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Registro Completo |
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
18/02/2019 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LIMA, L. R.; MOMBACH, M. A.; PEDREIRA, B. C. e; FERREIRA, D. C.; CARVALHO, P. de; AMORIM, K. R. R. de; WEIMER, P. J.; CABRAL, L. da S. |
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Afiliação: |
LENI RODRIGUES LIMA, UFMT, CUIABA, MT; MIRCÉIA ANGELE MOMBACH, UFMT, CUIABA, MT; BRUNO CARNEIRO E PEDREIRA, CPAMT; DANIELA CRISTINA FERREIRA, UFMT, CUIABA, MT; PERIVALDO DE CARVALHO, UFMT, CUIABA, MT; KARITHA REGIANE RIBEIRO DE AMORIM, UFMT, CUIABA, MT; PAUL JAMES WEIMER, UNIVERSITY OF WISCONSIN, MADISON, US; LUCIANO DA SILVA CABRAL, UFMT, CUIABA, MT. |
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Título: |
Methanogens and rumen protozoa in cattle during transition to high grain diets. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais. Viçosa: Sociedade Brasileira de Zootecnia; Brasília, DF: Associação Brasileira de Zootecnistas. |
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Páginas: |
não paginado. |
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Idioma: |
Inglês |
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Conteúdo: |
The rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) were only reduced for animals fed with 90% concentrate diet (4.00) compared to other diets (forage, 50, 60, 70 and 80%, 5.07 log10 genes copies). The decrease on protozoa numbers may be related to rumen pH, in which protozoa numbers drop 21% only when rumen pH was lower that 5.8 (80% concentrate in the diets). However, the methanogens numbers were higher in forage fed animals (4.40 log10 genes copies) that all grains diets (3.71 log10 genes copies). Thus, there was not a clear association between protozoa and methanogens numbers in the rumen, what has also been observed in other studies done recently using molecular tools. Although we did not have any data about methane emissions in this study, we could speculate the higher concentrate diets (more than 50% concentrate), there would be a lower methane emission, considering the low methanogens numbers in the rumen. MenosThe rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) we... Mostrar Tudo |
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Thesaurus Nal: |
Archaea; Methanogens; Rumen microorganisms. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192971/1/2018-cpamt-bruno-pedreira-methanogens-rumen-protozoa-cattle-transition-grains-diets.pdf
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Marc: |
LEADER 03328nam a2200241 a 4500
001 2106183
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
100 1 $aLIMA, L. R.
245 $aMethanogens and rumen protozoa in cattle during transition to high grain diets.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais. Viçosa: Sociedade Brasileira de Zootecnia; Brasília, DF: Associação Brasileira de Zootecnistas.$c2018
300 $anão paginado.
520 $aThe rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) were only reduced for animals fed with 90% concentrate diet (4.00) compared to other diets (forage, 50, 60, 70 and 80%, 5.07 log10 genes copies). The decrease on protozoa numbers may be related to rumen pH, in which protozoa numbers drop 21% only when rumen pH was lower that 5.8 (80% concentrate in the diets). However, the methanogens numbers were higher in forage fed animals (4.40 log10 genes copies) that all grains diets (3.71 log10 genes copies). Thus, there was not a clear association between protozoa and methanogens numbers in the rumen, what has also been observed in other studies done recently using molecular tools. Although we did not have any data about methane emissions in this study, we could speculate the higher concentrate diets (more than 50% concentrate), there would be a lower methane emission, considering the low methanogens numbers in the rumen.
650 $aArchaea
650 $aMethanogens
650 $aRumen microorganisms
700 1 $aMOMBACH, M. A.
700 1 $aPEDREIRA, B. C. e
700 1 $aFERREIRA, D. C.
700 1 $aCARVALHO, P. de
700 1 $aAMORIM, K. R. R. de
700 1 $aWEIMER, P. J.
700 1 $aCABRAL, L. da S.
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Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
29/11/1993 |
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Data da última atualização: |
20/07/2020 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
BATISTA, H. A. M.; ALBUQUERQUE, M. de; CAMARAO, A. P.; LOURENÇO JUNIOR, J. de B. |
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Afiliação: |
HERIBERTO ANTONIO MARQUES BATISTA, CPATU; MILTON DE ALBUQUERQUE, CPATU; ARI PINHEIRO CAMARAO, CPATU; JOSE DE BRITO LOURENCO JUNIOR, CPATU. |
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Título: |
Suplementação alimentar com resíduos da agroindústria em bubalinos na fase de crescimento. |
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Ano de publicação: |
1983 |
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Fonte/Imprenta: |
Belém, PA: EMBRAPA-CPATU, 1983. |
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Páginas: |
2 p. |
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Série: |
(EMBRAPA-CPATU. Comunicado técnico, 44). |
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Idioma: |
Português |
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Conteúdo: |
Objetivando determinar o nível ideal para aproveitamento do farelo de rama de mandioca na suplementação alimentar de bubalinos em crescimento, foi conduzido no Laboratório de Nutrição Animal do CPATU-EMBRAPA um experimento, utilizando trinta e dois bezerros desmamados, com idade média de dez meses, os quais foram reunidos em oito grupos de quatro animais formados por duas fêmeas e dois machos, em delineamento jinteiramente casualizado. |
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Palavras-Chave: |
Agroindustria; Agroindustrial complexes; Bubalino; Bubalinos; Bufalos; Buffalo; Feed; Residue; Residuos; Restolhos; Restos; Suplementação alimentar; Supplements. |
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Thesagro: |
Alimentação; Alimentação Suplementar; Bubalus Bubalis; Búfalo; Crescimento; Ganho de Peso; Nutrição; Resíduo; Resíduo Industrial; Suplemento Alimentar. |
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Thesaurus NAL: |
nutrition; weight gain. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/59257/1/CPATU-ComTec44.pdf
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Marc: |
LEADER 01691nam a2200469 a 4500
001 1382985
005 2020-07-20
008 1983 bl uuuu u0uu1 u #d
100 1 $aBATISTA, H. A. M.
245 $aSuplementação alimentar com resíduos da agroindústria em bubalinos na fase de crescimento.
260 $aBelém, PA: EMBRAPA-CPATU$c1983
300 $a2 p.
490 $a(EMBRAPA-CPATU. Comunicado técnico, 44).
520 $aObjetivando determinar o nível ideal para aproveitamento do farelo de rama de mandioca na suplementação alimentar de bubalinos em crescimento, foi conduzido no Laboratório de Nutrição Animal do CPATU-EMBRAPA um experimento, utilizando trinta e dois bezerros desmamados, com idade média de dez meses, os quais foram reunidos em oito grupos de quatro animais formados por duas fêmeas e dois machos, em delineamento jinteiramente casualizado.
650 $anutrition
650 $aweight gain
650 $aAlimentação
650 $aAlimentação Suplementar
650 $aBubalus Bubalis
650 $aBúfalo
650 $aCrescimento
650 $aGanho de Peso
650 $aNutrição
650 $aResíduo
650 $aResíduo Industrial
650 $aSuplemento Alimentar
653 $aAgroindustria
653 $aAgroindustrial complexes
653 $aBubalino
653 $aBubalinos
653 $aBufalos
653 $aBuffalo
653 $aFeed
653 $aResidue
653 $aResiduos
653 $aRestolhos
653 $aRestos
653 $aSuplementação alimentar
653 $aSupplements
700 1 $aALBUQUERQUE, M. de
700 1 $aCAMARAO, A. P.
700 1 $aLOURENÇO JUNIOR, J. de B.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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