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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
28/12/2010 |
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Data da última atualização: |
20/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SEIBERT, C. H.; BORSA, M.; ROSA, R. D.; CARGNIN-FERREIRA, E.; PEREIRA, A. M. L.; GRISARD, E. C.; ZANETTI, C. R.; PINTO, A. R. |
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Afiliação: |
CAROLINE H. SEIBERT, UNIVERSIDADE FEDERAL DE SANTA CATARINA; MARIANA BORSA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; RAFAEL D. ROSA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; EDUARDO CARGNIN-FERREIRA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ALITIENE MOURA LEMOS PEREIRA, CPAMN; EDMUNDO C. GRISARD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; CARLOS R. ZANETTI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; AGUINALDO R. PINTO, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Detection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Virological Methods, Amsterdam-Holanda, v. 169, n. 1, p. 169-175, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Infectious myonecrosis virus (IMNV) has been causing a progressive disease in farm-reared shrimps in Brazil and Indonesia. Immunodiagnosticmethods for IMNVdetection, although reliable, are not employed currently becausemonoclonal antibodies (MAbs) against this virus are not available. In this study, a fragment of the IMNVmajor capsid protein gene, comprising amino acids 300?527 (IMNV300?527),was cloned and expressed in Escherichia coli. The nucleotide sequence of the recombinant IMNV300?527 fragment displayed a high degree of identity to the major capsid protein of IMNV isolates from Brazil (99%) and Indonesia (98%). Ten MAbs were generated against the expressed fragment, and eight of these, mostly IgG2a or IgG2b,were able to bind to IMNVin tissue extracts fromshrimps infected naturally in immunodotblot assays. Six of these MAbs recognized a ?100 kDa protein in a Western-blot, which is the predicted mass of IMNV major capsid protein, and also bound to viral inclusions present in muscle ?broses and in coagulativemyonecrosis, as demonstrated by immunohistochemistry. Among all thoseMAbs created, four did not cross-react with non-infected shrimp tissues; this observation supports their applicability as a sensitive and speci?c immunodiagnosis of IMNV infection in shrimps. |
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Palavras-Chave: |
Proteína recombinante; Vírus da mionecrose infecciosa. |
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Thesagro: |
Anticorpo Monoclonal; Camarão. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/24511/1/JOURNAL.pdf
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Marc: |
LEADER 02064naa a2200253 a 4500
001 1870999
005 2022-06-20
008 2010 bl uuuu u00u1 u #d
100 1 $aSEIBERT, C. H.
245 $aDetection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies.
260 $c2010
520 $aInfectious myonecrosis virus (IMNV) has been causing a progressive disease in farm-reared shrimps in Brazil and Indonesia. Immunodiagnosticmethods for IMNVdetection, although reliable, are not employed currently becausemonoclonal antibodies (MAbs) against this virus are not available. In this study, a fragment of the IMNVmajor capsid protein gene, comprising amino acids 300?527 (IMNV300?527),was cloned and expressed in Escherichia coli. The nucleotide sequence of the recombinant IMNV300?527 fragment displayed a high degree of identity to the major capsid protein of IMNV isolates from Brazil (99%) and Indonesia (98%). Ten MAbs were generated against the expressed fragment, and eight of these, mostly IgG2a or IgG2b,were able to bind to IMNVin tissue extracts fromshrimps infected naturally in immunodotblot assays. Six of these MAbs recognized a ?100 kDa protein in a Western-blot, which is the predicted mass of IMNV major capsid protein, and also bound to viral inclusions present in muscle ?broses and in coagulativemyonecrosis, as demonstrated by immunohistochemistry. Among all thoseMAbs created, four did not cross-react with non-infected shrimp tissues; this observation supports their applicability as a sensitive and speci?c immunodiagnosis of IMNV infection in shrimps.
650 $aAnticorpo Monoclonal
650 $aCamarão
653 $aProteína recombinante
653 $aVírus da mionecrose infecciosa
700 1 $aBORSA, M.
700 1 $aROSA, R. D.
700 1 $aCARGNIN-FERREIRA, E.
700 1 $aPEREIRA, A. M. L.
700 1 $aGRISARD, E. C.
700 1 $aZANETTI, C. R.
700 1 $aPINTO, A. R.
773 $tJournal of Virological Methods, Amsterdam-Holanda$gv. 169, n. 1, p. 169-175, 2010.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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| Registros recuperados : 5 | |
| 2. |  | RODRIGUES, A. P. O.; GOMINHO-ROSA, M. D. C.; CARGNIN-FERREIRA, E.; FRANCISCO, A. de; FRACALOSSI, D. M. Different utilization of plant sources by the omnivores jundiá catfish (Rhamdia quelen) and Nile tilapia (Oreochromis niloticus). Aquaculture Nutrition, Oxford, v. 18, n. 1, p. 65-72, Feb. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pesca e Aquicultura. |
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| 4. | | BORSA, M.; SEIBERT, C. H.; ROSA, R. D.; STOCO, P. H.; CARGNIN-FERREIRA, E.; PEREIRA, A. M. L.; GRISARD, E. C.; ZANETTI, C. R.; PINTO, A. R. Detection of infectious myonecrosis virus in penaeid shrimps using immunoassays: usefulness of monoclonal antibodies directed to the viral major capsid protein. Archives of Virology, New York, v. 156, n. 1, p. 9-16, jan. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio-Norte. |
|  |
| 5. | | SEIBERT, C. H.; BORSA, M.; ROSA, R. D.; CARGNIN-FERREIRA, E.; PEREIRA, A. M. L.; GRISARD, E. C.; ZANETTI, C. R.; PINTO, A. R. Detection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies. Journal of Virological Methods, Amsterdam-Holanda, v. 169, n. 1, p. 169-175, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 5 | |
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