Data/hora: 21/06/2024 05:21:15

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 15/04/2004
Data da última atualização: | 15/04/2004
Autoria: | SCHÖPKE, C.; TAYLOR, N. J.; MASONA, M. V.; CÁRCAMO, R.; HO, T.; BEACHY, R. N.; FAUQUET, C. M.
Título: | Characterization of transgenic cassava plants containing the coat protein gene of cassava common mosaic virus.
Ano de publicação: | 1998
Fonte/Imprenta: | Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 33, nov., 1998. Suplemento.
Idioma: | Inglês
Conteúdo: | Abstract: During recent years several groups were successful in genetically transforming cassava. While initially marker genes were used to optimize transformation protocols, work is now focusing on the transfer of genes of interest into this crop. One of our goals is to apply the coat protein-mediated resistance strategy to produce cassava plants resistant to a virus originating from South America, cassava common mosaic virus (CsCMV). Gene constructs containing the neophosphomycin transferase gene (nptll) as selectable marker and the coat protein gene of CsCMV were transferred through particle bombardment into embryogenic suspension - derived tissue of cassava cultivar TMS 60444. Bombarded tissues were selected with paromomycin, and after regeneration of selected tissue through somatic embryogenesis eventually more than thirty transgenic plant lines were established. CsCMV coat protein was detected through Western  blot analysis in nine of these lines. Data will be presented on the production of the transgenic plants and on their general and molecular characterization.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (20969-1) - ADD - AP - -- - 633.68205 -  -  - DIS