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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
11/05/2020 |
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Data da última atualização: |
15/07/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARMO, C. D. do; SOUSA, M. B. e; SILVA, P. P. dos S.; OLIVEIRA, G. A. F.; CEBALLOS, H.; OLIVEIRA, E. J. de. |
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Afiliação: |
CÁTIA DIAS DO CARMO; MASSAINE BANDEIRA E SOUSA; PRISCILA PATRÍCIA DOS SANTOS SILVA; GILMARA ALVARENGA FACHARDO OLIVEIRA; HERNÁN CEBALLOS; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Identification and validation of mutation points associated with waxy phenotype in cassava. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 20, p.164, 2020. |
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ISSN: |
1471-2229 |
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Idioma: |
Inglês |
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Conteúdo: |
BACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR (KASP) markers that allowed discrimination between WX and wx alleles. The results demonstrated the non-existence of heterozygous individuals of the MeWxEx6-del-C deletion in the analyzed germplasm. Therefore, the deletion MeWxEx6-del-C should not be used for assisted selection in genetic backgrounds different from the original source of waxy starch. Also, the alternative SNPs identified in this study were not associated with the waxy phenotype when compared to a panel of accessions with high genetic diversity. CONCLUSION: Although the GBSSI gene can exhibit several mutations in cassava, only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype in the original AM206-5 source. MenosBACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
Cassava. |
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Categoria do assunto: |
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Marc: |
LEADER 02827naa a2200217 a 4500
001 2122191
005 2020-07-15
008 2020 bl uuuu u00u1 u #d
022 $a1471-2229
100 1 $aCARMO, C. D. do
245 $aIdentification and validation of mutation points associated with waxy phenotype in cassava.$h[electronic resource]
260 $c2020
520 $aBACKGROUND: The granule-bound starch synthase I (GBSSI) enzyme is responsible for the synthesis of amylose, and therefore, its absence results in individuals with a waxy starch phenotype in various amylaceous crops. The validation of mutation points previously associated with the waxy starch phenotype in cassava, as well as the identification of alternative mutant alleles in the GBSSI gene, can allow the development of molecular-assisted selection to introgress the waxy starch mutation into cassava breeding populations. RESULTS: A waxy cassava allele has been identified previously, associated with several SNPs. A particular SNP (intron 11) was used to develop SNAP markers for screening heterozygote types in cassava germplasm. Although the molecular segregation corresponds to the expected segregation at 3:1 ratio (dominant gene for the presence of amylose), the homozygotes containing the SNP associated with the waxy mutation did not show waxy phenotypes. To identify more markers, we sequenced the GBSS gene from 89 genotypes, including some that were segregated from a cross with a line carrying the known waxy allele. As a result, 17 mutations in the GBSSI gene were identified, in which only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype. The evaluation of mutation points by discriminant analysis of principal component analysis (DAPC) also did not completely discriminate the waxy individuals. Therefore, we developed Kompetitive Allele Specific PCR (KASP) markers that allowed discrimination between WX and wx alleles. The results demonstrated the non-existence of heterozygous individuals of the MeWxEx6-del-C deletion in the analyzed germplasm. Therefore, the deletion MeWxEx6-del-C should not be used for assisted selection in genetic backgrounds different from the original source of waxy starch. Also, the alternative SNPs identified in this study were not associated with the waxy phenotype when compared to a panel of accessions with high genetic diversity. CONCLUSION: Although the GBSSI gene can exhibit several mutations in cassava, only the deletion in exon 6 (MeWxEx6-del-C) was correlated with the waxy phenotype in the original AM206-5 source.
650 $aCassava
650 $aMandioca
700 1 $aSOUSA, M. B. e
700 1 $aSILVA, P. P. dos S.
700 1 $aOLIVEIRA, G. A. F.
700 1 $aCEBALLOS, H.
700 1 $aOLIVEIRA, E. J. de
773 $tBMC Plant Biology$gv. 20, p.164, 2020.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
20/09/2006 |
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Data da última atualização: |
06/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, W. J. da; SANS, L. M. A.; MAGALHAES, P. C.; DURAES, F. O. M. |
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Afiliação: |
Embrapa Milho e Sorgo; PAULO CESAR MAGALHAES, CNPMS; FREDERICO OZANAN MACHADO DURAES, SPM GGE. |
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Título: |
Exigências climáticas do milho em sistema plantio direto. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Informe Agropecuário, Belo Horizonte, v. 27, n. 233, p. 14-25, jul./ago. 2006. |
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Idioma: |
Português |
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Conteúdo: |
A produtividade do milho depende do número de grãos potencialmente capazes de se desenvolver, e o enchimento desses grãos, dos fatores ambientais. A intensidade com que a cultura do milho expressa seu potencial genético é determinada por sua interação com o regime de radiação solar, com a temperatura, com o déficit de pressão de vapor, com a velocidade do vento e com as características físico-hídricas do solo. Aparentemente, não existe limite máximo de temperatura para a produção de milho, no entanto, a produtividade tende a diminuir com o aumento dela. As exigências térmicas do milho, da emergência à maturação fisiológica, associadas ao conhecimento da fenologia da cultura, podem definir a época de plantio, evitando as conseqüências dos veranicos, a utilização de insumos, fertilizantes, inseticidas, fungicidas e herbicidas e da colheita dos grãos ou silagem. No plantio direto na palha e com a rotação de culturas é necessário manejar a época de plantio e as densidades das plantas. O consumo total de água pelas plantas de milho varia muito com o IÚvel de manejo e com a disponibilidade de água no solo. A quantidade de água necessária à planta do milho poderá ser estimada utilizando-se dados climáticos. |
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Palavras-Chave: |
Coeficiente de cultura; Disponibilidade hídrica; Ecofisiologia vegetal. |
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Thesagro: |
Evapotranspiração; Radiação Solar; Temperatura; Zea Mays. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66349/1/Exigencias-climaticas.pdf
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Marc: |
LEADER 01987naa a2200241 a 4500
001 1490276
005 2018-06-06
008 2006 bl uuuu u00u1 u #d
100 1 $aSILVA, W. J. da
245 $aExigências climáticas do milho em sistema plantio direto.$h[electronic resource]
260 $c2006
520 $aA produtividade do milho depende do número de grãos potencialmente capazes de se desenvolver, e o enchimento desses grãos, dos fatores ambientais. A intensidade com que a cultura do milho expressa seu potencial genético é determinada por sua interação com o regime de radiação solar, com a temperatura, com o déficit de pressão de vapor, com a velocidade do vento e com as características físico-hídricas do solo. Aparentemente, não existe limite máximo de temperatura para a produção de milho, no entanto, a produtividade tende a diminuir com o aumento dela. As exigências térmicas do milho, da emergência à maturação fisiológica, associadas ao conhecimento da fenologia da cultura, podem definir a época de plantio, evitando as conseqüências dos veranicos, a utilização de insumos, fertilizantes, inseticidas, fungicidas e herbicidas e da colheita dos grãos ou silagem. No plantio direto na palha e com a rotação de culturas é necessário manejar a época de plantio e as densidades das plantas. O consumo total de água pelas plantas de milho varia muito com o IÚvel de manejo e com a disponibilidade de água no solo. A quantidade de água necessária à planta do milho poderá ser estimada utilizando-se dados climáticos.
650 $aEvapotranspiração
650 $aRadiação Solar
650 $aTemperatura
650 $aZea Mays
653 $aCoeficiente de cultura
653 $aDisponibilidade hídrica
653 $aEcofisiologia vegetal
700 1 $aSANS, L. M. A.
700 1 $aMAGALHAES, P. C.
700 1 $aDURAES, F. O. M.
773 $tInforme Agropecuário, Belo Horizonte$gv. 27, n. 233, p. 14-25, jul./ago. 2006.
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