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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
31/10/2017 |
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Data da última atualização: |
15/07/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
WERLANG, G. O.; PAIM, D. S; VIEIRA, T. R.; PISSETTI, C.; KICH, J. D.; CARDOSO, M. R. de I. |
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Afiliação: |
GABRIELA OROSCO WERLANG, UFRGS/Faculdade de Veterinária; DANIEL SANTOS PAIM, UFRGS/Faculdade de Veterinária; TATIANA REGINA VIEIRA, UFRGS/Faculdade de Veterinária; CAROLINE PISSETTI, UFRGS/Faculdade de Veterinária; JALUSA DEON KICH, CNPSA; MARIA RIBEIRO DE ITAPEMA CARDOSO, UFRGS/Faculdade de Veterinária. |
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Título: |
Detection of salmonella heidelberg resistant to colistin in the intestinal content of pigs at slaughter. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF BIOLOGICAL, CHEMICAL AND PHYSICAL HAZARDS IN PIG AND PORK, 12., 2017, Foz do Iguaçu. Proceedings Book. Concórdia: Embrapa Suínos e Aves, 2017. p. 175-179. |
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Idioma: |
Inglês |
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Notas: |
SafePork 2017. |
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Conteúdo: |
Abstract : Salmonella Heidelberg has increasingly been reported as cause of human salmonellosis worldwide. In Brazil, S. Heidelberg has been reported in poultry but it is infrequently isolated from pigs. Here, we describe the isolation of S. Heidelberg resistant to colistin from slaughter pigs. Five pigs and their carcasses belonging to a same slaughter batch in ten consecutive days were sampled for fragment of intestine in the ileocecal region and sponges rubbed on the carcass surface (400 cm2 ) before chilling. Salmonella detection was performed according to the ISO 6579:2002. Intestinal content was also subjected to Salmonella enumeration by a miniaturized Most Probable Number (MPN) protocol. Salmonella isolates were characterized by antimicrobial resistance by the disk diffusion test, the minimum inhibitory concentration to colistin determination and to gene mcr-1 investigation by PCR. Salmonella was isolated from the intestinal content of 64% (32/50) of the pigs, in amounts that varied from 2.7 to >1,400 MPN/g. Salmonella Heidelberg was the most frequent serovar identified in the intestinal content samples (20/50; 40%), and this serovar was present in eight of the ten pig batches sampled. At the prechill, Salmonella was isolated from 8% of carcasses, and S. Heidelberg was not detected. Salmonella Heidelberg strains were resistant against ampicillin (n=9), tetracycline (n=8), sulfonamide (n=8) and gentamicin (n=5). Nine multi-drug resistant strains were detected; among them four strains were positive for the gene mcr-1. In these strains the MIC value was 8 µg.mL-1 , while in the strains without the mcr-1 gene it ranged from 2 µg.mL-1 to 4 µg.mL-1 . Therefore, humans in contact with carrier pigs or their environment may be exposed to S. Heidelberg, including strains harboring the gene mcr-1. MenosAbstract : Salmonella Heidelberg has increasingly been reported as cause of human salmonellosis worldwide. In Brazil, S. Heidelberg has been reported in poultry but it is infrequently isolated from pigs. Here, we describe the isolation of S. Heidelberg resistant to colistin from slaughter pigs. Five pigs and their carcasses belonging to a same slaughter batch in ten consecutive days were sampled for fragment of intestine in the ileocecal region and sponges rubbed on the carcass surface (400 cm2 ) before chilling. Salmonella detection was performed according to the ISO 6579:2002. Intestinal content was also subjected to Salmonella enumeration by a miniaturized Most Probable Number (MPN) protocol. Salmonella isolates were characterized by antimicrobial resistance by the disk diffusion test, the minimum inhibitory concentration to colistin determination and to gene mcr-1 investigation by PCR. Salmonella was isolated from the intestinal content of 64% (32/50) of the pigs, in amounts that varied from 2.7 to >1,400 MPN/g. Salmonella Heidelberg was the most frequent serovar identified in the intestinal content samples (20/50; 40%), and this serovar was present in eight of the ten pig batches sampled. At the prechill, Salmonella was isolated from 8% of carcasses, and S. Heidelberg was not detected. Salmonella Heidelberg strains were resistant against ampicillin (n=9), tetracycline (n=8), sulfonamide (n=8) and gentamicin (n=5). Nine multi-drug resistant strains were detected; among t... Mostrar Tudo |
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Palavras-Chave: |
Safe pork. |
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Thesagro: |
Abate; Salmonella; Sanidade animal; Suíno. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/165883/1/final8693.pdf
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Marc: |
LEADER 02699nam a2200241 a 4500
001 2078570
005 2019-07-15
008 2017 bl uuuu u00u1 u #d
100 1 $aWERLANG, G. O.
245 $aDetection of salmonella heidelberg resistant to colistin in the intestinal content of pigs at slaughter.$h[electronic resource]
260 $aIn: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF BIOLOGICAL, CHEMICAL AND PHYSICAL HAZARDS IN PIG AND PORK, 12., 2017, Foz do Iguaçu. Proceedings Book. Concórdia: Embrapa Suínos e Aves, 2017. p. 175-179.$c2017
500 $aSafePork 2017.
520 $aAbstract : Salmonella Heidelberg has increasingly been reported as cause of human salmonellosis worldwide. In Brazil, S. Heidelberg has been reported in poultry but it is infrequently isolated from pigs. Here, we describe the isolation of S. Heidelberg resistant to colistin from slaughter pigs. Five pigs and their carcasses belonging to a same slaughter batch in ten consecutive days were sampled for fragment of intestine in the ileocecal region and sponges rubbed on the carcass surface (400 cm2 ) before chilling. Salmonella detection was performed according to the ISO 6579:2002. Intestinal content was also subjected to Salmonella enumeration by a miniaturized Most Probable Number (MPN) protocol. Salmonella isolates were characterized by antimicrobial resistance by the disk diffusion test, the minimum inhibitory concentration to colistin determination and to gene mcr-1 investigation by PCR. Salmonella was isolated from the intestinal content of 64% (32/50) of the pigs, in amounts that varied from 2.7 to >1,400 MPN/g. Salmonella Heidelberg was the most frequent serovar identified in the intestinal content samples (20/50; 40%), and this serovar was present in eight of the ten pig batches sampled. At the prechill, Salmonella was isolated from 8% of carcasses, and S. Heidelberg was not detected. Salmonella Heidelberg strains were resistant against ampicillin (n=9), tetracycline (n=8), sulfonamide (n=8) and gentamicin (n=5). Nine multi-drug resistant strains were detected; among them four strains were positive for the gene mcr-1. In these strains the MIC value was 8 µg.mL-1 , while in the strains without the mcr-1 gene it ranged from 2 µg.mL-1 to 4 µg.mL-1 . Therefore, humans in contact with carrier pigs or their environment may be exposed to S. Heidelberg, including strains harboring the gene mcr-1.
650 $aAbate
650 $aSalmonella
650 $aSanidade animal
650 $aSuíno
653 $aSafe pork
700 1 $aPAIM, D. S
700 1 $aVIEIRA, T. R.
700 1 $aPISSETTI, C.
700 1 $aKICH, J. D.
700 1 $aCARDOSO, M. R. de I.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
01/03/2016 |
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Data da última atualização: |
13/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVERIO, M. S.; DONATONI, F. A. B.; RUSCITO, M. M.; JUNGMANN, L.; VIGNA, B. B. Z. |
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Afiliação: |
MANUELA S. SILVERIO, UNIVERSIDADE FEDERAL DE UBERLÂNDIA; FLAVIA ALINE BRESSANI DONATONI, CPPSE; MÔNICA MASCARO RUSCITO, UNIARA - CNPq; LETICIA JUNGMANN CANCADO, CNPGC; BIANCA BACCILI ZANOTTO VIGNA, CPPSE. |
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Título: |
Microsatellite transferability from Brachiaria ruziziensis to B. humidicola and genetic similarity among evaluated genotypes. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM OF FORAGE BREEDING, 5., 2015, Buenos Aires, Argentina. Proceedings... Buenos Aires, Argentina: Facultad de Agronomía, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Species from the genus Brachiaria play an important role in tropical pastures. Brachiaria ruziziensis presents di and tetraploid cytotypes and B. humidicola presents from tetra to heptaploid cytotypes. Although they belong to the same genus, these species belong to different agamic complexes. |
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Palavras-Chave: |
B humidicola. |
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Thesagro: |
Brachiaria Ruziziensis. |
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Thesaurus NAL: |
Urochloa ruziziensis. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140463/1/Silverio.pdf
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Marc: |
LEADER 01029nam a2200193 a 4500
001 2039255
005 2023-06-13
008 2015 bl uuuu u00u1 u #d
100 1 $aSILVERIO, M. S.
245 $aMicrosatellite transferability from Brachiaria ruziziensis to B. humidicola and genetic similarity among evaluated genotypes.$h[electronic resource]
260 $aIn: INTERNATIONAL SYMPOSIUM OF FORAGE BREEDING, 5., 2015, Buenos Aires, Argentina. Proceedings... Buenos Aires, Argentina: Facultad de Agronomía$c2015
520 $aSpecies from the genus Brachiaria play an important role in tropical pastures. Brachiaria ruziziensis presents di and tetraploid cytotypes and B. humidicola presents from tetra to heptaploid cytotypes. Although they belong to the same genus, these species belong to different agamic complexes.
650 $aUrochloa ruziziensis
650 $aBrachiaria Ruziziensis
653 $aB humidicola
700 1 $aDONATONI, F. A. B.
700 1 $aRUSCITO, M. M.
700 1 $aJUNGMANN, L.
700 1 $aVIGNA, B. B. Z.
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Embrapa Pecuária Sudeste (CPPSE) |
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