Data/hora: 02/06/2024 18:34:14

Biblioteca(s): | Embrapa Suínos e Aves.
Data corrente: | 01/11/2019
Data da última atualização: | 01/11/2019
Autoria: | HAACH, V.; GAVA, D.; CANTAO, M. E.; FRANCO, A. C.
Afiliação: | VANESSA HAACH, UFRGS; DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; ANA CLÁUDIA FRANCO, UFRGS.
Título: | One-step multiplex RT-qPCR for the detection and subtyping of influenza A virus in swine in Brazil.
Ano de publicação: | 2019
Fonte/Imprenta: | Journal of Virological Methods, v. 269, p. 43-48, 2019.
DOI: | 10.1016/j.jviromet.2019.04.005
Idioma: | Inglês
Conteúdo: | Abstract: Pandemic H1N1, human-like H1N2 and H3N2 influenza A (IAV) viruses are co-circulating in swine herds in Brazil. The genetic analysis of the Brazilian IAVs has shown that they are genetically distinct from viruses found in swine in other countries; therefore, an update of the diagnostic assays for IAV detection and subtyping is needed. This study describes the development and validation of a TaqMan based ? one-step multiplex RT-qPCR to discriminate the hemagglutinin and neuraminidase genes of the three major IAV ubtypes circulating in pigs in Brazil. The RT-qPCR assays presented 100% (95.7?100, CI 95%) of diagnostic sensitivity in the analysis of 85 IAVs, previously characterized by sequencing. The limits of detection ranged from 5.09 × 101 to 5.09 × 103 viral RNA copies/&#956;L. For the analytical specificity, 73 pig samples collected during 2017 and 2018 were analyzed, resulting in the identification of the subtype in 74.0% (62.9?82.7, CI 95%) of samples. From these, 46.3% were H3N2, 33.3% were H1N1, 11.1% were H1N2 and 3.7% were HxN1. Mixed viral infections (3.7%) and reassortant viruses (1.9%) were also detected by the test. This multiplex RT-qPCR assay provides a fast and specific diagnostic tool for identification of different subtypes and lineages of IAV in pigs, contributing to the monitoring of influenza in swine.
Palavras-Chave: | Diagnóstico rápido; Multiplex RT-qPCR; Rapid diagnosis; RT-qPCR multiplex; Subtipagem; Subtyping; Vírus da gripe A.
Thesagro: | Suíno.
Thesaurus Nal: | Influenza A virus; Swine.
Categoria do assunto: | --
Registro original: | Embrapa Suínos e Aves (CNPSA)
Exemplares: | CNPSA - (21677-1) - UPC - AP - DD -  -  -  - DIS