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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
07/05/2013 |
Data da última atualização: |
17/01/2024 |
Autoria: |
LIMA-VERDE, I. B.; ROSSETTO, R.; MATOS, M. H. T.; CELESTINO, J. J. H.; BUENO, J. B.; SILVA, C. M. G.; FAUSTINO, L. R.; MORORÓ, M. B. S.; ARAÚJO, V. T.; CAMPELLO, C. C.; FIGUEIREDO, J. R. |
Título: |
Androstenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Animal Reproduction, Belo Horizonte, v. 7, n. 2, p. 80-89, Apr./Jun. 2010. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition. MenosAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of andr... Mostrar Tudo |
Palavras-Chave: |
Esteroidogenese; Foliculo pre-antral; FSH. |
Thesagro: |
Caprino; Cultura in vitro; Endocrinologia; Hormônio; Ovário; Reprodução animal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03032naa a2200349 a 4500 001 1957239 005 2024-01-17 008 2010 bl uuuu u00u1 u #d 100 1 $aLIMA-VERDE, I. B. 245 $aAndrostenedione and follicle stimulating hormone involvement in the viability and development of goat preantral follicles in vitro.$h[electronic resource] 260 $c2010 520 $aAbstract: This study investigated the effects of androstenedione and follicle-stimulating hormone (FSH) on the viability and growth of caprine preantral follicles. Ovarian tissues were cultured for 1 or 7 days in Minimum Essential Medium (MEM+) containing androstenedione (0, 1, 10, 50, or 100 ng/ml), FSH (50 ng/ml), or a combination of these two hormones. Cultured and noncultured control tissues were processed for histological and fluorescence analysis. In comparison with noncultured control, a significant reduction was noted in the percentage of normal follicles in all treatments after 1 and 7 days of culture (except treatment with 1 ng/ml of androstenedione for 1 day). As the culture period progressed from 1 to 7 days, treatments with 10 ng/ml of androstenedione + FSH or 50 ng/ml of androstenedione alone maintained the percentage of normal follicles. After 1 day, treatments with 10, 50, or 100 ng/ml of androstenedione + FSH, or with 50 ng/ml of androstenedione alone had more developing follicles than fresh control tissue. When comparing the culture periods, treatments with 1, 10 or 100 ng/ml of androstenedione alone, or FSH alone, or FSH with 1 ng/ml of androstenedione, showed an increase in the percentage of developing follicles. After 1 and 7 days, there were no differences in oocyte and follicular diameter among the treated samples and non-cultured control or MEM+ cultured tissue. Fluorescence analysis demonstrated that only fragments cultured in 50 or 100 ng/ml of androstenedione + FSH had viable preantral follicles similar to those observed in MEM+alone. In conclusion, androstenedione at 50 or 100 ng/ml, either associated with FSH or at 50 ng/ml alone, plays an important role in the maintenance of caprine preantral follicle viability and activation after only a short in vitro culture period. In addition, after 7 days MEM+alone was efficient in the maintenance of viability and in follicular activation, showing the importance of basic medium composition. 650 $aCaprino 650 $aCultura in vitro 650 $aEndocrinologia 650 $aHormônio 650 $aOvário 650 $aReprodução animal 653 $aEsteroidogenese 653 $aFoliculo pre-antral 653 $aFSH 700 1 $aROSSETTO, R. 700 1 $aMATOS, M. H. T. 700 1 $aCELESTINO, J. J. H. 700 1 $aBUENO, J. B. 700 1 $aSILVA, C. M. G. 700 1 $aFAUSTINO, L. R. 700 1 $aMORORÓ, M. B. S. 700 1 $aARAÚJO, V. T. 700 1 $aCAMPELLO, C. C. 700 1 $aFIGUEIREDO, J. R. 773 $tAnimal Reproduction, Belo Horizonte$gv. 7, n. 2, p. 80-89, Apr./Jun. 2010.
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Registros recuperados : 6 | |
1. | | CAVALCANTE, F. A.; MARTINS FILHO, R.; CAMPELLO, C. C.; LOBO, R. N. B.; MARTINS, G. A. Intervalo de partos em rebanho Nelore na Amazônia Oriental. Revista Brasileira de Zootecnia, Piracicaba, v. 29, n. 5, p. 1327-1331, set./out. 2000.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
Biblioteca(s): Embrapa Acre. |
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2. | | CAVALCANTE, F. A.; MARTINS FILHO, R.; CAMPELLO, C. C.; LOBO, R. N. B; MARTINS, G. A. Período de gestação em rebanho nelore na Amazônia Oriental. Revista Brasileira de Zootecnia, Viçosa, MG, v. 30, n. 5, p. 1451-1455, 2001.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - C |
Biblioteca(s): Embrapa Acre. |
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3. | | CAVALCANTE, F. A.; MARTINS FILHO, R.; CAMPELLO, C. C.; LOBO, R. N. B; MARTINS, G. A. Período de serviço em rebanho nelore na Amazônia Oriental. Revista Brasileira de Zootecnia, Viçosa, MG, v. 30, n. 5, p. 1456-1459, 2001.Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - C |
Biblioteca(s): Embrapa Acre. |
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4. | | PAULA, N. R. de O.; ANDRIOLI, A.; CARDOSO, J. de F. S.; PINHEIRO, R. R.; SOUSA, F. M. L.; SOUZA, K. C. de; ALVES, F. S. F.; CAMPELLO, C. C.; RICARTE, A. R. F.; TEIXEIRA, M. F. da S. Profile of the Caprine arthritis-encephalitis virus (CAEV) in blood, semen from bucks naturally and experimentally infected in the Semi-arid region of Brazil. Small Ruminant Research, v. 85, n. 1, p. 27-33, Jul. 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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5. | | ARAÚJO, S. A. C. de; PINHEIRO, R. R.; DANTAS, T. V. M.; ANDRIOLI, A.; LIMA, F. E. S.; DIAS, R. P.; CAMPELLO, C. C.; RICARTE, A. R. F.; MELO, V. S. P. de; ROLIM, B. N.; SILVA, J. B. A. da; TEIXEIRA, M. R. da S. Inibição dos lentivírus de pequenos ruminantes por drogas antivirais. Arquivos do Instituto Biológico, v. 77, n. 2, p. 225-232, abr./jun. 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
Biblioteca(s): Embrapa Caprinos e Ovinos. |
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6. | | PAES, V. M.; VIEIRA, L. A.; CORREIA, H. H. V.; SA, N. A. R.; MOURA, A. A. A.; SALES, A. D.; RODRIGUES, A. P. R.; MAGALHÃES-PADILHA, D. M.; SANTOS, F. W.; APGAR, G. A.; CAMPELLO, C. C.; CAMARGO, L. S. de A.; FIGUEIREDO, J. R. Effect of heat stress on the survival and development of in vitro cultured bovine preantral follicles and on in vitro maturation of cumulus-oocyte complex. Theriogenology, n. 86, p. 994-1003, 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Leite. |
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Registros recuperados : 6 | |
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