Data/hora: 23/09/2024 18:06:32

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	13/08/2021	
Data da última atualização: 	13/08/2021	
Autoria: 	OLIVEIRA, C. S.; FEUCHARD, V. L. da S.; FREITAS, C. de; ROSA, P. M. da S.; CAMARGO, A. J. dos R.; SARAIVA, N. Z.	
Afiliação: 	CLARA SLADE OLIVEIRA, CNPGL; VIVIANE LUZIA DA SILVA FEUCHARD, Pesagro; CELIO DE FREITAS, CNPGL; PAOLA MARIA DA SILVA ROSA; AGOSTINHO JORGE DOS REIS CAMARGO, Pesagro; NAIARA ZOCCAL SARAIVA, CNPGL.	
Título: 	In-straw warming protocol improves survival of vitrified embryos and allows direct transfer in cattle.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Cryobiology, v. 97, p. 222-225, 2020.	
DOI: 	https://doi.org/10.1016/j.cryobiol.2020.02.007	
Idioma: 	Inglês	
Conteúdo: 	Vitrification is a superior method for cryopreservation of IVF embryos, but due to complicated warming protocols, it is not commonly used for commercial bovine embryos routine. To overcome the need of laboratory embryo preparation during warming, we developed an in-straw warming protocol compatible with most vitrification devices for embryo transfer without sucrose gradient steps and embryo evaluation. Surprisingly, one of the tested protocols improved embryo survival (95.0%* vs 83.1% expansion rate and 74.2%* vs 51.5% hatching rate) compared to conventional in-plate warming. Embryo quality was also increased, taken by the higher total cell numbers (160.7 ± 8.6* vs 99.0 ± 7.9) and lower apoptosis index (4.9 ±0.6* vs 11.5 ± 2.4) 48 h after warming. Pregnancy rates were similar between vitrified-warmed embryos and fresh embryos (40% vs 43%). Based on our results, we suggest in-straw warming should always be used for vitrified embryos due to beneficial effects. Direct transfer can be safely performed using this protocol.	
Palavras-Chave: 	Criotolerância; Fertilização in vitro; Vitrificação.	
Thesagro: 	Bovino; Criopreservação; Embrião Animal; Palha; Reprodução Animal; Sacarose.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (25207-1) - UPC - AP - DD -  -  -  - DIS