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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
01/06/1994 |
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Data da última atualização: |
01/06/1994 |
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Autoria: |
BURGER, D. W. |
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Título: |
Guidelines for autoclaving liquid media used in plant tissue culture. |
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Ano de publicação: |
1988 |
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Fonte/Imprenta: |
Horscience, v.23, n.6, p.1066-1068, 1988. |
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Idioma: |
Inglês |
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Conteúdo: |
The temperatures of liquid volumes (10-4000 ml) were monitored with solid-state temperature probes during autoclaving cycles of an automated steam sterilizer. Accurate measurements of the time required for the liquid volumes to reach the sterilizing temperature (121oC) were made. The effects of preheating solutions to be autoclaved and enclosing materials in autoclavable plastic bags were determined. Liquid volumes between 10 and 4000 ml differed in the time required to reach 121oC by 40 min. Preheating solutions before autoclaving slightly reduced the time required to reach sterilizing temperatures. Enclosing materials in autoclavable bags substantially increased the time required to reach 121o. |
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Palavras-Chave: |
Autoclavagem; Cultura de tecidos. |
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Thesagro: |
Esterilização; Meio de Cultura. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01167naa a2200169 a 4500
001 1418069
005 1994-06-01
008 1988 bl --- 0-- u #d
100 1 $aBURGER, D. W.
245 $aGuidelines for autoclaving liquid media used in plant tissue culture.
260 $c1988
520 $aThe temperatures of liquid volumes (10-4000 ml) were monitored with solid-state temperature probes during autoclaving cycles of an automated steam sterilizer. Accurate measurements of the time required for the liquid volumes to reach the sterilizing temperature (121oC) were made. The effects of preheating solutions to be autoclaved and enclosing materials in autoclavable plastic bags were determined. Liquid volumes between 10 and 4000 ml differed in the time required to reach 121oC by 40 min. Preheating solutions before autoclaving slightly reduced the time required to reach sterilizing temperatures. Enclosing materials in autoclavable bags substantially increased the time required to reach 121o.
650 $aEsterilização
650 $aMeio de Cultura
653 $aAutoclavagem
653 $aCultura de tecidos
773 $tHorscience$gv.23, n.6, p.1066-1068, 1988.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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| 4. |  | CHAMONE, M. E.; STEPHAN, M. P.; AZEVEDO, T. de L.; ASCHERI, J. L. R.; ROSA, J. S. da; CASTRO, I. M. de. Detecção de alcaloides em tremoço branco (Lupinus albus ) na forma de farinha, concentrados proteicos e ligados a proteínas purificadas por SDS-PAGE. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 28., 2022. Anais... [Online]: SBCTA; Embrapa, 2022. Evento online. 5 p. 22 a 25 de novembro de 2022.| Tipo: Artigo em Anais de Congresso |
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