Data/hora: 23/09/2024 23:07:48

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	14/01/2020	
Data da última atualização: 	06/02/2024	
Autoria: 	OLIVEIRA, C. S.; SERAPIÃO, R. V.; CAMARGO, A. J. DOS R.; FREITAS, C. de; IGUMA, L. T.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; OLIVEIRA, L. Z.; VERNEQUE, R. da S.	
Afiliação: 	CLARA SLADE OLIVEIRA, CNPGL; Raquel Varella Serapião; Agostinho Jorge dos Reis Camargo; CELIO DE FREITAS, CNPGL; Lilian Tamy Iguma; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Letícia Zoccolaro Oliveira; RUI DA SILVA VERNEQUE, CNPGL.	
Título: 	Oocyte origin affects the in vitro embryo production and development of Holstein (Bos taurus taurus) - Gyr (Bos taurus indicus) reciprocal cross embryos.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	Animal Reproduction Science, v. 209, article 106165, 2019.	
DOI: 	https://doi.org/10.1016/j.anireprosci.2019.106165	
Idioma: 	Inglês	
Conteúdo: 	A reciprocal crossbred embryo production approach was used to assess effects of maternal breed on embryo development in tropical conditions (average temperature 22.0 °C and 77.9% relative humidity). Oocytes were recovered by ovum pick-up (OPU) from Gyr and Holstein donors (n = 90 Holstein and 83 Gyr OPUs). Female F1 embryos were produced by fertilization with sperm bearing X-chromosomes from Holstein semen (n = 615 Gyr oocytes) or Gyr semen (n = 255 Holstein oocytes). Blastocysts were transferred to recipients 168 h post-insemination (h.p.i.) (n= 70?144) and there were assessments of pregnancies until birth. Oocyte number per OPU (Gyr 10.0 ± 0.7 compared with Holstein 6.3 ± 0.4) and percentage viable oocytes (Gyr 78.8 ± 1.9% compared with Holstein 71.2 ± 2.2%) were less for Holstein donor animals. There was a 2.8 fold fewer total number of F1 blastocysts when Holstein donors were used (Gyr: 260, Holstein: 91). Pregnancy assessment during the different stages of gestation indicated the percentage pregnancy was less when embryos were produced from Holstein oocytes (Gyr and Holstein respectively: early pregnancy, 47.9% compared with 38.6%; mid-pregnancy, 44.4% compared with 31.4%; late pregnancy, 41.0% compared with 22.9%). Pregnancy length was also affected by maternal breed (Gyr: 280.8 ± 0.6, Holstein: 286.3 ± 0.7). It is concluded that in a tropical environment the maternal breed affects crossbred embryo development with pregnancy rates during the latter stages of gestation being greater when Gyr oocytes are used for production of embryos.	
Palavras-Chave: 	Maternal breed; Oocyte quality.	
Thesaurus Nal: 	Crossbreds; Gir (cattle breed); Girolando.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (24974-1) - UPC - AP - DD -  -  -  - DIS