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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OTERO, R.; HERNÁNDEZ, D.; CAMARGO, L. S. de A. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Production of bovine transgenic embryos by microinjection of a lentiviral vector in mature ovocytes. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Indian Journal of Science and Technology, v. 11, n. 31, 2018. |
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Páginas: |
8 p. |
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DOI: |
10.17485/ijst/2018/v11i31/130839 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Objective: To produce bovine transgenic embryos by microinjection of a lentiviral vector with the eGFP gene as a marker. Methods: Four treatments were designed: T1=Control: fertilized in vitro (FIV) with cumulus-oocyte complexes (CCOs), cultivated in CR2 medium with 10% FBS and incubated at 38.5°C in an atmosphere of 95% humidity and 5% CO₂. T2=Control of culture medium: CCOs removed by vortex in the presence of hyaluronidase, FIV, grown in SOF medium in hermetic bag, with a gaseous mixture of 5% CO₂, 5% O₂ and 90% N₂ and humidity saturated at 38.5°C. T3=Microinjection control: CCOs removed microinjected with TALP medium, FIV and cultured under the same treatment conditions T2. T4=Microinjected with the lentivirus: CCOs removed microinjected with the lentiviral vector and FIV and cultured in the same conditions of the T2 and T3 treatments. The rate of development of blastocysts at day eight and the expression of the eGFP gene were evaluated. Findings: No significant statistical differences were found (p> 0.05) in the production of blastocysts at day eight, between treatments T1, T2, and T3. The percentage of blastocysts found in the T4 treatment was significantly lower (p <0.05) than in the other treatments. All embryos obtained in T4 expressed the transgene of interest. Application / Improvements: It is concluded that the culture conditions used were adequate for T1, T2 and T3, added that the microinjection with the lentiviral vector influences in some way the embryonic development, although, the technique was highly efficient for obtaining transgenic embryos. MenosAbstract Objective: To produce bovine transgenic embryos by microinjection of a lentiviral vector with the eGFP gene as a marker. Methods: Four treatments were designed: T1=Control: fertilized in vitro (FIV) with cumulus-oocyte complexes (CCOs), cultivated in CR2 medium with 10% FBS and incubated at 38.5°C in an atmosphere of 95% humidity and 5% CO₂. T2=Control of culture medium: CCOs removed by vortex in the presence of hyaluronidase, FIV, grown in SOF medium in hermetic bag, with a gaseous mixture of 5% CO₂, 5% O₂ and 90% N₂ and humidity saturated at 38.5°C. T3=Microinjection control: CCOs removed microinjected with TALP medium, FIV and cultured under the same treatment conditions T2. T4=Microinjected with the lentivirus: CCOs removed microinjected with the lentiviral vector and FIV and cultured in the same conditions of the T2 and T3 treatments. The rate of development of blastocysts at day eight and the expression of the eGFP gene were evaluated. Findings: No significant statistical differences were found (p> 0.05) in the production of blastocysts at day eight, between treatments T1, T2, and T3. The percentage of blastocysts found in the T4 treatment was significantly lower (p <0.05) than in the other treatments. All embryos obtained in T4 expressed the transgene of interest. Application / Improvements: It is concluded that the culture conditions used were adequate for T1, T2 and T3, added that the microinjection with the lentiviral vector influen... Mostrar Tudo |
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Palavras-Chave: |
EGFP; Genetic Modification; Micromanipulation. |
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Thesaurus Nal: |
Green fluorescent protein. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189326/1/Artigo-Indian-J-Sci-and-Tech-Camargo-Production.pdf
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Marc: |
LEADER 02307naa a2200217 a 4500
001 2102529
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.17485/ijst/2018/v11i31/130839$2DOI
100 1 $aOTERO, R.
245 $aProduction of bovine transgenic embryos by microinjection of a lentiviral vector in mature ovocytes.$h[electronic resource]
260 $c2018
300 $a8 p.
520 $aAbstract Objective: To produce bovine transgenic embryos by microinjection of a lentiviral vector with the eGFP gene as a marker. Methods: Four treatments were designed: T1=Control: fertilized in vitro (FIV) with cumulus-oocyte complexes (CCOs), cultivated in CR2 medium with 10% FBS and incubated at 38.5°C in an atmosphere of 95% humidity and 5% CO₂. T2=Control of culture medium: CCOs removed by vortex in the presence of hyaluronidase, FIV, grown in SOF medium in hermetic bag, with a gaseous mixture of 5% CO₂, 5% O₂ and 90% N₂ and humidity saturated at 38.5°C. T3=Microinjection control: CCOs removed microinjected with TALP medium, FIV and cultured under the same treatment conditions T2. T4=Microinjected with the lentivirus: CCOs removed microinjected with the lentiviral vector and FIV and cultured in the same conditions of the T2 and T3 treatments. The rate of development of blastocysts at day eight and the expression of the eGFP gene were evaluated. Findings: No significant statistical differences were found (p> 0.05) in the production of blastocysts at day eight, between treatments T1, T2, and T3. The percentage of blastocysts found in the T4 treatment was significantly lower (p <0.05) than in the other treatments. All embryos obtained in T4 expressed the transgene of interest. Application / Improvements: It is concluded that the culture conditions used were adequate for T1, T2 and T3, added that the microinjection with the lentiviral vector influences in some way the embryonic development, although, the technique was highly efficient for obtaining transgenic embryos.
650 $aGreen fluorescent protein
653 $aEGFP
653 $aGenetic Modification
653 $aMicromanipulation
700 1 $aHERNÁNDEZ, D.
700 1 $aCAMARGO, L. S. de A.
773 $tIndian Journal of Science and Technology$gv. 11, n. 31, 2018.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 395 | |
| 14. |  | IGUMA, L. T.; SÁ, W. F. de; VIANA, J. H. M.; CAMARGO, L. S. de A. Avances tecnólogicos en la reproducción animal: inseminación artificial. In: MARTINS, P. do C.; DINIZ, F. H.; MOREIRA, M. S. de P.; NOGUEIRA NETTO, V.; ARCURI, P. B. (Ed.). Conocimientos y estratégias tecnológicas para la producción de leche en regiones tropicales. Juiz de Fora: Embrapa Gado de Leite, 2007. p. 309-319.| Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | VIANA, J. H. M.; IGUMA, L. T.; CAMARGO, L. S. de A.; SÁ, W. F. de. Avances tecnológicos en la reproducción animal: superovulación y transferencia de embriones. In: MARTINS, P. do C.; DINIZ, F. H.; MOREIRA, M. S. de P.; NOGUEIRA NETTO, V.; ARCURI, P. B. (Ed.). Conocimientos y estratégias tecnológicas para la producción de leche en regiones tropicales. Juiz de Fora: Embrapa Gado de Leite, 2007. p. 321-332.| Tipo: Capítulo em Livro Técnico-Científico | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | CAMARGO, L. S. de A.; OLIVEIRA, C. S.; PFEIFER, L. F. M.; MACHADO, R. Avanços e inovações em biotécnicas da reprodução em bovinos de leite. In: VILELA, D.; FERREIRA, R. de P.; FERNANDES, E. N.; JUNTOLLI, F. V. (Ed.). Pecuária de leite no Brasil: cenários e avanços tecnológicos. Brasília, DF: Embrapa, 2016. p. 325-343.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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| Registros recuperados : 395 | |
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