Data/hora: 02/06/2024 17:34:50

Biblioteca(s): | Embrapa Suínos e Aves.
Data corrente: | 30/10/2020
Data da última atualização: | 30/10/2020
Autoria: | SILVEIRA, D. D.; BELLI FILHO, P.; PHILIPPI, L. S.; CANTAO, M. E.; FOULQUIER, A.; BAYLE, S.; DELFORNO, T. P.; MOLLE, P.
Afiliação: | UFSC; PAULO BELLI FILHO, UFSC; UFSC; MAURICIO EGIDIO CANTAO, CNPSA; Univ. Savoie Mont Blanc Grenoble; Univ. Montpellier; UNICAMP; INRAE.
Título: | In-depth assessment of microbial communities in the full-scale vertical flow treatment wetlands fed with raw domestic wastewater.
Ano de publicação: | 2020
Fonte/Imprenta: | Environmental Technology, 09 Feb 2020
DOI: | https://doi.org/10.1080/09593330.2020.1723709
Idioma: | Inglês
Conteúdo: | ABSTRACT A multiphase study was proposed to examine microbial communities linked to the nitrogen cycle in the first stage of four full-scale French vertical flow treatment systems. To this end, denaturing gradient gel electrophoresis (DGGE) was performed for structural assessment and quantitative PCR (qPCR) to enumerate the abundance of ammonia-oxidizing (AOB). 16S rRNA sequencing was used to assess the taxonomic profile followed by putative assessment of functional genes. The samples were collected under different conditions, such as operational time (presence/absence of sludge layer on the surface of the filters), season (winter and summer), sampling depth (0, 15 and 30 cm) and operation cycle (rest and feed periods). A structural disparity was noted in the upper layers, whereas higher similarity at 30 cm was observed highlighting the effect of organic matter on bacterial diversity. The 7th rest day was highlighted by an apparent decline in the microbial community abundance. Additionally, qPCR indicated that the largest amount of AOB was found at 30 cm depth and during the feeding period. From the taxonomic profile, Mycobacterium, Acinetobacter, Flavobacterium, and Nitrospira were the most abundant genre found in all systems. The functional prediction results showed predicted genes linked to the denitrification process. The results suggested that operating time and season were responsible for the pattern of the microbial community behavior. This study allowed us to further understand the bacterial dynamics and to advance the idea of design modifications made in the first stage of the classical French system to improve nitrogen removal are promising.
Palavras-Chave: | Comunidades microbianas; French VF wetland; Functional prediction; Microbial community structure; QPCR; Remoção de nitrogênio; Taxonomic profile.
Thesagro: | Desnitrificação; Taxonomia.
Thesaurus Nal: | Nitrifying bacteria.
Categoria do assunto: | --
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (21952-1) - UPC - AP - DD -  -  -  - DIS