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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia; Embrapa Suínos e Aves. |
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Data corrente: |
27/03/1997 |
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Data da última atualização: |
09/07/2007 |
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Autoria: |
CARNEIRO, G. G. |
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Título: |
Eficiencia reprodutiva e produtividade em suinos. |
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Ano de publicação: |
1958 |
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Fonte/Imprenta: |
Belo Horizonte: Universidade Rural do Estado de Minas Gerais, 1958. |
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Páginas: |
99p. |
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Idioma: |
Português |
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Notas: |
Tese Livre-Docencia |
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Conteúdo: |
Foram estudados os dados de 160 porcas e 445 leitegadas racialmente puras, de 1949 a 1956 inclusive, com o objeto principal de identificar e descrever os fatores relativos a eficiencia reprodutiva e a produtividade de porcas, mantidas na condicoes de alimentacao e manejo geral adotadas no Instituto de Zootecnia de Minas Gerais. Foi tambem estimado o grau de "repetibilidade" (repeatability) da produtividade das porcas, medido pelo numero de leitoes na leitegada e peso total da leitegada em cada uma das tres seguintes idades dessa leitegada: nascimento, terceira e oitava semanas. |
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Palavras-Chave: |
Desempenho reprodutivo; Fertility; Porcas; Productivity; Sow. |
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Thesagro: |
Fertilidade; Melhoramento Animal; Produtividade; Reprodução; Suíno. |
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Thesaurus Nal: |
reproductive performance; swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01307nam a2200277 a 4500
001 1431997
005 2007-07-09
008 1958 bl uuuu m 00u1 u #d
100 1 $aCARNEIRO, G. G.
245 $aEficiencia reprodutiva e produtividade em suinos.
260 $aBelo Horizonte: Universidade Rural do Estado de Minas Gerais$c1958
300 $a99p.
500 $aTese Livre-Docencia
520 $aForam estudados os dados de 160 porcas e 445 leitegadas racialmente puras, de 1949 a 1956 inclusive, com o objeto principal de identificar e descrever os fatores relativos a eficiencia reprodutiva e a produtividade de porcas, mantidas na condicoes de alimentacao e manejo geral adotadas no Instituto de Zootecnia de Minas Gerais. Foi tambem estimado o grau de "repetibilidade" (repeatability) da produtividade das porcas, medido pelo numero de leitoes na leitegada e peso total da leitegada em cada uma das tres seguintes idades dessa leitegada: nascimento, terceira e oitava semanas.
650 $areproductive performance
650 $aswine
650 $aFertilidade
650 $aMelhoramento Animal
650 $aProdutividade
650 $aReprodução
650 $aSuíno
653 $aDesempenho reprodutivo
653 $aFertility
653 $aPorcas
653 $aProductivity
653 $aSow
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
24/03/2008 |
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Data da última atualização: |
22/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M. |
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Afiliação: |
Naiara Z. Saraiva, UNESP/Jaboticabal; Felipe Perecin USP/Pirassununga Simone Crisitina Méo CPPSE; Christina Ramires Ferreira, Unicamp; Tatiane Almeida D. Tetzner, UNESP/Jaboticabal; J. M. Oliveira, UNESP/Jaboticabal; Joaquim Mansano Garcia, UNESP/Jaboticabal. |
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Título: |
Effects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 20, n. 1, p. 107-108, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
The developmental competence of enucleated oocytes is a key factor that determines the overall success of animal cloning. Enucleation is an invasive procedure in traditional nuclear transfer (NT). The objective of this work was to evaluate the effects of demecolcine, a microtubule-depolymerizing agent, on metaphase II (MII) bovine oocytes and to verify the capacity of embryonic development after NT using chemically assisted enucleation. In the first experiment, oocytes after 21 h of IVM were exposed for 2 h to several concentrations of demecolcine: 0 (control), 0.025, 0.05, 0.2, and 0.4 µg mL?1, and evaluated in relation to membrane protrusion formation. After the best concentration of demecolcine was determined, the nuclear and microtubular dynamics of the treated oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 5, 537?545) in a second experiment. The results were analyzed by Duncan and Tukey tests in Experiments I and II, respectively, and a level of 5% significance was used. In Experiment III, embryonic development following NT was determined using adult donor cells, derived from a skin biopsy. After 19 h of IVM, oocytes were exposed to demecolcine (0.05 µg mL?1) for 2 h, and enucleation was performed in oocytes that presented membrane protrusions. Samples of cytoplasts were stained with Hoechst 33342 for 10 min (10 µg mL?1) for evaluation of enucleation effectiveness. The remaining oocytes were reconstituted by NT, fused (two pulses of 2.0 kV cm?1 for 20 µs each, in 0.28 m mannitol solution), chemically activated (5 mm ionomycin for 5 min and 2 mm 6-DMAP for 4 h), and cultured in SOF medium with 2.5% FCS and 0.5% BSA. The 0.05 µg mL?1 concentration resulted in greater protrusion rates (55.1%; 211/388) in comparison to 0, 0.025, 0.2, and 0.4 µg mL?1 concentrations (0, 42.6, 45.1, and 39.3%, respectively). In Experiment II, at the beginning of treatment, the majority of the oocytes were in MII (40.7%) or anaphase I/telophase I (AI/TI; 22.4%). Effects of demecolcine occurred within only 0.5 h of treatment, with a significant increase in oocytes with complete depletion of microtubules (21.8%; initial average: 1.9%) and a reduction in the proportion at MII (13.5%) and AI/TI (8.2%). New polymerization of microtubules was observed when treated oocytes were then cultured in drug-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). In Experiment III, we verified the effectiveness of the chemically assisted enucleation technique (90.6%; 77/85). We evaluated 515 oocytes, of which 219 (42.5%) had protrusions and were enucleated. After losses in the NT procedure and fusion, 58 reconstructed 1-cells were cultured resulting in cleavage and blastocyst rates of 84.5% (49/58) and 27.6% (16/58), respectively, with great variation in blastocyst production between the three replicates (12.5% to 47%). In conclusion, demecolcine can be used at lower concentrations than those used routinely, and the chemically assisted enucleation method has proven highly efficient and does not appear to inhibit embryonic development in the bovine MenosThe developmental competence of enucleated oocytes is a key factor that determines the overall success of animal cloning. Enucleation is an invasive procedure in traditional nuclear transfer (NT). The objective of this work was to evaluate the effects of demecolcine, a microtubule-depolymerizing agent, on metaphase II (MII) bovine oocytes and to verify the capacity of embryonic development after NT using chemically assisted enucleation. In the first experiment, oocytes after 21 h of IVM were exposed for 2 h to several concentrations of demecolcine: 0 (control), 0.025, 0.05, 0.2, and 0.4 µg mL?1, and evaluated in relation to membrane protrusion formation. After the best concentration of demecolcine was determined, the nuclear and microtubular dynamics of the treated oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 5, 537?545) in a second experiment. The results were analyzed by Duncan and Tukey tests in Experiments I and II, respectively, and a level of 5% significance was used. In Experiment III, embryonic development following NT was determined using adult donor cells, derived from a skin biopsy. After 19 h of IVM, oocytes were exposed to demecolcine (0.05 µg mL?1) for 2 h, and enucleation was performed in oocytes that presented membrane protrusions. Samples of cytoplasts were stained with Hoechst 33342 for 10 min (10 µg mL?1) for evaluation of enucleation effectiveness. The remaining oocytes were reconstituted ... Mostrar Tudo |
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Thesagro: |
BOVINO. |
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Thesaurus NAL: |
oocytes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42090/1/PROCI-2008.00005.pdf
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Marc: |
LEADER 03789nam a2200205 a 4500
001 1048309
005 2023-06-22
008 2008 bl uuuu u00u1 u #d
100 1 $aSARAIVA, N. Z.
245 $aEffects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes.$h[electronic resource]
260 $aReproduction, Fertility and Development, v. 20, n. 1, p. 107-108$c2008
520 $aThe developmental competence of enucleated oocytes is a key factor that determines the overall success of animal cloning. Enucleation is an invasive procedure in traditional nuclear transfer (NT). The objective of this work was to evaluate the effects of demecolcine, a microtubule-depolymerizing agent, on metaphase II (MII) bovine oocytes and to verify the capacity of embryonic development after NT using chemically assisted enucleation. In the first experiment, oocytes after 21 h of IVM were exposed for 2 h to several concentrations of demecolcine: 0 (control), 0.025, 0.05, 0.2, and 0.4 µg mL?1, and evaluated in relation to membrane protrusion formation. After the best concentration of demecolcine was determined, the nuclear and microtubular dynamics of the treated oocytes were evaluated by immunofluorescence microscopy of tubulin and chromatin (Liu et al. 1998 Biol. Reprod. 5, 537?545) in a second experiment. The results were analyzed by Duncan and Tukey tests in Experiments I and II, respectively, and a level of 5% significance was used. In Experiment III, embryonic development following NT was determined using adult donor cells, derived from a skin biopsy. After 19 h of IVM, oocytes were exposed to demecolcine (0.05 µg mL?1) for 2 h, and enucleation was performed in oocytes that presented membrane protrusions. Samples of cytoplasts were stained with Hoechst 33342 for 10 min (10 µg mL?1) for evaluation of enucleation effectiveness. The remaining oocytes were reconstituted by NT, fused (two pulses of 2.0 kV cm?1 for 20 µs each, in 0.28 m mannitol solution), chemically activated (5 mm ionomycin for 5 min and 2 mm 6-DMAP for 4 h), and cultured in SOF medium with 2.5% FCS and 0.5% BSA. The 0.05 µg mL?1 concentration resulted in greater protrusion rates (55.1%; 211/388) in comparison to 0, 0.025, 0.2, and 0.4 µg mL?1 concentrations (0, 42.6, 45.1, and 39.3%, respectively). In Experiment II, at the beginning of treatment, the majority of the oocytes were in MII (40.7%) or anaphase I/telophase I (AI/TI; 22.4%). Effects of demecolcine occurred within only 0.5 h of treatment, with a significant increase in oocytes with complete depletion of microtubules (21.8%; initial average: 1.9%) and a reduction in the proportion at MII (13.5%) and AI/TI (8.2%). New polymerization of microtubules was observed when treated oocytes were then cultured in drug-free medium for 6 h (42.4% oocytes with two evident sets of microtubules). In Experiment III, we verified the effectiveness of the chemically assisted enucleation technique (90.6%; 77/85). We evaluated 515 oocytes, of which 219 (42.5%) had protrusions and were enucleated. After losses in the NT procedure and fusion, 58 reconstructed 1-cells were cultured resulting in cleavage and blastocyst rates of 84.5% (49/58) and 27.6% (16/58), respectively, with great variation in blastocyst production between the three replicates (12.5% to 47%). In conclusion, demecolcine can be used at lower concentrations than those used routinely, and the chemically assisted enucleation method has proven highly efficient and does not appear to inhibit embryonic development in the bovine
650 $aoocytes
650 $aBOVINO
700 1 $aPERECIN, F.
700 1 $aMÉO, S. C.
700 1 $aFERREIRA, C. R.
700 1 $aTETZNER, T. A. D.
700 1 $aOLIVEIRA, J. C.
700 1 $aGARCIA, J. M.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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