThe aim of this study was to evaluate the changes in osmoregulatory metabolism of cotton genotypes subjected to water deficit and recovery period. In a greenhouse, six cotton genotypes and two water managements were combined in a 6 x 2 factorial scheme, in a completely randomized design, evaluated after 14 and 22 days under water stress and recovery conditions, respectively. The water status, growth indicators and compatible solutes in the leaves were evaluated. The genotypes BRS Seridó, BRS Aroeira and BRS 7MH withstand better the stress condition because they had lower variations in their water potentials due to the osmotic adjustment and maintained their growth under water deficit conditions. The BRS 286 is the most sensitive genotype to the water deficit condition, since it showed greater variations in its water potential, which ultimately stopped its growth. Water replacement during the recovery period allowed growth resumption in most of the genotypes, mainly CNPA 5M and BRS 286. After the recovery period, variations in the concentrations of osmoregulators indicate the plasticity of cotton in regulating the concentrations of osmoregulators under favorable and unfavorable water conditions.
650 $aDrought
650 $aGossypium Hirsutum
650 $aSeca
653 $aAjustamento osmótico
653 $aCompatible osmolytes
653 $aOsmólito compatível
653 $aOsmotic adjustment
700 1 $aZONTA, J. H.
700 1 $aMELO, Y. L.
700 1 $aMELO, A. S. de
700 1 $aSILVA, D. C. da
700 1 $aANDRADE, W. L. de
773 $tRevista Brasileira de Engenharia Agrícola e Ambiental$gv. 23, n. 8, p. 607-613, 2019.
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Embrapa Algodão (CNPA) |
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