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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/01/2011 |
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Data da última atualização: |
18/06/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RUSSO, M. S. T.; CARDINALI, M.; MILORI, D. M. B. P.; PEREIRA, F. M. V.; MARTINS, P. K.; FREITAS-ASTÚA, J. |
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Afiliação: |
DEBORA MARCONDES BASTOS P MILORI, CNPDIA. |
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Título: |
Study of laser-induced fluorescence imaging in asymptomatic leaves from citrus plants inoculated with candidatus liberibacter. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Citrus research & technology, Cordeirópolis, v. 31, suplemento, p. 122, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus greening or huanglongbing (HLB) is a devastating disease that has been causing serious problems to citrus cultivation. Some analytical methods have been reported for diagnosis of this disease, mainly DNA-based techniques of Candidatus Liberibacter species, which are the causal agents. In general, most of these developed methods involve PCR (polymerase chain reaction). However, methods based on PCR cannot be used in large-scale to build disease distribution maps. The visual inspection, usually adopted, can lead to misleading diagnoses, since the symptoms are easily mistaken with others problems and the disease has a long asymptomatic phase. Aiming the development of a new method for early diagnosis of this malady, leaves from inoculated and healthy (control group) plants were assessed using laser- induced fluorescence imaging (LIFI). The plants were measured monthly and the evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. A laser emitting at 470 nm was used to excite the fluorescence of chlorophyll and other metabolites present on the leaf. Each image was associated to histograms of colours (colourgrams) and a matrix. For the investigations of data, principal components analysis were made for the categories of the colourgram (scales of red, blue and green, luminosity, relative red, blue and green, hue, saturation and intensity). Preliminary results showed that there are differences in the images obtained from healthy and inoculated plants, indicating a great potential of the technique to perform early diagnoses in large scale that are economically viable. MenosCitrus greening or huanglongbing (HLB) is a devastating disease that has been causing serious problems to citrus cultivation. Some analytical methods have been reported for diagnosis of this disease, mainly DNA-based techniques of Candidatus Liberibacter species, which are the causal agents. In general, most of these developed methods involve PCR (polymerase chain reaction). However, methods based on PCR cannot be used in large-scale to build disease distribution maps. The visual inspection, usually adopted, can lead to misleading diagnoses, since the symptoms are easily mistaken with others problems and the disease has a long asymptomatic phase. Aiming the development of a new method for early diagnosis of this malady, leaves from inoculated and healthy (control group) plants were assessed using laser- induced fluorescence imaging (LIFI). The plants were measured monthly and the evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. A laser emitting at 470 nm was used to excite the fluorescence of chlorophyll and other metabolites present on the leaf. Each image was associated to histograms of colours (colourgrams) and a matrix. For the investigations of data, principal components analysis were made for the categories of the colourgram (scales of red, blue and green, luminosity, relative red, blue and green, hue, saturation and intensity). Preliminary results showed that there are differences in ... Mostrar Tudo |
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Palavras-Chave: |
Evento; Huanglongbing; Plant disease. |
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Thesagro: |
Bactéria; Doença de Planta; Fruta Cítrica; Greening. |
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Thesaurus Nal: |
Candidatus Liberibacter; Citrus. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84535/1/Proci-10.00257.pdf
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Marc: |
LEADER 02611nam a2200277 a 4500
001 1881236
005 2013-06-18
008 2010 bl uuuu u00u1 u #d
100 1 $aRUSSO, M. S. T.
245 $aStudy of laser-induced fluorescence imaging in asymptomatic leaves from citrus plants inoculated with candidatus liberibacter.
260 $aCitrus research & technology, Cordeirópolis, v. 31, suplemento, p. 122, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas.$c2010
520 $aCitrus greening or huanglongbing (HLB) is a devastating disease that has been causing serious problems to citrus cultivation. Some analytical methods have been reported for diagnosis of this disease, mainly DNA-based techniques of Candidatus Liberibacter species, which are the causal agents. In general, most of these developed methods involve PCR (polymerase chain reaction). However, methods based on PCR cannot be used in large-scale to build disease distribution maps. The visual inspection, usually adopted, can lead to misleading diagnoses, since the symptoms are easily mistaken with others problems and the disease has a long asymptomatic phase. Aiming the development of a new method for early diagnosis of this malady, leaves from inoculated and healthy (control group) plants were assessed using laser- induced fluorescence imaging (LIFI). The plants were measured monthly and the evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. A laser emitting at 470 nm was used to excite the fluorescence of chlorophyll and other metabolites present on the leaf. Each image was associated to histograms of colours (colourgrams) and a matrix. For the investigations of data, principal components analysis were made for the categories of the colourgram (scales of red, blue and green, luminosity, relative red, blue and green, hue, saturation and intensity). Preliminary results showed that there are differences in the images obtained from healthy and inoculated plants, indicating a great potential of the technique to perform early diagnoses in large scale that are economically viable.
650 $aCandidatus Liberibacter
650 $aCitrus
650 $aBactéria
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aGreening
653 $aEvento
653 $aHuanglongbing
653 $aPlant disease
700 1 $aCARDINALI, M.
700 1 $aMILORI, D. M. B. P.
700 1 $aPEREIRA, F. M. V.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS-ASTÚA, J.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
09/03/2023 |
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Data da última atualização: |
23/08/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
AMORIM, M. R.; SILVA, V. R. C. da; CUNHA, M. A. C. da; OLIVEIRA, C. R. S. de; LEAO, P. C. de S. |
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Afiliação: |
MARILÚCIA RIBEIRO AMORIM, Estagiária da UPE; VITÓRIA RAMOS CRUZ DA SILVA, Bolsista Pibic; MARCOS ANDREI CUSTODIO DA CUNHA, Bolsista Capes; CARLOS ROBERTO SILVA DE OLIVEIRA, Bolsista Capes; PATRICIA COELHO DE SOUZA LEAO, CPATSA. |
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Título: |
Diversidade genética entre cultivares de uvas para processamento do Banco de Germoplasma de videira da Embrapa Semiárido. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 16., 2022, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2022. |
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Páginas: |
p. 37. |
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Série: |
(Embrapa Semiárido. Documentos, 308). |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi estudar a diversidade genética existente entre as cultivares de uvas para processamento de vinhos e sucos, avaliadas no ciclo de produção do segundo semestre de 2021, no Banco de Germoplasma (BAG) de Videira da Embrapa Semiárido. |
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Palavras-Chave: |
Cultivares de videira; Diversidade genética; Recursos genéticos; Uva de mesa. |
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Thesagro: |
Germoplasma; Melhoramento Genético Vegetal; Suco; Uva; Vinho. |
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Thesaurus NAL: |
Grapes. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152215/1/Diversidade-genetica-entre-cultivares-de-uvas-2022.pdf
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Marc: |
LEADER 01261nam a2200301 a 4500
001 2152215
005 2023-08-23
008 2022 bl uuuu u00u1 u #d
100 1 $aAMORIM, M. R.
245 $aDiversidade genética entre cultivares de uvas para processamento do Banco de Germoplasma de videira da Embrapa Semiárido.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 16., 2022, Petrolina. Anais... Petrolina: Embrapa Semiárido$c2022
300 $ap. 37.
490 $a(Embrapa Semiárido. Documentos, 308).
520 $aO objetivo deste trabalho foi estudar a diversidade genética existente entre as cultivares de uvas para processamento de vinhos e sucos, avaliadas no ciclo de produção do segundo semestre de 2021, no Banco de Germoplasma (BAG) de Videira da Embrapa Semiárido.
650 $aGrapes
650 $aGermoplasma
650 $aMelhoramento Genético Vegetal
650 $aSuco
650 $aUva
650 $aVinho
653 $aCultivares de videira
653 $aDiversidade genética
653 $aRecursos genéticos
653 $aUva de mesa
700 1 $aSILVA, V. R. C. da
700 1 $aCUNHA, M. A. C. da
700 1 $aOLIVEIRA, C. R. S. de
700 1 $aLEAO, P. C. de S.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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