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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
01/08/1992 |
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Data da última atualização: |
16/05/2023 |
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Autoria: |
CAMPBELL, R. C.; DOTT, H. M.; GLOVER, T. D. |
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Título: |
Nigrosin eosin as a stain for differentiating live and dead spermatozoa. |
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Ano de publicação: |
1956 |
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Fonte/Imprenta: |
Journal Agricultural Science, v. 48, n. 1, p. 1-8, Oct. 1956. |
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DOI: |
https://doi.org/10.1017/S002185960003029X |
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Idioma: |
Inglês |
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Conteúdo: |
1. The proportion of stained spermatozoa in a ram semen-nigrosin-eosin mixture has been found to increase with the time of exposure to the stain. Five minutes is considered to be a suitable interval for practical use. 2. The value obtained for the proportion of dead cells can be considerably affected by the selection of fields that are easy to count; fields should be chosen at random. 3. The variation between, counts on the same smear is much larger than that expected for binomial proportions. This may be due partly to the "clumping" of the dead spermatozoa and partly to the difficulties of interpretation caused by the presence of many partially stained cells. 4. A significant difference was found between the estimates of the proportion dead obtained by the two experimental workers; this is thought to be due to a difference of interpretation of "stained" and "unstained". 5. Tables are given by which to determine the approximate accuracy of counts made on a range of numbers of spermatozoa, smears and subsamples, separate tables being necessary for ram, boar and bull semen. |
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Palavras-Chave: |
Andrologia; Tecnologia de semen. |
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Thesagro: |
Ovino; Reprodução. |
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Thesaurus Nal: |
Reproduction; Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01784naa a2200241 a 4500
001 1520902
005 2023-05-16
008 1956 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S002185960003029X$2DOI
100 1 $aCAMPBELL, R. C.
245 $aNigrosin eosin as a stain for differentiating live and dead spermatozoa.$h[electronic resource]
260 $c1956
520 $a1. The proportion of stained spermatozoa in a ram semen-nigrosin-eosin mixture has been found to increase with the time of exposure to the stain. Five minutes is considered to be a suitable interval for practical use. 2. The value obtained for the proportion of dead cells can be considerably affected by the selection of fields that are easy to count; fields should be chosen at random. 3. The variation between, counts on the same smear is much larger than that expected for binomial proportions. This may be due partly to the "clumping" of the dead spermatozoa and partly to the difficulties of interpretation caused by the presence of many partially stained cells. 4. A significant difference was found between the estimates of the proportion dead obtained by the two experimental workers; this is thought to be due to a difference of interpretation of "stained" and "unstained". 5. Tables are given by which to determine the approximate accuracy of counts made on a range of numbers of spermatozoa, smears and subsamples, separate tables being necessary for ram, boar and bull semen.
650 $aReproduction
650 $aSheep
650 $aSpermatozoa
650 $aOvino
650 $aReprodução
653 $aAndrologia
653 $aTecnologia de semen
700 1 $aDOTT, H. M.
700 1 $aGLOVER, T. D.
773 $tJournal Agricultural Science$gv. 48, n. 1, p. 1-8, Oct. 1956.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
