Data/hora: 18/06/2024 14:46:32

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 13/10/2020
Data da última atualização: | 19/03/2021
Autoria: | TEIXEIRA, N. C.; WENDLAND, A.; OLIVEIRA, M. I. de S.; BRANDÃO, L. T. D.; SOUZA, T. L. P. O. de; FARIA, J. C. de; DEL PELOSO, M. J.; CORTES, M. V. de C. B.
Afiliação: | NARA CRISTINA TEIXEIRA, bolsista CNPAF; ADRIANE WENDLAND FERREIRA, CNPAF; MAYTHSULENE INACIO DE SOUZA OLIVEIRA, bolsista CNPAF; LIVIA TEIXEIRA DUARTE BRANDAO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF.
Título: | Rapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification.
Ano de publicação: | 2020
Fonte/Imprenta: | Crop Science, v. 60, n. 6, p. 3004-3012, Nov./Dec. 2020.
ISSN: | 1435-0653
DOI: | https://doi.org/10.1002/csc2.20107
Idioma: | Inglês
Conteúdo: | Amolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry.
Thesagro: | DNA; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Planta Transgênica.
Thesaurus Nal: | Bean golden mosaic virus; Beans; Loop-mediated isothermal amplification; RNA interference; Transgenic plants.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (35923-1) - UPC - AP - DD - 2020 -  - 2020 - DIS