Data/hora: 25/06/2024 13:10:04

Biblioteca(s): | Embrapa Instrumentação; Embrapa Semiárido.
Data corrente: | 28/09/2018
Data da última atualização: | 03/10/2018
Autoria: | FERREIRA, L. M. B.; ALONSO, J. D.; KIILL, C. P.; FERREIRA, N. N.; BUZZÁ, H. H.; GODOI, D. R. M. de; BRITTO, D. de; ASSIS, O. B. G. de; SERAPHIM, T. V.; BORGES, J. C.; GREMIÃO, M. P. D.
Afiliação: | DOUGLAS DE BRITTO, CPATSA; ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA.
Título: | Exploiting supramolecular interactions to produce bevacizumab-loaded nanoparticles for potential mucosal delivery.
Ano de publicação: | 2018
Fonte/Imprenta: | European Polymer Journal, v. 103, p. 238-250, jun. 2018.
Páginas: | 238-250
DOI: | 10.1016/j.eurpolymj.2018.04.013
Idioma: | Inglês
Conteúdo: | Monoclonal antibody (mAb) delivery is gaining importance for local, systemic, and route-speci&#64257;c targeting. Themucus constitutes the main barrier for this type of delivery. In the present study, we aimed to develop a drugdelivery platform by integrating mucus penetrating and mucoadhesive agents into a single system. Our hy-pothesis is that by combining these opposing functions, this system could have its properties modulated ac-cording to speci&#64257;c purposes. Self-assembly studies were conducted ung three classes of building blocks: the protein drug bevacizumab (BVZ), mucus-penetrating polyanion dextran sulfate (DS), mucoadhesive polycations trimethylchitosan (TMC) and chitosan oligosaccharides (COS). We obtained two types of nanoarticles by manipulating supramolecular interactions between the components. Binary protein-polyanion (BVZ/DS) nano-particles showed size of approximately 150 nm and a negative zeta potential. Ternary protein-polyanion-poly-cation (BVZ/DS/COS) nanoparticles were obtained using COS and exhibited 350 nm and a positive zeta potential. Assisted by calorimetric information, we demonstrated that building stable ternary nanoparticles carrying positive charges were not possible using the polycation TMC due to its thermodynamic onstraints. Furthermore, spectroscopy analysis and CAM assay indicated that BVZ continued structurally and functionally stable after its incorporation into the nanoparticles. These two types of nanoparticles exhibited di&#64256;erent be-haviors when interacting with mucin, as shown by DLS and AFM studies. While the negatively charged particles promoted dispersion of the mucin network, suggesting a mucus penetrating e&#64256;ect of DS, the positively chargedparticles formed aggregates, probably caused by the mucoadhesive e&#64256;ect of COS. These results highlight the importance of understanding the role of supramolecular interactions, responsible for forming drug delivery systems containing complex molecules, such as proteins and polymers.
Palavras-Chave: | Bevacizumab; Drug delivery platform; Interações supramoleculares; Mucoadesão; Mucoadhesion; Nanoparticula; Nanopartículas poliméricas; Penetração do muco; Plataforma de entrega de medicamentos; Polymeric nanoparticles; Supramolecular interactions.

Categoria do assunto:

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X Pesquisa, Tecnologia e Engenharia

Registro original: | Embrapa Semiárido (CPATSA)

Exemplares:

CNPDIA - (16816-1) - UPC - AP - DD - PROCI.18.0052 -  - 2018.0078 - DIS
CPATSA - (57532-1) - UPC - AP - DD -  -  -  - DIS