Data/hora: 16/06/2024 02:32:20

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 20/09/2001
Data da última atualização: | 20/09/2001
Autoria: | HERNANDEZ, L.; SOTOLONGO, M.; ROSABAL, Y.; MENENDEZ, C.; RAMIREZ, R.; CABALLERO-MELLADO, J.; ARRIETA, J.
Título: | Structural levansucrase gene (lsd A) constitutes a functional locus conserved in the species gluconacetobacter diazotrophicus.
Ano de publicação: | 2000
Fonte/Imprenta: | Archives of Microbiology, London, v. 174, p. 120-124, 2000.
Idioma: | Inglês
Conteúdo: | Levansucrase (EC 2.4.1.10) was identified as a  constitutive exoenzyme in 14 Gluconacetobacter diazotrophicus strains recovered from different host plants in diverse geographical regions. The enzyme, consisting of a single 60-kDa polypeptide, hydrolysed sucrose to synthesise oligofructans and levan. Sugar-cane-associated strains of the most abundant genotype (electrophoretic type 1) showed maximal values of levansucrase production. These values were three-fold higher than those of the isolates recovered from coffe plants. Restriction fragment length polymorphism analysis revealed a high degree of conservation of the levansucrase locus (lsdA) among the 14 strains under study, which representes 11 different G. diazotrophicus genotypes. Targeted disruption of the lsdA. gene in four representative strains abolished their ability to grow on sucrose, indicating that the endophytic species G.diazotrophicus utilises plant sucrose via levansucrase.
Palavras-Chave: | Acetobacter diazotrophicus; Cana-de-açúcar; Endofito.
Thesagro: | Bactéria; Eletroforese.
Thesaurus Nal: | electrophoresis; endophytes; Gluconacetobacter diazotrophicus; sugarcane.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (24661-1) - ADD - SP - -- -  -  - 22471 - DIS