Data/hora: 06/06/2024 01:04:43

Biblioteca(s): 	Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais.	
Data corrente: 	14/03/2014	
Data da última atualização: 	26/01/2018	
Autoria: 	ALMEIDA, I. G.; IANELLA, P.; FARIA, M. T.; PAIVA, S. R.; CAETANO, A. R.	
Afiliação: 	PATRICIA IANELLA, DPD.	
Título: 	Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers.	
Ano de publicação: 	2013	
Fonte/Imprenta: 	Genetics and Molecular Research v. 12, n. 4, p. 6299-6308, Dec. 2013.	
DOI: 	http://dx.doi.org/10.4238/2013.December.4.17	
Idioma: 	Inglês	
Conteúdo: 	Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual dimorphism. Simple and inexpensive methods for sexing immature pirarucu based on DNA markers would facilitate production of this species in commercial operations. We analyzed A. gigas male and female DNA pools with 566 RAPD primers, generating 2609 fragments, with an estimated 1341 segregating polymorphic markers, and an estimated average spacing of 714 kb, which corresponds to less than 0.1% of the species' genome. Two putative sex-specific fragments were initially identified in bulked samples; but they were not confirmed in a study of individual male and female samples. We suggest that A. gigas has developed a non-chromosomal system of sex determination or, alternatively, that the species has undergone a recent loss of the chromosome carrying the sex-determining locus.	
Palavras-Chave: 	Marcadores moleculares; Peixe neotropical; RAPD.	
Thesagro: 	Genoma; Marcador molecular; Peixe de água doce; Pirarucu.	
Thesaurus Nal: 	Arapaima gigas; Freshwater fish; Genetic markers; Genome.	
Categoria do assunto: 	--
G Melhoramento Genético
L Ciência Animal e Produtos de Origem Animal	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/171706/1/gmr3157.pdf
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/99211/1/gmr3157.pdf	
Registro original: 	Embrapa Unidades Centrais (AI-SEDE)	
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