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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
14/10/2015 |
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Data da última atualização: |
18/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GAVA, D.; SOUZA, C. K.; SCHAEFER, R.; VINCENT, A. L.; CANTAO, M. E.; COLDEBELLA, A.; ZANELLA, J. R. C. |
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Afiliação: |
DANIELLE GAVA, CNPSA; CARINE KUNZLER DE SOUZA, UFRGS; REJANE SCHAEFER, CNPSA; AMY LOUISE VINCENT, USDA/ARS; MAURICIO EGIDIO CANTAO, CNPSA; ARLEI COLDEBELLA, CNPSA; JANICE REIS CIACCI ZANELLA, CNPSA. |
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Título: |
A TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 219, p. 14-17, 2015. |
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DOI: |
http://dx.doi.org/10.1016/j.jviromet.2015.03.011 |
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Idioma: |
Inglês |
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Conteúdo: |
Porcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 101 DNA copies/L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98?99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds. |
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Palavras-Chave: |
Porcine parvovirus; Swine disease. |
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Thesagro: |
Doença animal; Parvovirose; Suíno; Virologia. |
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Thesaurus Nal: |
Parvoviridae; Quantitative polymerase chain reaction; SsDNA viruses; Virology. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02164naa a2200325 a 4500
001 2026395
005 2016-02-18
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1016/j.jviromet.2015.03.011$2DOI
100 1 $aGAVA, D.
245 $aA TaqMan-based real-time PCR for detection and quantification of porcine parvovirus 4.$h[electronic resource]
260 $c2015
520 $aPorcine parvovirus 4 (PPV4) is a DNA virus, and a member of the Parvoviridae family within the Bocavirus genera. It was detected recently in swine, but its epidemiology and pathology remain unclear. A TaqManbased real-time PCR (qPCR) targeting a conserved region of the ORF3 gene of PPV4 was developed. The qPCR detection limit was 9.5 × 101 DNA copies/L. There was no cross-reaction with porcine parvovirus, torque teno virus 1, torque teno virus 2, porcine circovirus type 1, porcine circovirus type 2, or with pseudorabies virus. Two hundred and seventy-two samples, including serum, semen, lungs, feces, ovarian follicular fluids, ovaries and uterus, were evaluated by qPCR and PPV4 was detected in 36 samples (13.2%). When compared with a conventional PCR (cPCR), the qPCR assay was 10 times more sensitive and the detection of PPV4 DNA in field samples was increased 2.5 times. Partial sequencing of PPV4 ORF3 gene, obtained from two pooled samples of uterus and ovaries, revealed a high nucleotide identity (98?99%) with a reference PPV4 sequence. The qPCR can be used as a fast and accurate assay for the detection and quantification of PPV4 in field samples and for epidemiological studies in swine herds.
650 $aParvoviridae
650 $aQuantitative polymerase chain reaction
650 $aSsDNA viruses
650 $aVirology
650 $aDoença animal
650 $aParvovirose
650 $aSuíno
650 $aVirologia
653 $aPorcine parvovirus
653 $aSwine disease
700 1 $aSOUZA, C. K.
700 1 $aSCHAEFER, R.
700 1 $aVINCENT, A. L.
700 1 $aCANTAO, M. E.
700 1 $aCOLDEBELLA, A.
700 1 $aZANELLA, J. R. C.
773 $tJournal of Virological Methods$gv. 219, p. 14-17, 2015.
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Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 147 | |
| 5. |  | SILVA, M. L. B. da; CANTAO, M. E.; MEZZARI, M. P.; MA, J.; NOSSA, C. W. Assessment of bacterial and archaeal community structure in swine wastewater treatment processes. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 4., 2015, Rio de Janeiro, RJ. Anais... Brasília: Embrapa, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 8. | | SCHAEFER, R.; GAVA, D.; HAACH, V.; CANTAO, M. E.; ZANELLA, J. R. C. Continuous circulation of human-origin seasonal influenza A viruses in swine in Brazil. Virus Reviews & Research, Brasília, DF, v. 20, p. 242, Oct. 2015. Supplement 1, ref. VV301. Edição dos Resumos do XXVI Brazilian Congress of Virology, X Mercosur Meeting of Virology, Florianóplis, SC, Oct. 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 14. | | PEIXOTO, J. de O.; IBELLI, A. M. G.; CANTAO, M. E.; LEDUR, M. C. Condronecrose bacteriana com osteomielite (BCO): um problema subestimado na avicultura de corte. Guia Gessulli da Avicultura e Suinocultura Industrial, Itu, ed. 266, ano 38, n. 5, p. 24-30, 2015.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 15. | | TUPINAMBA, D.; CANTAO, M. E.; COSTA, O. Y.; BERGMANN, J. C.; KYAW, C.; QUIRINO, B. F. Impact of oil palm fatal yellowing on Amazon soil archaeal community assessed by high-throughput sequencing. In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 37., 2015, San Diego, California. [Resumos ...] Fairfax: Society for Industrial Microbiology and Biotechnology, 2015. Resumo T141| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | SCHAEFER, R.; GAVA, D.; NELSON, M. I.; HAACH, V.; ZANELLA, J. R. C.; CANTAO, M. E. Genetic characterization of influenza viruses circulating within Brazilian swine between 2009 and 2016. In: BRAZILIAN CONGRESS OF VIROLOGY, 27.; MERCOSUR MEETING OF VIROLOGY,11., 2016, Pirenópolis. Abstracts: posters: oral presentation. Brasília, DF: SBV, 2016. Publicado na Virus Reviews and Research, Brasília, n. 20, v. 2, p. 22-23, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 18. | | NELSON, M. I.; SCHAEFER, R.; GAVA, D.; CANTAO, M. E.; ZANELLA, J. R. C. Influenza A viruses of human origin in swine, Brazil. Emerging Infectious Diseases, v. 21, n. 8, p. 1339 - 1347, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 19. | | ZANELLA, J. R. C.; SCHAEFER, R.; GAVA, D.; HAACH, V.; CANTAO, M. E.; COLDEBELLA, A. Influenza A virus infection in Brazilian swine herds following the introduction of pandemic 2009 H1N1. Veterinary Microbiology, v. 180, n. 1-2, p. 118-122, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 20. | | SCHAEFER, R.; MORES, N.; GAVA, D.; CANTAO, M. E.; ZANELLA, J. R. C. Long-term circulation of influenza A viruses in swine in Brazil. In: Journal of the Brazilian Society for Virology, Ribeirão Preto, v. 19, p. 53-54, 2014. Suppl. 2. Edição dos anais do 25º. Brazilian Congress of Virology e 9º. Mercosur Meeting of Virology, Ribeirão Preto, set./out, 2014.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 147 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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