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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
17/09/1997 |
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Data da última atualização: |
17/09/1997 |
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Autoria: |
BECKER, J. M.; CALDWELL, G. A.; ZACHGO, E. A. |
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Título: |
Biotechnology: a laboratory course. |
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Edição: |
2. ed. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
San Diego: Academic Press, 1996. |
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Páginas: |
261p. |
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ISBN: |
0-12-084562-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Asseptic technique and establishing pure cultures: the streak plate and culture transfer. Preparation of culture media. The growth curve. Isolation of plasmid DNA from escherichia coli: the mini-prep. Purification, concentration, and quantitation of DNA. Large-scale isolation of plasmid DNA by column chromatography. Amplification of a lacZ Gene fragment by the polymerase chain reaction. Restriction digestion and agarose gel electrophoresis. Southern transfer. Preparation, purification, and hybridization of probe. Transformation of saccharomyces cerevissiae. Isolation of plasmid from yeast and escherichia coli transformation. Protein assays. Qualitative assay for B-galactosidase in yeast colonies. Determination of B-galactosidase in permeabilized yeast cells. Assay of B-galactosidase in cell extracts. B-galactosidase purification. Western blot: probe of protein blot with antibody to B-galactosidase. Alternative protocols and experiments. Buffer solutions. Preparation of buffers and solutions. Properties of some common concentrated acids and bases. Use of micropipettors. List of cultures. Storage of cultures and DNA. Sterilization methods. Preparation of stock solutions for culture media. Growth in liquid medium. Determination of viable cells. Determination of cell mass. Determination of cell number. Nomenclature of strains. Glassware and plasticware. Preparation of tris and EDTA. Basic rules for handling enzymes. Effects of common contaminants on protein assays. Manufacturers' and distributors' addresses.... MenosAsseptic technique and establishing pure cultures: the streak plate and culture transfer. Preparation of culture media. The growth curve. Isolation of plasmid DNA from escherichia coli: the mini-prep. Purification, concentration, and quantitation of DNA. Large-scale isolation of plasmid DNA by column chromatography. Amplification of a lacZ Gene fragment by the polymerase chain reaction. Restriction digestion and agarose gel electrophoresis. Southern transfer. Preparation, purification, and hybridization of probe. Transformation of saccharomyces cerevissiae. Isolation of plasmid from yeast and escherichia coli transformation. Protein assays. Qualitative assay for B-galactosidase in yeast colonies. Determination of B-galactosidase in permeabilized yeast cells. Assay of B-galactosidase in cell extracts. B-galactosidase purification. Western blot: probe of protein blot with antibody to B-galactosidase. Alternative protocols and experiments. Buffer solutions. Preparation of buffers and solutions. Properties of some common concentrated acids and bases. Use of micropipettors. List of cultures. Storage of cultures and DNA. Sterilization methods. Preparation of stock solutions for culture media. Growth in liquid medium. Determination of viable cells. Determination of cell mass. Determination of cell number. Nomenclature of strains. Glassware and plasticware. Preparation of tris and EDTA. Basic rules for handling enzymes. Effects of common contaminants on protein assays. Manufacturers... Mostrar Tudo |
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Palavras-Chave: |
Biotecnology. |
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Thesagro: |
Biotecnologia; Laboratório. |
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Thesaurus Nal: |
laboratories. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02055nam a2200217 a 4500
001 1557086
005 1997-09-17
008 1996 bl uuuu 00u1 u #d
020 $a0-12-084562-8
100 1 $aBECKER, J. M.
245 $aBiotechnology$ba laboratory course.
250 $a2. ed.
260 $aSan Diego: Academic Press$c1996
300 $a261p.
520 $aAsseptic technique and establishing pure cultures: the streak plate and culture transfer. Preparation of culture media. The growth curve. Isolation of plasmid DNA from escherichia coli: the mini-prep. Purification, concentration, and quantitation of DNA. Large-scale isolation of plasmid DNA by column chromatography. Amplification of a lacZ Gene fragment by the polymerase chain reaction. Restriction digestion and agarose gel electrophoresis. Southern transfer. Preparation, purification, and hybridization of probe. Transformation of saccharomyces cerevissiae. Isolation of plasmid from yeast and escherichia coli transformation. Protein assays. Qualitative assay for B-galactosidase in yeast colonies. Determination of B-galactosidase in permeabilized yeast cells. Assay of B-galactosidase in cell extracts. B-galactosidase purification. Western blot: probe of protein blot with antibody to B-galactosidase. Alternative protocols and experiments. Buffer solutions. Preparation of buffers and solutions. Properties of some common concentrated acids and bases. Use of micropipettors. List of cultures. Storage of cultures and DNA. Sterilization methods. Preparation of stock solutions for culture media. Growth in liquid medium. Determination of viable cells. Determination of cell mass. Determination of cell number. Nomenclature of strains. Glassware and plasticware. Preparation of tris and EDTA. Basic rules for handling enzymes. Effects of common contaminants on protein assays. Manufacturers' and distributors' addresses....
650 $alaboratories
650 $aBiotecnologia
650 $aLaboratório
653 $aBiotecnology
700 1 $aCALDWELL, G. A.
700 1 $aZACHGO, E. A.
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Embrapa Cerrados (CPAC) |
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