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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. |
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Afiliação: |
JEFERSON FERREIRA DA FONSECA, CNPC; Ribrio Ivan Tavares Pereira Batista; Juliane Teramachi Trevizan; Joanna Maria Gonçalves Souza-Fabjan; Felipe Zandonadi Brandão; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019. |
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DOI: |
10.5433/1679-0359.2019v40n6Supl3p3789 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation. MenosAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that trans... Mostrar Tudo |
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Palavras-Chave: |
Bovine oocytes; COCs; Ex-situ maturation; In vitro maturation; Nonsurgical embryo transfer technique. |
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Thesaurus Nal: |
Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/208736/1/Goat-incubator-can-bovine-oocytes.pdf
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Marc: |
LEADER 02452naa a2200265 a 4500
001 2118709
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $a10.5433/1679-0359.2019v40n6Supl3p3789$2DOI
100 1 $aFONSECA, J. F. da
245 $aGoat incubator$bcan bovine oocytes be matured in the uterine horn of a goat?$h[electronic resource]
260 $c2019
520 $aAbstract We used a goat as a live incubator, along with associated nonsurgical embryo transfer techniques, to perform ex situ (in vivo) maturation of bovine oocytes. Immature bovine cumulus-oocyte complexes (COCs) aspirated from 3-8 mm follicles from slaughterhouse ovaries were randomly split into two groups for in vitro (IVM; n = 38) and ex situ maturation (ESM; n = 40). IVM was performed for a period of 24 h at 38.5 ºC and with 5% CO2 in the air of maximum humidity. For ESM, a presynchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apiece, via the transcervical route. After 24 h the structures were retrieved through uterine flushing. Analyses of nuclear maturation and lipid quantification were performed on oocytes from both groups. Fluorescent intensity was compared using the Student?s t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for the ESM and IVM groups, respectively. In vitro-matured COCs contained more lipid droplets, expressed as a higher amount (p < 0.05) of emitted fluorescent light than ex situ-matured COCs (858 ± 73 vs. 550 ± 64 arbitrary fluorescence units, respectively). This is the first report to associate nonsurgical embryo transfer techniques and a goat as a live incubator for the maturation of bovine oocytes. We conclude that bovine oocytes can progress meiotically in the uterus horn of a goat and that transcervical transfer of bovine oocytes to a goat?s uterus could present an alternative to nuclear maturation.
650 $aGoats
653 $aBovine oocytes
653 $aCOCs
653 $aEx-situ maturation
653 $aIn vitro maturation
653 $aNonsurgical embryo transfer technique
700 1 $aBATISTA, R. I. T. P.
700 1 $aTREVIZAN, J. T.
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aBRANDÃO, F. Z.
700 1 $aCAMARGO, L. S. de A.
773 $tSemina: Ciências Agrárias, Londrina$gv. 40, n. 6, supp. 3, p. 3789-3796, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 7 | |
| 3. |  | OLMOS, F.; SOSA, M.; CARMO, M. do; CAL, V.; BETANCUR, D.; SOCA, P.; GARCÍA, E.; GENRO, C. Monitoreo de cambios en la composición botánica. In: SOCA, P.; ESPASANDÍN, A.; CARRIQUIRY, M. Efecto de la oferta de forraje y grupo genético de las vacas sobre la productividad y sostenibilidad de la cría vacuna en campo natural. Montevideo: INIA, 2013. p. 73-78. (INIA. FPTA, 48).| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 4. | | OLMOS, F.; SOCA, P.; SOSA, M.; CARMO, M. Do; CAL, V.; BENTANCUR, D.; GENRO, T. C. M.; GARCÍA, E. Respuesta a manejo de factores ecológicos en Coelorachis selloana (Hack) en la región noroeste de Uruguay. In: CONGRESO NACIONAL, 6.; CONGRESO DEL MERCOSUR, 3.; JORNADA TÉCNICA DE PRODUCTORES, 2., 2013, Santa Rosa, Argentina. Los pastizales y el hombre, producir y conservar: actas. Santa Rosa: Universidad Nacional de la Pampa, 2013. p. 121. CONGRESO ARGENTINO DE PASTIZALES.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 5. | | PURSLOW, P. P.; CEPEDA, R.; CAFFERO, E.; GARITTA, L. V.; SOSA, M.; FRANK, D.; DUIZER, L.; NGAPO, T.; BRUCE, H.; NASSU, R. T.; HENCHION, M. Differences in consumer perceptions of meat quality. In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY, 63., 2017, Cork, Ireland. Proceedings... Cork, Ireland: Wageningen Academic Publishers, 2017. p. 766-767.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. | | PURSLOW, P.; CEPEDA, R.; CÁFFARO, E.; GARITTA, L.; SOSA, M.; FRANK, D.; DUIZER, L.; NGAPO, T.; BRUCE, H.; NASSU, R. T.; HINCHION, M. Differences in the responses of ICoMST delegates to a meat consumer survey compared to mon-expert consumers in five countries: hygiene and food safety aspects. In: INTERNATIONAL CONGRESS OF MEAT SCIENCE AND TECHNOLOGY, 64., 2018, Melbourne, Australia. Proceedings... Melbourne, Australia: ICOMST, 2018.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 7. | | NARDO, E. A. B. de; PASSALACQUA, S. A.; DIAS, J. M. C. S.; MONDACA RIVAS, P. S.; ARES, M. I.; ARCE BUSTAMANTE, R.; CHIARAVALLE, W.; SOSA, M. N. B. Comitê de sanidade vegetal do Cone Sul (COSAVE) aprova regulamentação para registro experimental e comercial de agentes microbianos de controle de pragas. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 6., 1998, Rio de Janeiro. Anais: sessões de pôsteres. Rio de Janeiro: FIOCRUZ/EMBRAPA, 1998. p.206.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 7 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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