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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
04/05/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SCHNEIDER, W. D. H.; COSTA, P. C.; FONTANA, R. C.; SIQUEIRA, F. G. de; DILLON, A. J. P.; CAMASSOLA, M. |
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Afiliação: |
William Daniel Hahn Schneider, Universidade de Caxias do Sul; Paula Cavion Costa, Universidade de Caxias do Sul; Roselei Claudete Fontana, Universidade de Caxias do Sul; FELIX GONCALVES DE SIQUEIRA, CNPAE; Aldo José Pinheiro Dillon, Universidade de Caxias do Sul; Marli Camassola, Universidade de Caxias do Sul. |
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Título: |
Upscale and characterization of lignin-modifying enzymes from Marasmiellus palmivorus VE111 in a bioreactor under parameter optimization and the effect of inducers. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Biotechnology, v. 295, p. 1-8. |
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DOI: |
https://doi.org/10.1016/j.jbiotec.2019.03.002 |
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Idioma: |
Inglês |
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Conteúdo: |
Testing different pHs, dissolved oxygen concentrations and temperatures, plus the addition of inducers, to optimize ligninolytic enzyme activity, resulted in increased production of laccases, total peroxidases and manganese peroxidases on the order of 2.1-fold, 4.6-fold and 10-fold, respectively; laccases reached 6588 U/mL, total peroxidases reached 3533 U/mL and manganese peroxidase achieved 60 U/mL. Furthermore, an increase in laccase volumetric productivity and in its specific activity was verified. The addition of inducers, such as copper sulphate and manganese sulphate, improved enzymatic activity. In addition, a new previously unidentified laccase isoform was documented by zymography. The present work successfully increased the production of ligninolytic enzymes. |
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Palavras-Chave: |
Copper sulphate; Laccases; White-rot basidiomycete. |
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Thesaurus Nal: |
Lignin; Peroxidases. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01628naa a2200253 a 4500
001 2108693
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.jbiotec.2019.03.002$2DOI
100 1 $aSCHNEIDER, W. D. H.
245 $aUpscale and characterization of lignin-modifying enzymes from Marasmiellus palmivorus VE111 in a bioreactor under parameter optimization and the effect of inducers.$h[electronic resource]
260 $c2019
520 $aTesting different pHs, dissolved oxygen concentrations and temperatures, plus the addition of inducers, to optimize ligninolytic enzyme activity, resulted in increased production of laccases, total peroxidases and manganese peroxidases on the order of 2.1-fold, 4.6-fold and 10-fold, respectively; laccases reached 6588 U/mL, total peroxidases reached 3533 U/mL and manganese peroxidase achieved 60 U/mL. Furthermore, an increase in laccase volumetric productivity and in its specific activity was verified. The addition of inducers, such as copper sulphate and manganese sulphate, improved enzymatic activity. In addition, a new previously unidentified laccase isoform was documented by zymography. The present work successfully increased the production of ligninolytic enzymes.
650 $aLignin
650 $aPeroxidases
653 $aCopper sulphate
653 $aLaccases
653 $aWhite-rot basidiomycete
700 1 $aCOSTA, P. C.
700 1 $aFONTANA, R. C.
700 1 $aSIQUEIRA, F. G. de
700 1 $aDILLON, A. J. P.
700 1 $aCAMASSOLA, M.
773 $tJournal of Biotechnology$gv. 295, p. 1-8.
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Embrapa Agroenergia (CNPAE) |
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| 20. |  | SIMÃO, B. R.; BRITO, L. O.; MAIA, A. S. C.; MIRANDA, L. C.; AZEVEDO, C. M. da S. B. Stocking densities and feeding stategies in shrimp and tilapia polyculture in tanks. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 8, p. 1088-1095, ago. 2013. Título em português: Densidades de estocagem e estratégias de alimentação em policultivo de camarão e tilápia em tanques.| Biblioteca(s): Embrapa Unidades Centrais. |
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