Data/hora: 10/06/2024 02:24:51

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 01/08/1992
Data da última atualização: | 16/05/2023
Autoria: | CAMPBELL, R. C.; DOTT, H. M.; GLOVER, T. D.
Título: | Nigrosin eosin as a stain for differentiating live and dead spermatozoa.
Ano de publicação: | 1956
Fonte/Imprenta: | Journal Agricultural Science, v. 48, n. 1, p. 1-8, Oct. 1956.
DOI: | https://doi.org/10.1017/S002185960003029X
Idioma: | Inglês
Conteúdo: | 1. The proportion of stained spermatozoa in a ram semen-nigrosin-eosin mixture has been found to increase with the time of exposure to the stain. Five minutes is considered to be a suitable interval for practical use. 2. The value obtained for the proportion of dead cells can be considerably affected by the selection of fields that are easy to count; fields should be chosen at random. 3. The variation between, counts on the same smear is much larger than that expected for binomial proportions. This may be due partly to the "clumping" of the dead spermatozoa and partly to the difficulties of interpretation caused by the presence of many partially stained cells. 4. A significant difference was found between the estimates of the proportion dead obtained by the two experimental workers; this is thought to be due to a difference of interpretation of "stained" and "unstained". 5. Tables are given by which to determine the approximate accuracy of counts made on a range of numbers of spermatozoa, smears and subsamples, separate tables being necessary for ram, boar and bull semen.
Palavras-Chave: | Andrologia; Tecnologia de semen.
Thesagro: | Ovino; Reprodução.
Thesaurus Nal: | Reproduction; Sheep; Spermatozoa.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (7018-1) - ADD - AP - DD -  -  -  - DIS