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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
02/03/2016 |
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Data da última atualização: |
09/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, F. C.; CLEMENTE, A. da C. S.; CAIXETA, F.; ROSA, S. D. V. F. da. |
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Afiliação: |
UDI Pesquisa & Desenvolvimento; UFLA; General Mills Brasil Alimentos Ltda.; STTELA DELLYZETE VEIGA F DA ROSA, SAPC. |
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Título: |
Endo-ß-mannanase and ß-tubulin gene expression during the final phases of coffee seed maturation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 4, p. 11719-11728, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee seed maturation. MenosCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee se... Mostrar Tudo |
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Palavras-Chave: |
QRT-PCR. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Developmental stages. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140475/1/Endo-B-mannanase.pdf
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Marc: |
LEADER 02122naa a2200193 a 4500
001 2039275
005 2019-05-09
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C.
245 $aEndo-ß-mannanase and ß-tubulin gene expression during the final phases of coffee seed maturation.$h[electronic resource]
260 $c2015
520 $aCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee seed maturation.
650 $aDevelopmental stages
650 $aCoffea Arábica
653 $aQRT-PCR
700 1 $aCLEMENTE, A. da C. S.
700 1 $aCAIXETA, F.
700 1 $aROSA, S. D. V. F. da
773 $tGenetics and Molecular Research$gv. 14, n. 4, p. 11719-11728, 2015.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 34 | |
| 5. |  | CLEMENTE, A. da C. S.; CIRILLO, M. A.; MALTA, M. R.; CAIXETA, F.; ROSA, S. D. V. F. da. Aplicações de técnicas de análise multivariada na avaliação da qualidade química e sensorial de café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 6. | | ROSA, S. D. V. F. da; CAIXETA, F.; CLEMENTE, A. da C. S.; PEREIRA, C. C.; SANTOS, F. C. Aspectos fisiológicos de grãos de café armazenados em ambiente resfriado. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 7. | | FARIA, O. A. C.; CAIXETA, F. M.; DIAS, L. R. O.; SPRÍCIGO, J. F. W.; DODE, M. A. N. Effect of the anticipation of intrafollicular transfer of immature oocytes (IFIOT) in the nuclear maturation of bovine oocyte. Animal Reproduction, v. 16, n. 3, p. 587, Jul./Sept. 2019. Edição dos Proceedings of the 33nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Ilha da Comandatuba, BA, Brazil, August 15th to 19th, 2019.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | RODRIGUES, S. de A. D.; PONTELO, T. P.; KAWAMOTO, T. S.; CAIXETA, F. M. C.; DODE, M. A. N. Study on nuclear maturation kinetics of bovine ovocyts with different degrees of competence. Animal Reprodroduction, v. 14, n. 3, p. 798, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | REIS, R. de G. E.; CLEMENTE, A. da C. S.; CAIXETA, F.; PEREIRA, C. C.; COELHO, L. F. S.; ROSA, S. D. V. F. da; SILVA, A. A. Atividade antioxidante de grãos de café submetidos a diferentes processamentos e armazenados. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 13. | | CATÃO, H. C. R. M.; GOMES, L. A. A.; SANTOS, H. O. dos; GUIMARÃES, R. M.; FONSECA, P. H. F.; CAIXETA, F. Aspectos fisiológicos e bioquímicos da germinação de sementes de alface em diferentes temperaturas. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 4, p. 316-322, abr. 2014. Título em inglês: Physiological and biochemical aspects of lettuce seed germination at different temperatures.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 14. | | CUNHA, A. T. M.; CARVALHO, J. O.; GUIMARÃES, A. L. S.; LEMEID, L. O.; CAIXETA, F. M.; VIANA, J. H. M.; DODE, M. A. N. Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time. PLoS ONE, v. 14, n. 1, article e0209692, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | ABREU, G. F. de; ROSA, S. D. V. F. da; CAIXETA, F.; CLEMENTE, A. da C. S.; PEREIRA, C. C.; COELHO, S. V. B. Colorimetria em grãos de café submetidos a diferentes métodos de processamento e armazenados em ambiente resfriado. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 16. | | DIAS, L. R. O.; SILVA, V. A. O.; FARIA, O. A. C.; CAIXETA, F. M. C.; SPRÍCIGO, J. F. W.; DODE, M. A. N. Embryos production from bovine oocytes matured in vivo using intrafollicular transfer of immature oocytes (TIFOI). Animal Reproduction, v. 15, n. 3, p. 428, Jul./Sept. 2018. Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | DODE, M. A. N.; CAIXETA, F. M. C.; VARGAS, L. N.; LEME, L. O.; KAWAMOTO, T. S.; FIDELIS, A. A. G.; FRANCO, M. M. Genome transfer technique for bovine embryo production using the metaphase plate and polar body. Journal of Assisted Reproduction and Genetics, v. 40, p. 943-951, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | CLEMENTE, A. C. S.; GUIMARÃES, R. M.; MARTINS, D. C.; GOMES, L. A. A.; CAIXETA, F.; REIS, R. G. E.; ROSA, S. D. V. F. da. Expression of genes associated with the biosynthetic pathways of abscisic acid, gibberellin, and ethylene during the germination of lettuce seeds. Genetics and Molecular Research, v. 14, n. 2, p. 4703-4715, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| 19. | | SILVA-NETA, L. C.; PINHO, E. V.; VEIGA, A. D.; PINHO, R. G.; GUIMARÃES, R. M.; CAIXETA, F.; SANTOS, H. O.; MARQUES, T. L. Expression of genes related to tolerance to low temperature for maize seed germination. Genetics and Molecular Research, v. 14, n, 1, p. 2674-2690, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 20. | | SILVA, V. A.; FARIA, O. A. C.; DIAS, L. R. O.; CAIXETA, F. M. C.; SPRÍCIGO, J. F. W.; DODE, M. A. N. Inhibition of meiosis resumption in bovine oocytes to be used in the intrafollicular transfer of immature oocyte (TIFOI). Animal Reproduction, v. 15, n. 3, p. 456, Jul./Sept. 2018. Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 34 | |
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