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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/10/2020 |
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Data da última atualização: |
19/03/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEIXEIRA, N. C.; WENDLAND, A.; OLIVEIRA, M. I. de S.; BRANDÃO, L. T. D.; SOUZA, T. L. P. O. de; FARIA, J. C. de; DEL PELOSO, M. J.; CORTES, M. V. de C. B. |
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Afiliação: |
NARA CRISTINA TEIXEIRA, bolsista CNPAF; ADRIANE WENDLAND FERREIRA, CNPAF; MAYTHSULENE INACIO DE SOUZA OLIVEIRA, bolsista CNPAF; LIVIA TEIXEIRA DUARTE BRANDAO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF. |
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Título: |
Rapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Crop Science, v. 60, n. 6, p. 3004-3012, Nov./Dec. 2020. |
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ISSN: |
1435-0653 |
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DOI: |
https://doi.org/10.1002/csc2.20107 |
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Idioma: |
Inglês |
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Conteúdo: |
Amolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry. MenosAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaO... Mostrar Tudo |
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Thesagro: |
DNA; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Planta Transgênica. |
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Thesaurus Nal: |
Bean golden mosaic virus; Beans; Loop-mediated isothermal amplification; RNA interference; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02809naa a2200349 a 4500
001 2125441
005 2021-03-19
008 2020 bl uuuu u00u1 u #d
022 $a1435-0653
024 7 $ahttps://doi.org/10.1002/csc2.20107$2DOI
100 1 $aTEIXEIRA, N. C.
245 $aRapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification.$h[electronic resource]
260 $c2020
520 $aAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry.
650 $aBean golden mosaic virus
650 $aBeans
650 $aLoop-mediated isothermal amplification
650 $aRNA interference
650 $aTransgenic plants
650 $aDNA
650 $aFeijão
650 $aMosaico Dourado
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
700 1 $aWENDLAND, A.
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aBRANDÃO, L. T. D.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aFARIA, J. C. de
700 1 $aDEL PELOSO, M. J.
700 1 $aCORTES, M. V. de C. B.
773 $tCrop Science$gv. 60, n. 6, p. 3004-3012, Nov./Dec. 2020.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 87 | |
| 10. |  | SANTOS, T. D. dos; FERREIRA, E. P. de B.; KNUPP, A. M.; CORTES, M. V. de C. B. Caracterização de bactérias diazotróficas obtidas em diferentes regiões produtoras de feijoeiro-comum. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. FertBio 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 12. | | SPERANDIO, E. M.; FILIPPI, M. C. C. de; CORTES, M. V. de C. B.; VALE, H. M. M. do. Quantificação de B-1,3-glucanase e ácido salicílico em plantas de arroz induzidas com isolado avirulento de Magnaporthe oryzae. In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. p. 91. (Embrapa Arroz e Feijão. Documentos, 311).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 13. | | TEIXEIRA, N. C.; DUARTE, L. T.; CORTES, M. V. de C. B.; FILIPPI, M. C. C. de; WENDLAND, A. Levantamento dos principais patógenos de importância econômica encontrados em sementes de arroz. In: SEMINÁRIO JOVENS TALENTOS, 9., 2015, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. p. 117. (Embrapa Arroz e Feijão. Documentos, 309).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 14. | | CORREIA, F. R.; PEREIRA, R.; WENDLAND, A.; CORTÊS, M. V. de C. B.; SOUZA, T. L. P. O. de. Isolamento e identificação do fungo Colletotrichum lindemuthianum em amostras de feijoeiro-comum coletadas em Goiás e Sergipe durante 2013. In: SEMINÁRIO JOVENS TALENTOS, 8., 2014, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2014. p. 71. (Embrapa Arroz e Feijão. Documentos, 306).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 16. | | TEIXEIRA, N. C.; DUARTE, L. T.; CORTES, M. V. de C. B.; FILIPPI, M. C. C. de; WENDLAND, A. Desenvolvimento de iniciadores para detecção e diferenciação de Magnaporthe oryzae em sementes de arroz. In: SEMINÁRIO JOVENS TALENTOS, 9., 2015, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. p. 115. (Embrapa Arroz e Feijão. Documentos, 309).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 18. | | ARRIEL, M. T.; CORTES, M. V. de C. B.; SILVA-LOBO, V. L.; FILIPPI, M. C. C. de. Determinação da taxa de crescimento e relação C:N para o cultivo de Burkholderia pyrrocinia, bioagente com potencial para suprimir a brusone no arroz. In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. p. 88. (Embrapa Arroz e Feijão. Documentos, 311).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| Registros recuperados : 87 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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