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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
08/06/2018 |
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Data da última atualização: |
29/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOSCO, D. D.; SINSKI, I.; RITSCHEL, P. S.; CAMARGO, U. A.; FAJARDO, T. V. M.; HARAKAVA, R.; QUECINI, V. |
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Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; Umberto A. Camargo; THOR VINICIUS MARTINS FAJARDO, CNPUV; Ricardo Harakava; VERA MARIA QUECINI, CNPUV. |
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Título: |
Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Transgenic Research, p. 1-18, 2018. [Online] |
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DOI: |
https://doi.org/10.1007/s11248-018-0082-1 |
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Idioma: |
Português |
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Conteúdo: |
Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis MenosIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and... Mostrar Tudo |
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Palavras-Chave: |
Fungus; Pathogenesis related protein 5. |
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Thesaurus Nal: |
Chitinase; Epigenetics; Grapevine leafroll-associated virus 3; Vitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178409/1/s11248-018-0082-1-Artigo-v-publicada-Metilacao-DNA-Transgenic-Research-06-06-18.pdf
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Marc: |
LEADER 02824naa a2200277 a 4500
001 2092380
005 2019-04-29
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11248-018-0082-1$2DOI
100 1 $aBOSCO, D. D.
245 $aExpression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.$h[electronic resource]
260 $c2018
520 $aIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis
650 $aChitinase
650 $aEpigenetics
650 $aGrapevine leafroll-associated virus 3
650 $aVitis
653 $aFungus
653 $aPathogenesis related protein 5
700 1 $aSINSKI, I.
700 1 $aRITSCHEL, P. S.
700 1 $aCAMARGO, U. A.
700 1 $aFAJARDO, T. V. M.
700 1 $aHARAKAVA, R.
700 1 $aQUECINI, V.
773 $tTransgenic Research, p. 1-18, 2018. [Online]
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Biblioteca |
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Registro |
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| Registros recuperados : 181 | |
| 161. |  | CAMARGO, U. A.; MAIA, J. D. G.; REVERS, L. F.; LEAO, P. C. de S.; QUECINI, V.; FERREIRA, M. E.; RITSCHEL, P. S. Grape breeding and genetic studies in Brazil. In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...] [S.l.]: International Crop Science Society, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 162. | | CAMARGO, U. A.; MAIA, J. D. G.; REVERS, L. F.; LEAO, P. C. de S.; QUECINI, V.; FERREIRA, M. E.; RITSCHEL, P. S. Grape genetics and breeding in Brazil. Journal of Basic and Applied Genetics, Buenos Aires, v. 23, p. 23, 2012. Suplemento. Resumo apresentado no XV Congreso Latinoamericano de Genética, 2012, Rosário, Argentina.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 163. | | RITSCHEL, P. S.; MAIA, J. D. G.; CAMARGO, U. A.; ZANUS, M. C.; TAFFAREL, J. C.; GROHS, D. S.; FAJARDO, T. V. M. BRS Bibiana: cultivar de uva tolerante à podridão cinzenta e à podridão ácida, para elaboração de vinho branco aromático, recomendada para a Serra Gaúcha. Bento Gonçalves, RS: Embrapa Uva e Vinho, 2019. (Embrapa Uva e Vinho. Circular Técnica, 147)| Biblioteca(s): Embrapa Uva e Vinho. |
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| 165. | | RITSCHEL, P. S.; MAIA, J. D. G.; CAMARGO, U. A.; SOUZA, R. T. de; FAJARDO, T. V. M.; NAVES, R. de L.; GIRARDI, C. L. BRS Isis: nova cultivar de uva de mesa vermelha, sem sementes e tolerante ao míldio. Bento Gonçalves: Embrapa Uva e Vinho, 2013. 20 p. il., color. (Embrapa Uva e Vinho. Comunicado Técnico, 143).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 166. | | RITSCHEL, P. S.; MAIA, J. D. G.; CAMARGO, U. A.; SOUZA, R. T. de; FAJARDO, T. V. M.; NAVES, R. de L.; GIRARDI, C. L. 'BRS Isis': a novel red seedless table grape cultivar tolerance to downy mildew. In:CONGRESSO LATINO-AMERICANO DE VITICULTURA E ENOLOGIA, 15., 2015, Bento Gonçalves, RS. p 380.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 169. | | RITSCHEL, P. S.; MAIA, J. D. G.; CAMARGO, U. A.; ZANUS, M. C.; SOUZA, R. T. de; FAJARDO, T. V. M. 'BRS Magna': a novel grape cultivar for juice making, with wide climatic adaptation. Crop Breeding and Applied Biotechnology, v. 14, n. 4, p. 266-269, 2014. Cultivar release.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 170. | | RITSCHEL, P. S.; MAIA, J. D. G.; CAMARGO, U. A.; ZANUS, M. C.; SOUZA, R. T. de; FAJARDO, T. V. M. 'BRS Magna': a novel grape cultivar for juice making, with wide climatic adaptation. In:CONGRESSO LATINO-AMERICANO DE VITICULTURA E ENOLOGIA, 15., 2015, Bento Gonçalves, RS. p 381.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 171. | | MAIA, J. D. G.; RITSCHEL, P. S.; CAMARGO, U. A.; SOUZA, R. T. de; GROHS, D. S.; FAJARDO, T. V. M. BRS Melodia: nova cultivar de uvas sem sementes, com sabor especial de mix de frutas vermelhas, recomendada para cultivo na Serra Gaúcha, em cobertura plástica. Bento Gonçalves, RS: Embrapa Uva e vinho, 2019. (Embrapa Uva e Vinho. Circular Técnica, 144)| Biblioteca(s): Embrapa Uva e Vinho. |
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| 173. | | MAIA, J. D. G.; RITSCHEL, P. S.; CAMARGO, U. A.; SOUZA, R. T. de; FAJARDO, T. V. M.; NAVES, R. de L.; GIRARDI, C. L. 'BRS Vitória': nova cultivar de uva de mesa sem sementes com sabor especial e tolerante ao míldio. Bento Gonçalves: Embrapa Uva e Vinho, 2012. 12 p. il., color. (Embrapa Uva e Vinho. Comunicado Técnico, 126).| Tipo: Comunicado Técnico/Recomendações Técnicas |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 174. | | MAIA, J. D. G.; RITSCHEL, P. S.; CAMARGO, U. A.; SOUZA, R. T. de; FAJARDO, T. V. M.; NAVES, R. de L.; GIRARDI, C. L. 'BRS Vitória': a novel seedless table grape cultivar exhibiting special flavor and tolerance to downy mildew (Pasmopara viticola). Crop Breeding and Applied Biotechnology, v. 14, n. 3, p. 204-206, 2014. Cultivar release.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 175. | | RITSCHEL, P. S.; GIRARDI, C. L.; ZANUS, M. C.; FAJARDO, T. V. M.; MAIA, J. D. G.; SOUZA, R. T. de; NAVES, R. de L.; CAMARGO, U. A. Novel Brazilian grape cultivars. Yanqing, Beijing, China: ISHS Acta Horticulturae 1082, April 2015. Proceedings of the Eleventh International Conference on Grapevine Breeding and Genetics. p. 157-163.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 176. | | SILVA, G. A. da; BRANDOLT, S. D. F.; POLI, J. S.; POLETTO, C. M.; MELLO, L. M. R. de; ZANUS, M. C.; CAMARGO, U. A. Polyphenols and antioxidant content in grape juice. In: CONGRESSO MONDIALE DELLA VIGNA E DEL VINO, 31.; ASSEMBLEA GENERALE DELL'O.I.V, 6., Verona, 2008. [Proceedings...] [S.l.]: OIV: Ministereo Delle Politiche Agricole, Alimentare e Forestali, [2008]. Não paginado. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 177. | | SILVA, G. A. da; BRANDOLT, S. D. F.; POLI, J. S.; POLETTO, C. M.; MELLO, L. M. R. de; ZANUS, M. C.; CAMARGO, U. A. Polyphenols and antioxidant content in grape juice. In: CONGRESSO MONDIALE DELLA VIGNA E DEL VINO, 31.; ASSEMBLEA GENERALE DELL'O.I.V., 6., 2008, Verona, Italia. Riassunti delle comunicazioni. [Verona]: OIV: Ministero Politiche Agricole, Alimentari e Forestali, 2008. p. 230. Resumo P.I.80.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 178. | | POLI, J. S.; SILVA, G. A. da; CAMARGO, U. A.; ZANUS, M. C.; BONA, G. DE; GURAK, P. D.; MORINI, M. A. L. Teores de polifenóis, antocianas, capacidade antioxidante e intensidade de cor em diferentes cultivares e seleções de uva para suco. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 4., 2006, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2006. p. 23| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Uva e Vinho. |
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| 180. | | MATSUURA, F. C. A. U.; BOLSON, E. A.; FOLEGATTI, M. I. da S.; CAMARGO, U. A.; GUTIEREZ, A. de S. D.; SPOTO, M. H. F.; RODRIGUES, V. M.; PECCINI, J. R. Nova variedade de uva sem semente nacional - 'seleção 8': avaliação da qualidade junto aos canais de distribuição. CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 20., 2006, Curitiba. Alimentos e agroindústrias brasileiras no contexto internacional: anais. Curitiba: Sociedade Brasileira de Ciência e Tecnologia de Alimentos, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Unidades Centrais; Embrapa Uva e Vinho. |
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| Registros recuperados : 181 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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