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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
23/08/2018 |
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Data da última atualização: |
23/08/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
GENRO, T. C. M.; PRATES, E. R.; CARDOSO, F. F.; SAN'THIAGO, L. R. L. |
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Afiliação: |
TERESA CRISTINA MORAES GENRO, CPPSUL; UFRGS; FERNANDO FLORES CARDOSO, CPPSUL. |
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Título: |
Diet selection in a pasture of Panicum maximum cv. Mombaça with different sward heights. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
In: INTERNATIONAL GRASSLAND CONGRESS, 20., 2005, Dublin, Irland. Offered paper... Dublin: Wageningen Academic, 2005. |
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Páginas: |
p. 505. |
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Idioma: |
Inglês |
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Conteúdo: |
Resumo. |
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Thesagro: |
Dieta; Pastagem; Ruminante. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181871/1/Genro-et-al-2005.pdf
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Marc: |
LEADER 00634nam a2200193 a 4500
001 2094456
005 2018-08-23
008 2005 bl uuuu u00u1 u #d
100 1 $aGENRO, T. C. M.
245 $aDiet selection in a pasture of Panicum maximum cv. Mombaça with different sward heights.$h[electronic resource]
260 $aIn: INTERNATIONAL GRASSLAND CONGRESS, 20., 2005, Dublin, Irland. Offered paper... Dublin: Wageningen Academic$c2005
300 $ap. 505.
520 $aResumo.
650 $aDieta
650 $aPastagem
650 $aRuminante
700 1 $aPRATES, E. R.
700 1 $aCARDOSO, F. F.
700 1 $aSAN'THIAGO, L. R. L.
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Registro original: |
Embrapa Pecuária Sul (CPPSUL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/02/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREIRA, M. M.; RAPOSO, N. R. B.; BRAYNER, R.; TEIXEIRA, E. M.; OLIVEIRA, V.; QUINTAO, C. C. R.; CAMARGO, L. S. de A.; MATTOSO, L. H. C.; BRANDAO, H. de M. |
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Afiliação: |
M. M. PEREIRA, UFJF; N. R. B. RAPOSO, UFJF; R. BRAYNER, University of Paris Diderot, Paris; E. M. TEIXEIRA; V. OLIVEIRA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA; HUMBERTO DE MELLO BRANDAO, CNPGL. |
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Título: |
Cytotoxicity and expression of genes involved in the cellular stress response and apoptosis in mammalian fibroblast exposed to cotton cellulose nanofibers. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Nanotechnology, v. 24, n. 7, 075103, 2013. |
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DOI: |
https://doi.org/10.1088/0957-4484/24/7/075103 |
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Idioma: |
Inglês |
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Conteúdo: |
Cellulose nanofibers (CNF) have mechanical properties that make them very attractive for applications in the construction of polymeric matrices, drug delivery and tissue engineering. However, little is known about their impact on mammalian cells. The objective of this study was to evaluate the cytotoxicity of CNF and their effect on gene expression of fibroblasts cultured in vitro. The morphology of CNF was analyzed by transmission electron microscopy and the surface charge by Zeta potential. Cell viability was analyzed by flow cytometry assay and gene expression of biomarkers focused on cell stress response such as Heat shock protein 70.1 (HSP70.1) and Peroxiredoxin 1 (PRDX1) and apoptosis as B-cell leukemia (BCL-2) and BCL-2 associated X protein (BAX) by RT-PCR assay. Low concentrations of CNF (0.02-100 μg ml(-1)) did not cause cell death; however, at concentrations above 200 μg ml(-1), the nanofibers significantly decreased cell viability (86.41 ± 5.37%). The exposure to high concentrations of CNF (2000 and 5000 μg ml(-1)) resulted in increased HSP70.1, PRDX1 and BAX gene expression. The current study concludes that, under the conditions tested, high concentrations (2000 and 5000 μg ml(-1)) of CNF cause decreased cell viability and affect the expression of stress- and apoptosis-associated molecular markers. |
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Palavras-Chave: |
Animals Apoptosis; Apoptosis/genetics; Cattle Cellulose/pharmacology; Drug effects; Fibroblasts/cytology; Fibroblasts/drug. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02323naa a2200301 a 4500
001 1979437
005 2024-02-05
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1088/0957-4484/24/7/075103$2DOI
100 1 $aPEREIRA, M. M.
245 $aCytotoxicity and expression of genes involved in the cellular stress response and apoptosis in mammalian fibroblast exposed to cotton cellulose nanofibers.$h[electronic resource]
260 $c2013
520 $aCellulose nanofibers (CNF) have mechanical properties that make them very attractive for applications in the construction of polymeric matrices, drug delivery and tissue engineering. However, little is known about their impact on mammalian cells. The objective of this study was to evaluate the cytotoxicity of CNF and their effect on gene expression of fibroblasts cultured in vitro. The morphology of CNF was analyzed by transmission electron microscopy and the surface charge by Zeta potential. Cell viability was analyzed by flow cytometry assay and gene expression of biomarkers focused on cell stress response such as Heat shock protein 70.1 (HSP70.1) and Peroxiredoxin 1 (PRDX1) and apoptosis as B-cell leukemia (BCL-2) and BCL-2 associated X protein (BAX) by RT-PCR assay. Low concentrations of CNF (0.02-100 μg ml(-1)) did not cause cell death; however, at concentrations above 200 μg ml(-1), the nanofibers significantly decreased cell viability (86.41 ± 5.37%). The exposure to high concentrations of CNF (2000 and 5000 μg ml(-1)) resulted in increased HSP70.1, PRDX1 and BAX gene expression. The current study concludes that, under the conditions tested, high concentrations (2000 and 5000 μg ml(-1)) of CNF cause decreased cell viability and affect the expression of stress- and apoptosis-associated molecular markers.
653 $aAnimals Apoptosis
653 $aApoptosis/genetics
653 $aCattle Cellulose/pharmacology
653 $aDrug effects
653 $aFibroblasts/cytology
653 $aFibroblasts/drug
700 1 $aRAPOSO, N. R. B.
700 1 $aBRAYNER, R.
700 1 $aTEIXEIRA, E. M.
700 1 $aOLIVEIRA, V.
700 1 $aQUINTAO, C. C. R.
700 1 $aCAMARGO, L. S. de A.
700 1 $aMATTOSO, L. H. C.
700 1 $aBRANDAO, H. de M.
773 $tNanotechnology$gv. 24, n. 7, 075103, 2013.
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Embrapa Gado de Leite (CNPGL) |
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