Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
08/10/2009 |
Data da última atualização: |
04/07/2016 |
Autoria: |
LIMA-VERDE, I. B.; MATOS, M. H. T.; BRUNO, J. B.; MARTINS, F. S.; SANTOS, R. R.; BÁO, S. N.; LUQUE, M. C. A.; VIEIRA, G. A. B.; SILVEIRA, E. R.; RODRIGUES, A. P. R.; FIGUEIREDO, J. R.; OLIVEIRA, M. A. L.; LIMA, P. F. |
Título: |
Effects of alpha-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 61, n. 1, p. 57-65, abr. 2009. |
Idioma: |
Inglês |
Conteúdo: |
The effects of á-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without á-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15ìM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that á-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture. |
Palavras-Chave: |
Folículo. |
Thesagro: |
Antioxidante; Caprino; Hormônio; Reprodução Animal. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02210naa a2200325 a 4500 001 1533641 005 2016-07-04 008 2009 bl uuuu u00u1 u #d 100 1 $aLIMA-VERDE, I. B. 245 $aEffects of alpha-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured. 260 $c2009 520 $aThe effects of á-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without á-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15ìM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that á-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture. 650 $aAntioxidante 650 $aCaprino 650 $aHormônio 650 $aReprodução Animal 653 $aFolículo 700 1 $aMATOS, M. H. T. 700 1 $aBRUNO, J. B. 700 1 $aMARTINS, F. S. 700 1 $aSANTOS, R. R. 700 1 $aBÁO, S. N. 700 1 $aLUQUE, M. C. A. 700 1 $aVIEIRA, G. A. B. 700 1 $aSILVEIRA, E. R. 700 1 $aRODRIGUES, A. P. R. 700 1 $aFIGUEIREDO, J. R. 700 1 $aOLIVEIRA, M. A. L. 700 1 $aLIMA, P. F. 773 $tArquivo Brasileiro de Medicina Veterinária e Zootecnia$gv. 61, n. 1, p. 57-65, abr. 2009.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|