Data/hora: 19/06/2024 19:05:38

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 07/03/2019
Data da última atualização: | 07/11/2019
Autoria: | ANDRADE, I. R. A. de; CÂNDIDO, M. J. D.; POMPEU, R. C. F. F.; FEITOSA, T. S.; BOMFIM, M. A. D.; SALLES, H. O.; EGITO, A. S. do.
Afiliação: | IGO RENAN ALBUQUERQUE DE ANDRADE, Instituto Federal de Educação, Ciência e Tecnologia do Ceará (IFCE) - Campus Crateús - Crateús, CE, Brazil; MAGNO JOSÉ DUARTE CÂNDIDO, Universidade Federal do Ceará (UFC) - Fortaleza, CE, Brazil; ROBERTO CLAUDIO FERNANDES F POMPEU, CNPC; TIBÉRIO SOUSA FEITOSA, ESALQ / USP- Piracicaba, SP, Brazil; MARCO AURELIO DELMONDES BOMFIM, CNPC; HEVILA OLIVEIRA SALLES, CNPC; ANTONIO SILVIO DO EGITO, CNPC.
Título: | Inactivation of lectins from castor cake by alternative chemical compounds.
Ano de publicação: | 2019
Fonte/Imprenta: | Toxicon, v. 160, p. 47-54, Feb. 2019.
DOI: | 10.1016/j.toxicon.2019.02.003
Idioma: | Inglês
Conteúdo: | Abstract: Enabling the use of castor cake in animal feeding is an excellent alternative strategy to reduce feed costs. The cake is a by-product derived from the extraction of the castor oil by the biodiesel industry whose chemical composition is satisfactory despite the presence of antinutritional factors like toxic lectins, which require detoxification before it can be used as a dietary ingredient. The aim of the present study was to evaluate alternative chemical sources in the degradation and inactivation of ricin and Ricinus communis agglutinin (RCA), two lectins from castor cake. Ten chemical compounds were evaluated: sodium hydroxide, monodicalcium phosphate, dicalcium phosphate, calcium oxide, calcium hydroxide, calcitic limestone, magnesian limestone, urea, potassium chloride, and sodium chloride. Gel electrophoresis indicated 100% lectin degradation only in the cakes treated with 90 g sodium hydroxide and 2500 mL water per kg of cake. The hemagglutination assay was crucial to providing innocuousness to the treated cakes, with total absence of hemagglutinating activity observed in the castor cakes treated with 60 or 90 g sodium hydroxide in water volumes equal to or higher than 1500 mL/kg of castor cake and in the cakes treated with 90 g calcium oxide with 2500 or 3000 mL water/kg castor cake. Thus, though depending on the concentration of the chemical compound and on the volume of water per kilogram of treated cake, sodium hydroxide and calcium oxide showed to be promising chemical products for degradation and complete inactivation of the lectins present in castor cake to allow its use as an ingredient in animal diets.
Palavras-Chave: | Haemagglutination tests; Hemagglutinating activity; Ricinus agglutinin.
Thesagro: | Ricinus Communis.
Thesaurus Nal: | Animal feeding; Calcium oxide; Denaturation; Detoxification (processing); Electrophoresis; Lectins; Ricin; Sodium hydroxide.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (38916-1) - UPC - AP - DD -  -  -  - DIS