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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
05/11/2007 |
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Data da última atualização: |
31/07/2018 |
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Autoria: |
SILVEIRA, E. L. da; PEREIRA, R. M.; SCAQUITTO, D. C.; PEDRINHO, E. A. N.; VAL-MORAES, S.P.; WICKERT, E.; CARARETO-ALVES, L.M.; LEMOS, E. G. de M. |
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Afiliação: |
Érico Leandro da Silveira, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia; Rodrigo Matheus Pereira, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia; Denilson César Scaquitto, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia; Eliamar Aparecida Nascimbém Pedrinho, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia; Silvana Pómpeia Val-Moraes, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia; Ester Wickert, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia; Lúcia Maria Carareto-Alves, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia; Eliana Gertrudes de Macedo Lemos, Universidade Estadual Paulista - Unesp/Faculdade de Ciências Agrárias e Veterinárias/Departamento de Tecnologia. |
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Título: |
Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 10, p. 1507-1516, out. 2006 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Diversidade bacteriana de solo sob eucaliptos obtida por seqüenciamento do 16S rDNA. |
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Conteúdo: |
Studies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (NFA) and an eucalyptus arboretum (EAA). PCR primers, whose target soil metagenomic 16S rDNA were used to amplify soil DNA, were cloned using pGEM-T and sequenced to determine bacterial diversity. From the NFA soil 134 clones were analyzed, while 116 clones were analyzed from the EAA soil samples. The sequences were compared with those online at the GenBank. Phylogenetic analyses revealed differences between the soil types and high diversity in both communities. Soil from the Eucalyptus spp. arboretum was found to have a greater bacterial diversity than the soil investigated from the native forest area. |
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Palavras-Chave: |
comunidades microbianas; diversidade genética; genetic diversity; metagenomic; metagenômica. |
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Thesaurus Nal: |
microbial communities. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107031/1/Bacterial.pdf
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Marc: |
LEADER 02147naa a2200289 a 4500
001 1121634
005 2018-07-31
008 2006 bl uuuu u00u1 u #d
100 1 $aSILVEIRA, E. L. da
245 $aBacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis.
260 $c2006
500 $aTítulo em português: Diversidade bacteriana de solo sob eucaliptos obtida por seqüenciamento do 16S rDNA.
520 $aStudies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (NFA) and an eucalyptus arboretum (EAA). PCR primers, whose target soil metagenomic 16S rDNA were used to amplify soil DNA, were cloned using pGEM-T and sequenced to determine bacterial diversity. From the NFA soil 134 clones were analyzed, while 116 clones were analyzed from the EAA soil samples. The sequences were compared with those online at the GenBank. Phylogenetic analyses revealed differences between the soil types and high diversity in both communities. Soil from the Eucalyptus spp. arboretum was found to have a greater bacterial diversity than the soil investigated from the native forest area.
650 $amicrobial communities
653 $acomunidades microbianas
653 $adiversidade genética
653 $agenetic diversity
653 $ametagenomic
653 $ametagenômica
700 1 $aPEREIRA, R. M.
700 1 $aSCAQUITTO, D. C.
700 1 $aPEDRINHO, E. A. N.
700 1 $aVAL-MORAES, S.P.
700 1 $aWICKERT, E.
700 1 $aCARARETO-ALVES, L.M.
700 1 $aLEMOS, E. G. de M.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 41, n. 10, p. 1507-1516, out. 2006
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Embrapa Unidades Centrais (AI-SEDE) |
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